Genetic studies of Dipteyocarpaceae in Malaysian rainforests

我们这个项目的主要目标是获得有关植物遗传和物种多样性(统称为生物多样性)的基本数据，并设计有效保护它们的方案。我们重点研究了泰国季风林的内陆树木、日本北海道的松树和马拉西亚热带雨林的Shorea三个不同森林种群的遗传多样性和物种多样性。泰国的目标植物种群包括全国各地的许多自然种群，以及至少一片人工林，也就是所谓的威布尔先生森林。除了20多个种的万寿菊外，还有包括龙脑果在内的许多内陆树种。从不同树种的叶片样品中提纯DNA后，利用叶绿体tRNA间隔区和细胞核GAP-C基因进行DNA测序。在泰国的四种主要内陆树种中，没有发现任何一种存在遗传变异，这与日本和马拉西亚种群中发现的高度遗传变异形成了鲜明对比。根据所研究的基因和种群的不同，在日本和马拉西亚种群中发现的基因多样性的数量从0.002到0.01%。泰国季风林与其他种群(马拉西亚人和日本人)之间遗传变异的显著差异可能会给热带森林的维护和可持续利用带来严重的问题。

Our main objective of the project is to obtain basic data on genetic and species diversity, collectively called biodiversity, of plants and design schemes to preserve them efficiently. we focused at the study of genetic and species diversity in three different forest populations ; inland trees of monsoon forests in Thailand, pine trees in Hokkaido, Japan, and Shorea of Malasian tropical rain forests Species to be studied in Japanese populations are several species of pinetrees and Japanese cedar. Plant populations in target in Thailand include many natural populations available throughout the country as well as at least one man-made forest, so called Mr.Wibul forest. Many species of inland trees including Dipterocarpus group, in addition to over 20 spesies of manglobe trees are covered in this study. After purifying DNA from leaf samples of various trees DNA sequencing were done using an spacer region of tRNA of chloroplast and GAP-C genes of nucleus. No genetic variatio was found in any of dominat four species of inland trees in Thailand, in contrast to the high genetic variability found in both Japanese and Malasian populations. The amount of gene diversity found in Japanese and Malasian populations were from 0.002 to 0.01 depending on the genes and populations investigated. This striking differences in genetic variations between Thailand monsoon forests and other populations (Malasian and Japanese) may raise serious problem in the method of maintenance and sustainable use of tropical forests.