STUDY ON LIVER CIRRHOSIS AND HEPATOCELLULAR CARCINOMA IN CHINA

为了了解中国血吸虫病流行地区HBV和HCV感染的血清流行病学，我们与华西医科大学和四川省寄生虫病研究所进行了合作研究。对来自中国6个普通人群的1268份血清样本进行了HBV和HCV标记物检测。我们对中国四川地区104例肝硬化患者和138例肝细胞癌患者的血清样本进行了研究。519份日本血吸虫病流行区血清HBsAg阳性56份（10.8%），全部HBV标记物阴性164份（31.6%）。但第二代ELISA试剂盒未检测到抗- hcv。在3个老病区的530份样本中，HBsAg阳性79份（14.9%），全部HBV标记物阴性135份（25.5%）。抗- hcv阳性3例（0.57%）。来自2个非流行地区的219份样本中，HBsAg阳性18份（8.2%），所有HBV标志物均阴性70份（32.0%），Anti-HCV阳性36份（14.9%）。在慢性肝病中，在242例检查病例中，有170例（70.2%）检测到HBsAg。抗- hcv阳性36例（14.9%）。采用可溶性日本血吸虫卵抗原（SEA） ELISA法进行血吸虫病筛查。疫区519份标本中阳性339份（65.3%），老疫区530份标本中阳性87份（16.4%），非疫区219份标本中阳性4份（1.8%），慢性肝病患者242份标本中阳性10份（4.1%）。用PCR方法对疫区165例个体进行启动子区分型，分型频率为：TNF1/TNF1为0.855，TNF1/TNF2为0.115，TNF2/TNF2为0.03。四川血吸虫病流行区TNF2等位基因频率（0.176）显著高于日本（0.0）和所罗门群岛（0.0）等血吸虫病非流行区（p<0.01）。

To estimate the seroepidemiology of HBV and HCV infection in China where schistosomiasis japonica was endemic, we performed the collaborative studies with West China University of Medical Sciences and Sichuan Institute of Parasitic Disease.A total of 1,268 serum samples from six general populations in China were tested for HBV and HCV markers. And we have studied 104 serum samples from the patients with liver cirrhosis and 138 samples with hepatocellular carcinoma in Sichuan, China.Among 519 serum samples from the endemic areas of schistosomiasis japonica, 56 (10.8%) were positive for HBsAg, and 164 (31.6%) were negative for all HBV markers. But Anti-HCV was not detected with a second-generation ELISA kit. Among 530 samples from three old endemic area, 79 (14.9%) were positive for HBsAg, and 135 (25.5%) were negative for all HBV markers. And 3 (0.57%) were positive for Anti-HCV.Among 219 samples from two nonendemic area, 18 (8.2%) were positive for HBsAg, 70 (32.0%) were negative for all HBV markers, and 36 (14.9%) were positive for Anti-HCV.In chronic liver diseases, HBsAg was detected in 170 cases (70.2%) from 242 examined cases. Anti-HCV was positive in 36 cases (14.9%).ELISA using soluble Schistosoma japonicum eggs antigen (SEA) was used for the screening of schistosomiasis. 339 (65.3%) out of 519 samples from the endemic area, 87 (16.4%) out of 530 from the old endemic area, 4 (1.8%) out of 219 from the nonendemic area, 10 (4.1%) out of 242 from the patients with chronic liver diseases were positive.165 individuals in the endemic region were typed for the promotor region by the method of PCR, and the genotype frequency was as follows ; 0.855 for TNF1/TNF1, 0.115 for TNF1/TNF2 and 0.03 for TNF2/TNF2. The frequency of TNF2 allele in the endemic area in Sichuan was significantly higher (0.176) than those in Japan (0.0) and other schistosomiasis-nonendemic area like as Solomon Islands (0.0) (p<0.01).