Development of microscopic image processing system.

显微图像处理系统的开发。

基本信息

批准号：
06557093
负责人：
OZAWA Hidehiro
金额：
$ 9.66万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06557093/
关键词：
Microscopic image processing system Image database Image analysis DTP High-resolution printer CLSM Light microscope Electron microscope 骨形態計測顎骨加齢卵巣摘出 PTH

项目摘要

The objective of this study was to develop software package for the managment of cumulating microscopic image dataflow that enables temporary storage, archiving, image database retrieval and management, image analysis, and report layout and output with a DTP subroutine and high-resolution printer. The package optimizes the research task flow and image data sharing. The digitized images are free from color fading or other degradations in the image quality intrinsic to photographs. The hardwares consisted in a CLSM,a light microscope, an EM902 electron microscope connected through an interface to a computer system with a DTP software and a image analysis software. Microscopic images were either directly captured with a high-resolution CCD camera and stored or transferred from the CLSM or electron microscopes storage device using MO disks as transfer media. An FR-3000 film recorder was used as output device. The system thus developped was used for : i) Application of CLSM in the study of the ultrastructure of teeth ; ii) a morphological and immunohistochemical study on the effect of aging on rat mandibular bone ; iii) a histological and cytological study on physiological aging and tissue response to orthodontic force through aging in rat alveolar bone ; iv) cytological study of alveolar bone resorption during experimentally-induced periodontitis ; iv) Immunohistochemical and cytochemical localization of H+-ATPase and carbonate dehydratase in rat incisor ameloblasts.

这项研究的目的是为累积显微图像数据流进行管理软件包，该软件包可以使用DTP子例程和高分辨率打印机，使临时存储，归档，图像数据库检索和管理，图像分析以及报告布局和输出。软件包优化了研究任务流和图像数据共享。数字化的图像没有颜色褪色或图像质量固有的固有质量的颜色褪色。硬件由CLSM，光学显微镜，EM902电子显微镜组成，该电子显微镜通过接口连接到具有DTP软件和图像分析软件的计算机系统。使用高分辨率CCD摄像头直接捕获显微镜图像，然后使用MO磁盘作为传输介质存储或转移从CLSM或电子显微镜存储设备传输。 FR-3000胶片记录器用作输出设备。因此开发的系统用于：i）CLSM在牙齿的超微结构研究中的应用； ii）一项关于衰老对大鼠下颌骨影响的形态和免疫组织化学研究； iii）一项关于通过大鼠肺泡骨老化的生理衰老和组织对正畸力的组织反应的组织学和细胞学研究； iv）在实验诱导的牙周炎期间肺泡骨吸收的细胞学研究； iv）H+ - ATPase和碳酸盐脱氢酶在大鼠牙入牙入牙片中的免疫组织化学和细胞化学定位。