权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Symbiotic Evolution between Dipterocarpaceae, Fungi & Insects

龙脑香科、真菌之间的共生进化

基本信息

批准号：
10041172
负责人：
YAMAZAKI Tsuneyuki
金额：
$ 3.78万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10041172/
关键词：
symbiosis Dipterocarpus fungi Drosophila

项目摘要

In 1998 and 1999 several expedition trips were conducted to Malaysia, Thailand, and Srilanka : to Kinabaru area, Malaysia in July, 1998 ; to Dipterocarp forest in FRIM, Malaysia in September, 1998 ; to Semengo National Park, Saba, Malaysia in September, 1998 ; to a natural forest near Bangkok in January 1999 ; to natural forests in Chiangmai and Chiangrai in January 1999 ; to Paso, Kuba, and Semengo in Thailand in March 1999 ; to Narachiwat in southern Thailand in March 1999 ; to several forests in Srilanka in January 2000. Using the materials obtained during the above expeditions we tried to determine the specificity of the symbiosis between Dipterocarp and fungi, and between fungi and Drosophila by identifying the species name of these organisms by the method of DNA sequencing. Several chloroplast DNA regions (matK, intergenic regions between trn L-trn F, intron of trn L), and a nuclear gene, gapC were used for Dipterocarpus, and a part of rRNA gene was used for the analysis of fungi. ITS region and amylase were tried for Drosophila. We could establish a good reliable molecular phylogenetical tree of Dipterocarp species using these sequencing data. As for the tree of fungi published trees of rRNA were improved by adding our sequencing data. In most cases no clear specificities were found between symbiotic Dipterocarp species and fungi. The accumulation of more data is essential for obtaining general features of symbiosis. No data are available yet for the relationship between fungi and Drosophila since we are still trying to identify the name of Drosophila.

1998年和1999年，对马来西亚、泰国和斯里兰卡进行了几次考察旅行：1998年7月到马来西亚Kinabaru地区; 1998年9月到马来西亚FRIM的龙脑香林; 1998年9月到马来西亚Saba的Semengo国家公园; 1999年1月到曼谷附近的一个自然森林; 1999年1月到清迈和清莱的天然森林; 1999年3月到泰国的帕索、库巴和塞门戈; 1999年3月到泰国南部的那拉奇瓦; 2000年1月到斯里兰卡的几个森林。利用在上述考察中获得的材料，我们试图通过DNA测序的方法确定这些生物的种名，来确定龙脑香果和真菌之间以及真菌和果蝇之间的共生的特异性。几个叶绿体DNA区域（matK，trn L-trn F之间的基因间隔区，trn L的内含子）和一个核基因gapC用于龙脑香属，并且一部分rRNA基因用于真菌的分析。对果蝇的ITS区和淀粉酶进行了研究。利用这些测序数据可以建立可靠的龙脑香属植物分子遗传树。对于真菌树，通过添加我们的测序数据改进了已发表的rRNA树。在大多数情况下，没有明确的特异性之间发现共生的龙脑香属物种和真菌。积累更多的数据对于获得共生的一般特征是必不可少的。真菌和果蝇之间的关系还没有数据，因为我们仍然在试图确定果蝇的名称。