Development of microscopic image processing system.

本研究的目的是开发用于管理累积显微图像的软件包，该软件包能够临时存储、归档、图像数据库检索和管理、图像分析以及具有DTP子程序和高分辨率打印机的报告布局和输出。该软件包优化了研究任务流程和图像数据共享。数字化的图像没有褪色或照片固有的图像质量的其他退化。硬件包括CLSM，光学显微镜，EM 902电子显微镜通过接口连接到具有DTP软件和图像分析软件的计算机系统。显微图像直接捕获的高分辨率CCD相机和存储或从CLSM或电子显微镜存储设备使用MO磁盘作为传输介质传输。使用FR-3000胶片记录仪作为输出装置。本系统用于：（1）CLSM在牙齿超微结构研究中的应用;（2）衰老对大鼠下颌骨影响的形态学和免疫组化研究;（3）生理衰老和正畸力对大鼠牙槽骨组织反应的组织学和细胞学研究; iv）实验性牙周炎时牙槽骨吸收的细胞学研究; iv）大鼠切牙成釉细胞中H+-ATP酶和碳酸酯酶的免疫组织化学和细胞化学定位。

The objective of this study was to develop software package for the managment of cumulating microscopic image dataflow that enables temporary storage, archiving, image database retrieval and management, image analysis, and report layout and output with a DTP subroutine and high-resolution printer. The package optimizes the research task flow and image data sharing. The digitized images are free from color fading or other degradations in the image quality intrinsic to photographs. The hardwares consisted in a CLSM,a light microscope, an EM902 electron microscope connected through an interface to a computer system with a DTP software and a image analysis software. Microscopic images were either directly captured with a high-resolution CCD camera and stored or transferred from the CLSM or electron microscopes storage device using MO disks as transfer media. An FR-3000 film recorder was used as output device. The system thus developped was used for : i) Application of CLSM in the study of the ultrastructure of teeth ; ii) a morphological and immunohistochemical study on the effect of aging on rat mandibular bone ; iii) a histological and cytological study on physiological aging and tissue response to orthodontic force through aging in rat alveolar bone ; iv) cytological study of alveolar bone resorption during experimentally-induced periodontitis ; iv) Immunohistochemical and cytochemical localization of H+-ATPase and carbonate dehydratase in rat incisor ameloblasts.