Histological cna histochenmical studies on the bone metabolism using g confocal laser scanning microscope.

为了将激光共聚焦扫描显微镜(CLSM)用于骨代谢的研究，提出了将骨染色技术应用于骨组织块的基本方法，并将CLSM同时应用于包括三维观察在内的组织形态计量学方法。上述结果为激光共聚焦显微镜在骨代谢研究中的应用奠定了基础。1)大鼠下颌软骨和牙槽骨衰老变化的组织化学研究。2)甲状旁腺素治疗后骨代谢的组织形态计量学和组织化学研究。3)去卵巢大鼠骨小梁减少规律的组织学研究。4)24R.25(OH)_2D_3对实验性大鼠肾性骨畸变影响的骨组织形态计量学研究。5)利用大鼠顶骨缺损骨组织化学研究转化生长因子-β的成骨活性。6)硫酸乙酰肝素蛋白多糖在骨骼组织中定位的免疫组织化学研究。7)CD44在破骨细胞和骨细胞中的免疫组织化学定位。8)M-CSF.给药后Mac-2在OP/OP小鼠股骨中定位的免疫组织化学研究。9)大鼠骨组织中CGRP阳性神经纤维的免疫组织化学定位。10)RGD肽对培养破骨细胞形态变化的组织化学研究。这些组织形态计量学和组织学结果表明了CLSM的有效性。同时，对CLSM的改进进行了三维定量分析。还指出了利用具有中等分辨率的光学显微镜和电子显微镜的激光共聚焦显微镜在细胞学分析水平上迅速发展的可能性。

In order to use confocal laser scanning microscope (CLSM) for the research on bone metabolism, basic methods which entailed the application of bone staining techniques on histological tissue blocks of bone, and concurrent application of CLSM to a histomorphometrical method including three dimensional observation was carried out. The results of the above served as a basis for the application of CLSM to the following research pertaining to bone metabolism.1)Histochemical studies on the aging changes of the rat mandibular chondyle and alveolar bone.2)Bone histomorphometrical and histochemical studies on bone metabolism after PTH administration.3)Histological studies on the decreasing pattern of bone trabecules using ovariectomized rat.4)Bone histomorphometrical studies on the effect of 24R.25(OH)_2D_3 to the experimental renal osteodistorophy of rats.5)Histochemical studies on the bone formation activities of TGF-b using bone defects of rat parietal bone. 6)Immunohistochemical studies of the location f heparan sulfate proteoglycan in skeletal tissues.7)Immunohistochemical location of CD44 in osteoclast and osteocytes.8)Immunohistochemical studies on the location of Mac-2 in the femur of OP/OP mice after administration of M-CSF.9)Immunohistochemical locations of CGRP positive nerve fibers in the rat bone tissues.10)Histochemical studies on the morphological changes of cultures osteoclasts after the administrations of RGD peptide. These histomorphometrical and histological results made clear the usefulness of CLSM.At the same time, the added improvements of CLSM have made three dimensional quantitative analysis. The possibility of rapid development at the level of cytological analysis through use of CLSM which possesses the intermediate resolution of light and electron microscopy is also indicates.