STUDY ON LIVER CIRRHOSIS AND HEPATOCELLULAR CARCINOMA IN CHINA

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To estimate the seroepidemiology of HBV and HCV infection in China where schistosomiasis japonica was endemic, we performed the collaborative studies with West China University of Medical Sciences and Sichuan Institute of Parasitic Disease.A total of 1,268 serum samples from six general populations in China were tested for HBV and HCV markers. And we have studied 104 serum samples from the patients with liver cirrhosis and 138 samples with hepatocellular carcinoma in Sichuan, China.Among 519 serum samples from the endemic areas of schistosomiasis japonica, 56 (10.8%) were positive for HBsAg, and 164 (31.6%) were negative for all HBV markers. But Anti-HCV was not detected with a second-generation ELISA kit. Among 530 samples from three old endemic area, 79 (14.9%) were positive for HBsAg, and 135 (25.5%) were negative for all HBV markers. And 3 (0.57%) were positive for Anti-HCV.Among 219 samples from two nonendemic area, 18 (8.2%) were positive for HBsAg, 70 (32.0%) were negative for all HBV markers, and 36 (14.9%) were positive for Anti-HCV.In chronic liver diseases, HBsAg was detected in 170 cases (70.2%) from 242 examined cases. Anti-HCV was positive in 36 cases (14.9%).ELISA using soluble Schistosoma japonicum eggs antigen (SEA) was used for the screening of schistosomiasis. 339 (65.3%) out of 519 samples from the endemic area, 87 (16.4%) out of 530 from the old endemic area, 4 (1.8%) out of 219 from the nonendemic area, 10 (4.1%) out of 242 from the patients with chronic liver diseases were positive.165 individuals in the endemic region were typed for the promotor region by the method of PCR, and the genotype frequency was as follows ; 0.855 for TNF1/TNF1, 0.115 for TNF1/TNF2 and 0.03 for TNF2/TNF2. The frequency of TNF2 allele in the endemic area in Sichuan was significantly higher (0.176) than those in Japan (0.0) and other schistosomiasis-nonendemic area like as Solomon Islands (0.0) (p<0.01).