A High Resolution CCD Intravital Fluorescent Microscope for Observation of Coronary Smaller Microvessels in a Beating Heart

用于观察跳动心脏中冠状动脉较小微血管的高分辨率 CCD 活体荧光显微镜

• 批准号: 05557043
• 负责人: KAJIYA Fumihiko
• 金额: $ 10.88万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05557043/
• 关键词: High Resolution CCD microscope; GRIN Lens; Coronary Circulation; Intramyocardial microvessel; Glomerulus; Intra-renal Microvessel; Needle-probe; Renal Microcirculation; ニードルプローブ; 高品位CCD撮像素子; 高品位テレビ; 輸入輸出動脈; 生理活性物質

We developed a needle-probe intravital microscope with CCD (charge coupled device), by which we can observe coronary microvessels (D>50 mum) in a beating heart with and without fluorescent dye (Circ.Res., 72 : 939-946,1993, Circ.Res.75 : 393-399,1994). To analyze smaller pre- and post-capillary microvessels, we developed a new high resolution CCD system, which consists of a needle probe, a camera body, a lens, a light guide and source, a high definition monitor and a high definition videocassette recorder. The camera has a high resolution CCD image sensor with 1940 x 1040 pixels. The needle-probe contains a relay lens (diameter : 4 mm ; length : 140 mm) to increase resolution. Images focused on the CCD through a relay lens are converted to high definition video signals every 33 ms. To examine the accuracy of this system, a micro scale pattern was observed. The spatial resolution was about 2.5 mum for the x200objective. We could clearly observe small pre- and post-capillary microvessels in subepicardium in a beating heart, which was difficult by our preview system. We also apllied our system to visualize renal microcirculation and found that the images of afferent and efferent arterioles were clealy observed by our system. In conclusion, our new high resolution needle-probe CCD intravital microscope is a powerful tool to observe small pre- and post-capillary microvessels (D<50 mum) in a beating heart.

我们开发了一种具有CCD（电荷耦合器件）的针探针活体显微镜，通过该显微镜，我们可以观察具有和不具有荧光染料的跳动心脏中的冠状动脉微血管（D>50 μ m）（Circ.Res.，72：939- 946，1993，Circ.Res.75：393- 399，1994）。为了分析更小的前和后毛细血管，我们开发了一种新的高分辨率CCD系统，该系统由针探头，相机体，透镜，光导和光源，高清晰度监视器和高清晰度录像机组成。该相机具有1940 x 1040像素的高分辨率CCD图像传感器。探针包含中继透镜（直径：4 mm ;长度：140 mm）以增加分辨率。通过中继透镜聚焦在CCD上的图像每隔33 ms被转换为高清晰度视频信号。为了检查该系统的精度，观察了微尺度图案。的空间分辨率为约2.5妈妈的x200物镜。我们可以清楚地观察到小的前和后毛细血管在心外膜在跳动的心脏，这是困难的预览系统。我们还将该系统应用于肾微循环的显示，发现该系统能清晰地观察到肾的输入和输出小动脉的图像。总之，我们的新的高分辨率针探头CCD活体显微镜是一个强大的工具，观察小前和后毛细血管微血管（D<50妈妈）在跳动的心脏。

项目成果

Tokunori Yamamoto,et al.: "Observation of in Vivo human dye excretion in a single nephron vsing a nsedle-probe CCD videomicroscope" Circulation.

Tokunori Yamamoto 等人：“使用单探针 CCD 视频显微镜观察单个肾单位体内人类染料排泄”循环。

矢田豊隆、他: "反応性充血反応時の拍動下心内膜側冠細動脈に及ぼす影響について" 脈管学. 33. 760 (1993)

Toyotaka Yada 等人：“反应性充血反应期间对心内膜冠状动脉搏动的影响”Angiology 33. 760 (1993)。

Toyotaka Yada,et al.: "Effect of nitroglycerin on diameter and pulsation amplitude of subendocardial arterides in beating porcine heart" American Journal of Physiology. 267. H1719-H1725 (1994)

Toyotaka Yada 等人：“硝酸甘油对猪心脏跳动中心内膜下动脉直径和搏动幅度的影响”美国生理学杂志。

Yasuo Ogasawara et al: "A high resolusion CCD intravital microscope for observation of coronary smaller imcrovessels (D<50μm)in a beating heart" Circulation. 90. I265 (1994)

Yasuo Ogasawara 等人：“用于观察跳动心脏中冠状动脉较小微血管 (D<50μm) 的高分辨率 CCD 活体显微镜” 90. I265 (1994)。

Toyotaka Yada,et al.: "In vivo observation of subendocardial microvessels of the beating porcine heart using a needle-probe videomicroscope with a CCD camera" Circulation Research. 72. 939-946 (1993)

Toyotaka Yada 等人：“使用带有 CCD 摄像头的针头视频显微镜对跳动的猪心脏的心内膜下微血管进行体内观察”循环研究。