权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of contimous cell separation system using polymeric adsorbent with surface micro-structures

使用具有表面微结构的聚合物吸附剂开发连续细胞分离系统

基本信息

批准号：
05558118
负责人：
KATAOKA Kazunori
金额：
$ 3.33万
依托单位：
Science University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

The interaction of rat lymphocyte subpopulations with the surfaces of poly (2-hydroxyethyl methacrylate) -graft-polyamine copolymer (HA) and poly (2-hydroxyethyl methacrylate) -graft-partially quaternized polyamine copolymer (HQA) was evaluated under a controlled shear stress by using a plane-parallel type channel equipped with a polymer-coated bottom wall. The cells introduced into the channel were settled for a prescribed time period, followed by the exposure to a shear stress resulting from the parabolic flow in the channel. When the shear stress exceeded the strength of cell/surface interaction, the cells were able to be detached from the polymer surface. By this way, quantitative evaluation of cell/surface interaction was done in time-dependent manner to clarify the effect of quaternization of polyamine portion in graft copolymer on lymphocyte attachment. Extended settling time (Tsf) caused a progressive increase in the attachment force between lymphocyte and copolymer surface for HA channel, resulting in a considerable decrease in the recovery of both B and T cells from the channel. On the contrary, recovery of T cells from HQA channel showed only a slight decrease with a settling time, allowing to obtain T cell population as effluent in a good yield even under the condition with prolonged settling time. Time-dependent increase in cell/surface interaction observed for HA channel might be due to a progressive contribution of hydrophobic interaction caused by an increase in hydrophobicity of HA copolymer through the deprotonation of ethylenediamine units in polyamine grafts at cellular interface. These results indicated that lymphocyte attachment is able to be controlled by changing the amount and the nature (fixed our unfixed) of charge in polyamine graft copolymers.

采用包覆聚合物底壁的平面平行通道，研究了大鼠淋巴细胞亚群与聚甲基丙烯酸羟乙酯-接枝-多胺共聚物（HA）和聚甲基丙烯酸羟乙酯-接枝-部分季铵化多胺共聚物（HQA）表面在可控剪切应力下的相互作用。引入通道的细胞在规定的时间内固定，然后暴露在通道中抛物线流产生的剪切应力下。当剪切应力超过细胞/表面相互作用的强度时，细胞能够从聚合物表面分离。通过这种方式，以时间依赖的方式对细胞/表面相互作用进行定量评价，以阐明接枝共聚物中多胺部分季铵化对淋巴细胞附着的影响。延长的沉淀时间（Tsf）导致淋巴细胞与HA通道共聚物表面之间的附着力逐渐增加，导致B细胞和T细胞从通道中恢复的速度明显下降。相反，随着沉淀时间的延长，HQA通道中T细胞的回收率仅略有下降，即使在沉淀时间延长的情况下，也可以获得产量较高的出水T细胞群。观察到的HA通道细胞/表面相互作用的时间依赖性增加可能是由于细胞界面多胺接枝中乙二胺单元的去质子化导致HA共聚物的疏水性增加，从而导致疏水相互作用的逐步贡献。这些结果表明，淋巴细胞的附着可以通过改变多胺接枝共聚物中电荷的数量和性质（固定或不固定）来控制。