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Biomedical approach of new materials with well-organized interfaces

具有组织良好的界面的新材料的生物医学方法

基本信息

批准号：
11694129
负责人：
KATAOKA Kazunori
金额：
$ 3.14万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

1) Nanostructure fabrication from block copolymers was carried out in this study. Particularly, novel approaches for the construction of functionalized poly(ethylene glycol) (PEG) layers on surfaces were focused to attain the specific adsorption of a target protein through a PEG-conjugated ligands with a minimal non-specific adsorption of other proteins. Further, surface organization of block copolymer micelles with cross-linking core was studied from a standpoint of the preparation of a new functional surface-coating with a unique macromolecular architecture. The micelle-attached surface and the thin hydrogel layer made by layered micelles exhibited nonfouling properties and worked as the reservoir for hydrophobic reagents. These PEG-functionalized surface in brush form or in micelle form can be used in diverse fields of medicine and biology to construct high-performance medical devices including scaffolds for tissue engineering and matrices for drug delivery systems.2) Recently, coll … More oidal carrier systems have been receiving much attention in the field of drug targeting because of their high loading capacity for drugs as well as of their unique disposition characteristics in the body. This research project was aimed at the utility of polymeric micelles formed through the multimolecular assembly of block copolymers as novel core-shell typed colloidal carriers for drug and gene targeting. The process of micellization in aqueous milieu is studied in detail based on the differences in the driving force of core segregation, including hydrophobic interaction, electrostatic interaction, metal complexation, and hydrogen bonding of constituent block copolymers. The segregated core embedded in the hydrophilic palisade is shown to function as a reservoir for genes, enzymes, and a variety of drugs with diverse characteristics. Functionalization of the outer surface of the polymeric micelle to modify its physicochemical and biological properties is investigated from the standpoint of designing micellar carrier systems for receptor-mediated drug delivery. Further, the body distribution of polymeric micelles is studied to demonstrate their long-circulating characteristics and significant tumor accumulation, emphasizing their promising utility in tumortargeting therapy. As an important perspective on carrier systems based on polymeric micelles, their feasibility as nonviral gene vectors is also demonstrated in this study. Less

1)在这项研究中，从嵌段共聚物的纳米结构的制造进行。特别地，用于在表面上构建官能化聚（乙二醇）（PEG）层的新方法集中于通过PEG缀合的配体实现对靶蛋白的特异性吸附，而对其他蛋白的非特异性吸附最小。此外，从制备具有独特大分子结构的新型功能性表面涂层的角度，研究了具有交联核的嵌段共聚物胶束的表面组织。胶束附着表面和由层状胶束制成的薄水凝胶层表现出无污染特性，并作为疏水试剂的储库。这些刷状或胶束状的PEG功能化表面可用于医学和生物学的各个领域，以构建高性能的医疗器械，包括用于组织工程的支架和用于药物递送系统的基质。2）最近，科尔 ...更多信息类载体系统由于其对药物的高负载能力以及其在体内独特的处置特性而在药物靶向领域中受到了很多关注。本研究旨在利用嵌段共聚物的多分子组装形成的聚合物胶束作为新型核壳型胶体载体，用于药物和基因靶向。基于嵌段共聚物的疏水相互作用、静电相互作用、金属络合作用和氢键作用等不同的核心分离驱动力，详细研究了嵌段共聚物在水溶液中的胶束化过程。嵌入亲水性栅栏中的分离核心被证明是基因、酶和具有不同特征的各种药物的储存库。功能化的聚合物胶束的外表面，以修改其物理化学和生物学性质的研究从设计胶束载体系统的角度为受体介导的药物递送。此外，研究了聚合物胶束的体内分布，以证明其长循环特性和显着的肿瘤蓄积，强调其在肿瘤靶向治疗中有前途的实用性。作为一个重要的角度对基于聚合物胶束载体系统，其作为非病毒基因载体的可行性也证明在这项研究中。少