Creation of intelligent polymeric micelle nanocapsuls for gene delivery

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We investigated intelligent polymeric micelle nanocapsuls formed through the complexation of block copolymers and plasmid DNA, as non-viral gene vectors. The block copolymers are composed of a hydrophilic polyethylene glycol (PEG) and positively charged polyamino acids. To enhance the efficacy of gene expression, in particular in vivo system, novel strategy for the active targeting which can be realized through the introduction of pilot moiety on the surface of polymeric micelles should be established. For this purpose, we developed a synthetic scheme for block copolymers to employ multi-intelligent functions, i.e., prolonged circulation inside the blood vessel tubes, enhanced permeation from the blood vessel tubes toward tissues, selective transfection to the targeting tissues, enhanced cellular uptake due to the pilot moieties, smooth release of plasmid DNA inside the cells, etc.In addition, a wide adaptability for the length of plasmid DNA and also the environmental responsibility of the polymeric micelle nanocapsuls that should be essential to overcome the efficacy of viral gene delivery system were experimentally conformed. This system exhibits highly transfection potency not only in in vitro system but also in preliminary in vivo experiments. This multi-intelligent polymeric micelle nanocapsuls can be treated as an universal platform of non-viral gene delivery system.