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Reasearch for control system of bone morphogy adapted to ageing or environment

适应衰老或环境的骨形态控制系统研究

基本信息

批准号：
11307038
负责人：
OZAWA Hidehiro
金额：
$ 26.11万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11307038/
关键词：
ageing Bone cartilage fine strucutre histochemistry calcification micro CT

项目摘要

Bone morphology is maintained by the balance among osteocytes, osteoblasts and osteoclasts, or interaction of surrounding tissues including cartilage, vessels, nerve, and tendon. However, the mechnisms by which bone morphology is diverse accodrding to the alteration of age, environment, and species remain unclear. In order to elucidate control system of bone morphology, we analyzed(1) formation and mantenance mechanisms of calcified bone matrix, and (2) morphological alteration of bones, accoding to aging, environment, and species.In osteoid, meshwork of proteoglycans are oberved among collagen fibrils. Calcium was localized at the sites of proteoglycans, while phosphorus was often mapped to collagen fibrils. These structures may preserve each minerals, and are thought to inhibit the increase of local calciumXpohosphorus product, leading to the maintenence of uncalcified state. In contrast, calcium and phosphorus were co-localized at the calcified nodules, while sizes of proteoglycan became smaller there. The co-localization of calcium and phosphorus following such fragmentation of proteoglycan was thought to play an important role in undergoing calcifications.Regarding the second topics, bone morphology reaseably changes according to the metabolic or anabolic actions of various factors including estrogen, parathyroid hormone, bone morphogenetic protein, and tensile stress. Espepcially, it was revealed that osteoblastic differntiation promotes accoding to the tensile stress, mediated by increase of BMP-4 expression in preosoteoblasts and periosteoal cells.In order to examine bone diversity among spepcies, teeth and surroudning bones of gold fish were evaluated by micro CT.In micro CT analysis, fragile organs can be easily investigated without any specific prepartion. Thus, characteristics of teeht and surrouning bones of gold fish were clarified.

骨形态是通过骨细胞、成骨细胞和破骨细胞之间的平衡，或周围组织（包括软骨、血管、神经和肌腱）的相互作用来维持的。然而，骨形态随年龄、环境和物种的变化而变化的机制仍不清楚。为了阐明骨形态的控制系统，我们分析了（1）钙化骨基质的形成和维持机制，以及（2）根据年龄、环境和物种的骨形态改变。在类骨质中，在胶原纤维之间观察到蛋白聚糖的网状结构。钙位于蛋白多糖位点，而磷通常定位于胶原纤维。这些结构可以保留每种矿物质，并被认为抑制局部钙X磷产物的增加，导致未钙化状态的维持。相反，钙和磷在钙化结节处共存，而蛋白多糖的尺寸则变小。蛋白多糖断裂后钙和磷的共定位被认为在钙化过程中发挥着重要作用。关于第二个主题，骨形态根据各种因素（包括雌激素、甲状旁腺激素、骨形态发生蛋白和拉伸应力）的代谢或合成代谢作用而发生明显变化。特别是，研究表明，前成骨细胞和骨膜细胞中 BMP-4 表达增加介导的拉伸应力会促进成骨细胞分化。为了检查物种之间的骨骼多样性，通过显微 CT 评估金鱼的牙齿和周围骨骼。在显微 CT 分析中，无需任何特殊准备即可轻松研究脆弱的器官。由此，金鱼牙齿和周围骨骼的特征得到了阐明。