Analysis of molecular mechanisms in bone formation and regeneration

I.骨基质形成中钙化的分子机制：为了鉴定诱导核形成的因子，我们用从培养的MLO-A5细胞中分离的基质囊泡制备了单克隆抗体，并阐明了抗原定位于细胞外基质中的纤维之间。此外，为了评价镁在骨钙化中的作用，我们测定了缺镁大鼠的骨质量，特别是矿化。镁的丢失使钙含量增加，羟基磷灰石结晶不规则。这些结果表明，镁的损失抑制了基质囊泡中羟基磷灰石的结晶。骨代谢偶联机制：骨保护素（OPG）缺陷小鼠骨形成和骨吸收促进。双膦酸盐是一种潜在的骨吸收抑制剂，可减少骨形成和骨吸收。骨形态发生蛋白诱导的骨形成在OPG缺陷小鼠中与对照组无任何差异。 关于我们 t在野生小鼠中。这些结果提示破骨细胞激活或释放的偶联因子可能参与了随后的骨形成。我们研究了脂多糖和白细胞介素（IL）-1诱导破骨细胞生成的机制。这些因子通过MyD 88和Ca/PKC-MEK/ERK途径诱导成骨细胞表达RANKL。骨再生与重建模型：大鼠牙髓皮下移植后形成具有骨样或牙骨质样特征的矿化硬组织，提示牙髓具有自身硬组织形成的能力。激光照射诱导成骨作用是通过激活成牙本质细胞和抑制成骨细胞的骨吸收而实现的。在前列腺素E EP 4受体激动剂的研究中，成牙本质细胞中存在基因表达，骨髓中脂肪细胞减少，成骨细胞增加是导致成骨活性的原因。少

I. Molecular mechanisms of calcification in bone matrix formation : To identify the factor induce formation of the nuclear, we make monoclonal anti-body using matrix vesicle isolated from MLO-A5 cell in culture and clarified that the antigen localizes among the fibrae in extracellular matrix. Moreover, to estimate the effects of Mg in bone calcification, we determined the bone qualities, especially mineralization in Mg deficient rat. Loss of Mg was increased the value of Ca and had irregular crystal of hydroxyapatite in the rat. These findings indicate that the loss of Mg inhibits the crystallization of hydroxyapatite in matrix vesicle.II. Coupling mechanisms of bone metabolism : Bone formation and bone resorption were promoted in osteoprotegerin (OPG)-deficient mice. Bisphosphonate, a potential inhibitor of bone resorption, reduced both bone formation and bone resorption. Bone formation induced by bone morphogenetic protein in OPG-deficient mice did not exhibit any difference from tha … More t in wild mice. These results suggest that coupling factors activated or released by osteoclasts may be involved in the subsequent bone formation. We investigated the mechanism of osteoclastogenesis induced by lipopolysaccharide and interleukin (IL)-1. These factors induced the expression of RANKL in osteoblastic cells through MyD 88 and Ca/PKC-MEK/ERK pathways.III. Bone regeneration and reconstruction model : The subcutaneously transplanted rat dental pulp formed mineralized hard tissue possessing bone- or cementum-like characteristics, suggesting that dental pulp possesses the ability to hard tissue formation by itself. Bone formation induced laser light irradiation was shown to be caused by the odontoblast activation and the inhibition of osteoclastic bone resorption. In the study of prostaglandin E EP4 receptor agonist, the gene expression existed in odontoblast, and the bone formation activity was resulted from the decrease of adipocyte and the increase of osteoblast in bone marrow. Less