Development of microscopic image processing system.

这项研究的目的是开发用于管理累积的显微图像数据流的软件包，该软件包能够使用DTP子例程和高分辨率打印机实现临时存储、存档、图像数据库检索和管理、图像分析以及报告布局和输出。该包优化了研究任务流程和图像数据共享。数字化图像没有颜色褪色或照片固有的图像质量的其他降级。硬件包括激光共聚焦显微镜、光学显微镜、EM902电子显微镜，通过接口连接到带有DTP软件和图像分析软件的计算机系统。显微图像要么用高分辨率的CCD摄像机直接捕获并从CLSM存储或传输，要么使用MO盘作为传输介质的电子显微镜存储设备。输出设备为FR-3000型录像机。该系统用于：i)激光共聚焦显微镜在牙齿超微结构研究中的应用；ii)衰老对大鼠下颌骨的影响的形态和免疫组织化学研究；iii)大鼠牙槽骨的生理性衰老和通过衰老对正畸力的组织反应的组织学和细胞学研究；iv)实验性牙周炎过程中牙槽骨吸收的细胞学研究；iv)大鼠切牙或成釉细胞中H-ATPase和碳酸盐脱水酶的免疫组织化学和细胞化学定位。

The objective of this study was to develop software package for the managment of cumulating microscopic image dataflow that enables temporary storage, archiving, image database retrieval and management, image analysis, and report layout and output with a DTP subroutine and high-resolution printer. The package optimizes the research task flow and image data sharing. The digitized images are free from color fading or other degradations in the image quality intrinsic to photographs. The hardwares consisted in a CLSM,a light microscope, an EM902 electron microscope connected through an interface to a computer system with a DTP software and a image analysis software. Microscopic images were either directly captured with a high-resolution CCD camera and stored or transferred from the CLSM or electron microscopes storage device using MO disks as transfer media. An FR-3000 film recorder was used as output device. The system thus developped was used for : i) Application of CLSM in the study of the ultrastructure of teeth ; ii) a morphological and immunohistochemical study on the effect of aging on rat mandibular bone ; iii) a histological and cytological study on physiological aging and tissue response to orthodontic force through aging in rat alveolar bone ; iv) cytological study of alveolar bone resorption during experimentally-induced periodontitis ; iv) Immunohistochemical and cytochemical localization of H+-ATPase and carbonate dehydratase in rat incisor ameloblasts.