Reasearch for control system of bone morphogy adapted to ageing or environment

适应衰老或环境的骨形态控制系统研究

基本信息

批准号：
11307038
负责人：
OZAWA Hidehiro
金额：
$ 26.11万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11307038/
关键词：
ageing Bone cartilage fine strucutre histochemistry calcification micro CT

项目摘要

Bone morphology is maintained by the balance among osteocytes, osteoblasts and osteoclasts, or interaction of surrounding tissues including cartilage, vessels, nerve, and tendon. However, the mechnisms by which bone morphology is diverse accodrding to the alteration of age, environment, and species remain unclear. In order to elucidate control system of bone morphology, we analyzed(1) formation and mantenance mechanisms of calcified bone matrix, and (2) morphological alteration of bones, accoding to aging, environment, and species.In osteoid, meshwork of proteoglycans are oberved among collagen fibrils. Calcium was localized at the sites of proteoglycans, while phosphorus was often mapped to collagen fibrils. These structures may preserve each minerals, and are thought to inhibit the increase of local calciumXpohosphorus product, leading to the maintenence of uncalcified state. In contrast, calcium and phosphorus were co-localized at the calcified nodules, while sizes of proteoglycan became smaller there. The co-localization of calcium and phosphorus following such fragmentation of proteoglycan was thought to play an important role in undergoing calcifications.Regarding the second topics, bone morphology reaseably changes according to the metabolic or anabolic actions of various factors including estrogen, parathyroid hormone, bone morphogenetic protein, and tensile stress. Espepcially, it was revealed that osteoblastic differntiation promotes accoding to the tensile stress, mediated by increase of BMP-4 expression in preosoteoblasts and periosteoal cells.In order to examine bone diversity among spepcies, teeth and surroudning bones of gold fish were evaluated by micro CT.In micro CT analysis, fragile organs can be easily investigated without any specific prepartion. Thus, characteristics of teeht and surrouning bones of gold fish were clarified.

骨形态是由骨细胞，成骨细胞和破骨细胞之间的平衡来维持的，或周围组织的相互作用，包括软骨，血管，神经和肌腱。然而，骨形态学的机甲对年龄，环境和物种的改变的多样化仍然不清楚。为了阐明骨形态的控制系统，我们分析了（1）（1）钙化骨基质的形成和辐射机制，以及（2）骨骼形态学改变，对衰老，环境和物种的指导，骨质骨化，蛋白聚糖的网状作品。钙位于蛋白聚糖的部位，而磷通常映射到胶原蛋白原纤维。这些结构可以保留每种矿物质，并被认为可以抑制局部钙的增加，从而导致未含有未含有的状态的维持。相反，钙和磷在钙化的结节上共定位，而蛋白聚糖的尺寸在那里变小。蛋白聚糖碎片化后，钙和磷的共定位被认为在进行钙化中起着重要作用。根据雌激素，雌激素，甲状旁腺激素，骨形成蛋白和刺激性蛋白质和刺激性，骨形态可重复变化。尤其是在揭示的是，成骨细胞的不同促进了拉伸应力，这是通过在粒细胞细胞和骨膜细胞中的BMP-4表达增加来介导的，以检查牙齿，牙齿，牙齿之间的骨骼多样性，并通过Microro ct.in ext.in ct.in trabiire ct。因此，阐明了Teeht和牙骨的特征。