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Gene library planning based on the salivary gland chromosome map of Drosophila melanogaster

基于果蝇唾液腺染色体图谱的基因库规划

基本信息

批准号：
02554025
负责人：
YAMAZAKI Tsuneyuki
金额：
$ 9.41万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

The purpose of this study is to establish a random clone library of Drosophila melanogaster which are mapped on the salivary gland chromosome and construct a data bank system based on them. we isolated about 800 clones randomly from the lambda phage genomic library of Drosophila melanogaster and constructed a phage stock library and extracted DNA samples from each clone. We mapped these clones on the salivary gland chromosome by in situ hybridization technique. Up to date, 286 clones are mapped and the rest of them are now under progress. 190 clones are mapped on the single sites on the chromosomes and the rest of 96 (33.6%) are mapped on the multiple locations. These clones are considered to contain repetitive sequences or the movable genetic elements. Among these clones which were mapped on the single sites, 31 were mapped on the X, 40 were mapped on the 2L, 55 were mapped on the 2R, 35 were mapped on the 3L and 37 were mapped on the 3R. This result indicates that clones were chosen randomly from the genome. Assuming the Poisson distribution the mean frequency of clones having a repetitive sequence is estimated to be 0.409.On the other hand, we collected DNA clones of Drosophila melanogaster which have been mapped on the salivary gland chromosomes from the laboratories of all over the world. We so far obtained about 50 clones. Further more we are to get P1 phage library clones from Dr. G. Rubin of Univ. of California. After we establish the clone bank of these collections, we will start service for distribute these clones on request.

本研究的目的是建立果蝇唾腺染色体随机克隆文库，并以此为基础构建数据库系统。我们从黑腹果蝇的λ噬菌体基因组文库中随机分离了约800个克隆，构建了噬菌体储备文库，并从每个克隆中提取DNA样品。我们通过原位杂交技术将这些克隆定位在唾液腺染色体上。到目前为止，已绘制了286个克隆，其余的正在绘制中。190个克隆定位在染色体上的单个位点上，其余96个（33.6%）定位在多个位点上。这些克隆被认为含有重复序列或可移动的遗传元件。在单位点定位的克隆中，31个定位在X染色体上，40个定位在2L染色体上，55个定位在2R染色体上，35个定位在3L染色体上，37个定位在3R染色体上。该结果表明克隆是从基因组中随机选择的。在Poisson分布下，具有重复序列的克隆的平均频率为0.409。另一方面，我们收集了来自世界各地的果蝇唾液腺染色体上的DNA克隆。到目前为止，我们获得了大约50个克隆。进一步从Dr. G.加州大学的鲁宾。在我们建立了这些集合的克隆库之后，我们将根据请求启动分发这些克隆的服务。