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Development of microscopic image processing system.

显微图像处理系统的开发。

基本信息

批准号：
06557093
负责人：
OZAWA Hidehiro
金额：
$ 9.66万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06557093/
关键词：
Microscopic image processing system Image database Image analysis DTP High-resolution printer CLSM Light microscope Electron microscope 骨形態計測顎骨加齢卵巣摘出 PTH

项目摘要

The objective of this study was to develop software package for the managment of cumulating microscopic image dataflow that enables temporary storage, archiving, image database retrieval and management, image analysis, and report layout and output with a DTP subroutine and high-resolution printer. The package optimizes the research task flow and image data sharing. The digitized images are free from color fading or other degradations in the image quality intrinsic to photographs. The hardwares consisted in a CLSM,a light microscope, an EM902 electron microscope connected through an interface to a computer system with a DTP software and a image analysis software. Microscopic images were either directly captured with a high-resolution CCD camera and stored or transferred from the CLSM or electron microscopes storage device using MO disks as transfer media. An FR-3000 film recorder was used as output device. The system thus developped was used for : i) Application of CLSM in the study of the ultrastructure of teeth ; ii) a morphological and immunohistochemical study on the effect of aging on rat mandibular bone ; iii) a histological and cytological study on physiological aging and tissue response to orthodontic force through aging in rat alveolar bone ; iv) cytological study of alveolar bone resorption during experimentally-induced periodontitis ; iv) Immunohistochemical and cytochemical localization of H+-ATPase and carbonate dehydratase in rat incisor ameloblasts.

本研究的目的是开发用于管理累积显微图像数据流的软件包，该软件包可以通过DTP子程序和高分辨率打印机实现临时存储，存档，图像数据库检索和管理，图像分析以及报告布局和输出。该软件包优化了研究任务流程和图像数据共享。数字化图像不受照片固有图像质量的颜色褪色或其他退化的影响。硬件包括一台CLSM，一台光学显微镜，一台EM902电子显微镜，通过接口连接到带有DTP软件和图像分析软件的计算机系统。显微图像由高分辨率CCD相机直接捕获，并以MO盘作为传输介质从CLSM或电子显微镜存储装置中存储或传输。用一台FR-3000胶片记录仪作为输出设备。该系统主要用于：1)CLSM在牙齿超微结构研究中的应用；Ii)衰老对大鼠下颌骨影响的形态学和免疫组织化学研究；Iii)大鼠牙槽骨生理老化及组织对正畸力老化反应的组织学和细胞学研究；Iv)实验性牙周炎牙槽骨吸收的细胞学研究；iv)大鼠门牙成釉细胞中H+- atp酶和碳酸盐脱水酶的免疫组织化学和细胞化学定位。