Cell biological studies of hard tissue using three dimensional morphometry.

使用三维形态测定法对硬组织进行细胞生物学研究。

基本信息

批准号：
06404063
负责人：
OZAWA Hidehiro
金额：
$ 16.64万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

This study aimed to examine morphological changes caused by bone metabolism using a 3-dimensional histomorphometry and cytomorphometry . The results obtained were as follows :1)Examined physiologic and pathologic changes of bone, e.g., aging of mandible and osteoporosis with CLSM and storing the views as digitized microscopic images . 2)The stored images were used for developing a madible morphometric image analysis and data management software, which enabled a semi-automated analysis with a Luzex-F for the following parameters : Bone surface ; Osteoid surface ; Osteoblast surface ; Eroded surface ; Psteoclast surface ; Bonevolume ; Osteoid volume ; Total tissue volume ; Fibrosis volume ; Single labeled surface ; Double labeled surface ; Label thickness ; Osteoid thickness ; Wall thickness ; Quiescent surface ; Low mineralizing bone volume . 3)Store fluorescence images obtained by CLSM observation of tetracyclin-and calcein-labeled undecalcified specimens, analyze 2-dimensional images, then use the 2-D images to reconstitute into 3-Dimages of the specimens by Micro Voxel. The results showed that the procedure enables the reconstitution of 3-D images of tetracyclin-and calcein-labeled volume up to a 100 um depth from the sample surface. 4)Theuse of Luzex-III,a surface modeling software, succeeded in simultaneously reconstitute the 3-D spatial relationship between osteoclasts, osteoblasts and the bone surface. The above-described procedures were used for : i)a morphological and immunohistochemical study on the effect of aging on rat mandibular bone ; ii)a morphological and immunohistochemical study on rat alveolar bone ; iii)study on the differential effect of PTH on bone metabolism according to the anatomical regions of bone ; iv)ultrastructural study on bone resorption in ovarectomized rats.

这项研究旨在检查使用三维的组织形态法和细胞形态计量法引起的骨代谢引起的形态变化。所获得的结果如下：1）检查了骨骼的生理和病理学变化，例如，使用CLSM衰老和骨质疏松症的老化，并将视图作为数字化显微镜图像存储。 2）存储的图像用于开发可爱的形态图像分析和数据管理软件，该软件能够通过以下参数进行LUZEX-F进行半自动分析：骨表面；骨质表面；成骨细胞表面；侵蚀的表面； PSTEOCLAST表面；骨伏；骨质体积；总组织体积；纤维化体积；单个标记的表面；双标记表面；标签厚度；骨质厚度；壁厚；静止的表面；低矿化骨体积。 3）存储通过CLSM观察四环素和钙软蛋白标记的未排放标本获得的荧光图像，分析2维图像，然后使用2-D图像通过微体素将2-D图像重新构造成三维标本。结果表明，该过程可以重建四环素和加拿大钙蛋白标记的体积的3-D图像，高于样品表面100 um。 4）表面建模软件Luzex-III的用途同时重建了破骨细胞，成骨细胞和骨表面之间的3-D空间关系。上述程序用于：i）关于衰老对大鼠下颌骨影响的形态和免疫组织化学研究； ii）关于大鼠牙槽骨的形态和免疫组织化学研究； iii）研究PTH对骨骼代谢的差异作用，根据骨的解剖区域； iv）卵巢切除大鼠骨吸收的超微结构研究。