Study on the molecules responsible for implantation window

为了建立胚胎着床及随后的分化、增殖和识别子宫内胚胎附着的控制方法，应用DNA微阵列技术检测了同一受者将受精卵和8-细胞期胚胎分别移植到输卵管右侧和左侧的子宫组织中启动着床窗口的基因的表达模式。结果，在植入窗打开时，发现了一个下调和两个上调的基因。利用实时定量聚合酶链式反应的方法，进一步分析了这些可能与着床窗口相关的基因在着床前发育和着床过程中的表达谱。Sprr2f的表达在0.5dPC时最高，随着着床前发育和着床过程中的表达逐渐减弱。而在3.5dpc，Sprr2f在卵巢组织中有较强的表达。As1在4.5dpc的子宫和3.5dpc的卵巢中高表达。在围着床期的子宫和卵巢组织中，需要进一步分析这些分子作为调控着床窗口的候选基因。

To develop a control method for embryonic implantation and subsequent differentiation and proliferation as well as recognition of embryonic attachment in uterus, expression pattern of genes responsible for initiation of implantation window in uterine tissues from an identical recipient which transferred fertilized oocytes at pronucleus stage and embryos at 8-cell stage into right and left side of the oviduct, respectively, was examined by using a DNA microarray. As a result, at the open of implantation window, one down-regulated and two up-regulated genes were identified. These presumably responsible genes for window of implantation were further analyzed the expression profile during the course of preimplantation development and implantation by a real time PCR method. Expression of Sprr2f was highest on 0.5 dpc and gradually decreased with preimplantation development and during implantation. However, Sprr2f strongly expressed in ovarian tissues on 3.5 dpc. Ass1 highly expressed in uterus on 4.5 dpc and in ovary on 3.5 dpc. Further analysis of these molecules as candidates of genes responsible for regulation of implantation window is required in uterine and ovarian tissues at peri-implantation period.