Interaction of coronary hemodynamical and biomolecular signals

冠状动脉血流动力学和生物分子信号的相互作用

• 批准号: 08044329
• 负责人: KAJIYA Fumihiko
• 金额: $ 9.86万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044329/
• 关键词: coronary circulation; intramyocardial microvessels; mechanical stress; tissue energetics; high-resolution icrocirculatory measurement; needle-probe CCD intravital microscopy; molecula biomechanics

We organized this interdisciplinary project in order to provide a better undestanding of the regulation of the hierarchically structured coronary systems to hemodynamical and metabolic stimuli. Followings are the major findings, implicatins, and suggestions for the future direction of the collaborative research on coronary circulation.1. With aids of needle-probe CCD intravital microscopy and coronary perfusion system, we evaluated for the first time the microvascular behavior in the subendocardial myocardial layrs which is at greatest risk of ischemia in the heart by controlling coronary arterial hemodynamics.2. Not only subepicardial but also subendocardial arterioles showed rapid change in diameter in response to a change in coronary perfusion pressure.3. Immediate response of coronary endothelium-derived nitric oxide to a change in shear stress was evaluated in vivo and contribution of nitric oxide to the mutual regulation of pulsatile coronary hemodynamics and myocardial energeticfs was suggested.4. New mechanoenergetic evaluation of the myocardial performance was proposed in relation to the cellular energetics and molecular signaling. To incorporate myocardial energetics with coronary regulation in vivo, it was suggested taht moment of inertia during cardiac ejection should be taken into account.5. Since the responses of intramyocardial microcirculatory flow and pressure to the coronary hemodynamics are quicker than ever predicted, further improvement of the spatiotemporal resolutions of the measuring systems of the coronary microcirculation is necessary.

我们组织了这个跨学科的项目，以便更好地了解冠状动脉系统对血流动力学和代谢刺激的分级结构的调节。以下是主要发现、启示和对未来冠脉循环合作研究方向的建议。结果：1.借助针尖探针式CCD活体显微镜和冠脉血流灌注系统，我们首次通过控制冠状动脉血流动力学来评估心内膜下心肌层的微血管行为，这是心脏缺血风险最大的部分。不仅心外膜下小动脉和心内膜下小动脉的直径随着冠脉灌注压的改变而迅速改变。在体内评价冠脉内皮源性一氧化氮对切应力变化的即刻反应，并推测一氧化氮在脉动性冠脉血流动力学和心肌能量相互调节中的作用。从细胞能量学和分子信号转导的角度，提出了新的评价心肌功能的机械能学方法。为了将心肌能量学与体内冠状动脉调节结合起来，建议考虑心脏射血时的惯性矩。由于心肌内微循环流量和压力对冠状动脉血流动力学的响应比以往预测的更快，因此有必要进一步提高冠脉微循环测量系统的时空分辨率。

项目成果

Y.Ogasawara,F.Kajiya et al: "A high Speed CCD intravital video microscope -observation and analysis of microcirculation in a beating heart-" Biomedicil Engineering-Applications,Basis and Communications. 8. 332-335 (1996)

Y.Ogasawara、F.Kajiya 等人：“高速 CCD 活体视频显微镜 - 跳动心脏中微循环的观察和分析 -”生物医学工程 - 应用、基础和通信。

F.Kajiya: "Temporal and Spatial heterogeheity of blood supply to heart Visualization and interpretation" Methods of Information in Medicine. 36. 319-321 (1997)

F.Kajiya：“心脏血液供应的时间和空间异质性可视化和解释”医学信息方法。

Kajiya F: "Temporal and spatial heterogeneity of blood supply to the heart : visualization and interpretation" Methods of Information in Medicine. 36. 319-321 (1997)

Kajiya F：“心脏血液供应的时间和空间异质性：可视化和解释”医学信息方法。

K.Morita, F.Kajiya EO Feigl et al: "α-Adrenergic vasoconstriction reduces systolic retrograde cororary blood flow" American Journal of Physiology. 273. H2746-H2755 (1997)

K.Morita、F.Kajiya EO Feigl 等人：“α-肾上腺素血管收缩减少收缩期逆行冠状动脉血流”美国生理学杂志 273。H2746-H2755 (1997)

R.VanBibber,EO.Feigl et al: "Role of adenosine in norepinephrine-induced coronary vasodilation" American Journal of Physiology. 273. H557-H565 (1997)

R.VanBibber、EO.Feigl 等人：“腺苷在去甲肾上腺素诱导的冠状血管舒张中的作用”美国生理学杂志。