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Analysis on myocardial ischemia by observation of NADH fluorescence/oxygen partial pressure phosphorescence using an intra-vital needle microscope

活体针显微镜观察NADH荧光/氧分压磷光分析心肌缺血

基本信息

批准号：
10558140
负责人：
KAJIYA Fumihiko
金额：
$ 7.94万
依托单位：
Kawasaki Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

We made in-vivo analysis on images of metabolic function and oxygen partial pressures in endocardial microcirculation where is the most frequent site of myocardial ischaemia. The purpose of the present study was to elucidate coronary microcirculation pathophysiologically. We achieved ;1) development of needle lens2) development of an NADH excitor and its fluorescence observer(excitation at 370 nm, fluorescence at 460 mm)3) development of Pd-porphine excitor and its fluorescence observer(excitation at 545 nm, fluorescence at 610 nm)4) development of micro-circulation image observer(wave length : 545 nm)With these developments, we confirmed that myocardial metabolism imaging was made possible.After evaluating the performance of the developed intra-vital needle microscope, we firstly applied this system to porcine and canine experiments. Obtaining metabolism images by NADH fluorescence, capillary blood flow distribution of digital radiogram was measured by perfusing molecular blood flow tracers, which specifically bind to α2 receptors of capillary endothelial cells.Secondly, we performed metabolism imaging at the region of endocardial microcirculation under the conditions of control and myocardial ischaemia. We compared NADH fluorescence intensity, coronary micro-vascular diameters and blood flow distribution under the control condition of 100 mmHg perfusion pressure and under the ischaemic condition of low perfusion pressure at 40 and 60 mmHg. There was heterogeneity with patchy pattern in NADH fluorescence distribution during reperfusion and hypoxic perfusion.. The sizes of patchy area in both images are about several hundred microns. In summary, there is a significant correlation between NADH fluorescence and blood flow distribution, indicating the similarity between metabolic function distribution and blood flow distribution.

我们对心肌缺血最常见部位--血管内微循环的代谢功能和氧分压图像进行了在体分析。本研究的目的是阐明冠状动脉微循环的病理生理。我们做到了; 1）针透镜的开发2）NADH激发子及其荧光观察器的开发（激发波长370 nm，荧光波长460 mm）3）钯-卟吩激发子及其荧光观察者的开发（545 nm激发，610 nm荧光）4）微循环图像观察器的开发（波长：545 nm），我们证实了心肌代谢成像的可能性。在评估了所开发的活体针式显微镜的性能后，我们首先将该系统应用于猪和犬的实验。用NADH荧光法获得代谢显像，用与毛细血管内皮细胞α2受体特异性结合的分子血流示踪剂灌注法测定数字化X线片的毛细血管血流分布。我们比较了100 mmHg灌注压对照条件下和40和60 mmHg低灌注压缺血条件下的NADH荧光强度、冠状动脉微血管直径和血流分布。再灌注和低氧灌注时，NADH荧光分布呈斑片状不均一性。两幅图像中的斑片状区域的大小约为数百微米。综上所述，NADH荧光与血流分布存在显著相关性，表明代谢功能分布与血流分布具有相似性。