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Genetic studies of Dipteyocarpaceae in Malaysian rainforests

马来西亚雨林中龙脑香科的遗传研究

基本信息

批准号：
06044172
负责人：
YAMAZAKI Tsuneyuki
金额：
$ 4.99万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044172/
关键词：
gene diversity tropical forest plant evolution population genetics genetic diversity PCR 集団構造 Gap C

项目摘要

Our main objective of the project is to obtain basic data on genetic and species diversity, collectively called biodiversity, of plants and design schemes to preserve them efficiently. we focused at the study of genetic and species diversity in three different forest populations ; inland trees of monsoon forests in Thailand, pine trees in Hokkaido, Japan, and Shorea of Malasian tropical rain forests Species to be studied in Japanese populations are several species of pinetrees and Japanese cedar. Plant populations in target in Thailand include many natural populations available throughout the country as well as at least one man-made forest, so called Mr.Wibul forest. Many species of inland trees including Dipterocarpus group, in addition to over 20 spesies of manglobe trees are covered in this study. After purifying DNA from leaf samples of various trees DNA sequencing were done using an spacer region of tRNA of chloroplast and GAP-C genes of nucleus. No genetic variatio was found in any of dominat four species of inland trees in Thailand, in contrast to the high genetic variability found in both Japanese and Malasian populations. The amount of gene diversity found in Japanese and Malasian populations were from 0.002 to 0.01 depending on the genes and populations investigated. This striking differences in genetic variations between Thailand monsoon forests and other populations (Malasian and Japanese) may raise serious problem in the method of maintenance and sustainable use of tropical forests.

我们项目的主要目标是获取植物遗传和物种多样性的基本数据，并设计有效保护它们的方案。我们集中研究了三个不同森林种群的遗传和物种多样性；泰国季风林的内陆树木、日本北海道的松树和马来西亚热带雨林的松木。在日本种群中研究的物种有几种松树和日本雪松。泰国目标地区的植物种群包括全国各地的许多自然种群，以及至少一个人造森林，即所谓的Mr.Wibul森林。本研究涵盖了陆生乔木包括梁木属在内的许多种，以及20多种红木。对不同树种叶片样品进行DNA纯化后，利用叶绿体tRNA间隔区和细胞核GAP-C基因进行DNA测序。与在日本和马来西亚种群中发现的高遗传变异性相比，在泰国的主要四种内陆树木中没有发现任何遗传变异。日本和马来西亚人群的基因多样性在0.002 ~ 0.01之间，根据基因和群体的不同而不同。泰国季风林与其他种群（马来西亚和日本）在遗传变异方面的这种显著差异可能会在热带森林的维护和可持续利用方法方面引起严重问题。