Developmental construction of a flow type GPC/LALLS/Reversing Pulse Electric Birefringence apparatus for polymer solution study

聚合物溶液的溶液性质通常取决于溶质聚合物的形状、相对分子质量和分子量分布。在当前的科学发展研究中，我们设计并建造了一台流动式GPC/LALLS/RPEB(凝胶渗透色谱/小角度激光散射/反向脉冲电双折射)装置，用于同时测定溶质聚合物的重均相对分子质量、分子量分布、电光和流体力学性质。GPC/LALLS装置由两个色谱柱和一个泵浦系统以及串联的Tohso LS-8000型光散射光度计(东京Tohso公司)组成。RPEB装置是在我们实验室设计和建造的，针对目前的目的对已报道的设计原则进行了一些新的改进。该装置配备了脉冲电压为13KV、脉宽为5…的高压反向脉冲发生器超过00毫秒。设计和建造了一种流动式克尔池，其设计原理是节省池体积，防止样品溶液的再混合。这是通过使用两根直径0.2 mm、宽度0.5 mm的不锈钢细管作为电极和样品溶液的输入/输出来实现的。目前的克尔池的体积为2微升，非常适合流动型测量。离子强度控制系统没有安装在仪器上，因为它干扰了样品溶液的流动(混合)，使EB数据毫无用处。测量和仪器由两台微型计算机控制，一台用于GPC/LALLS系统，另一台用于RPEB系统。总体测量由样品注入GPC系统时的触发信号启动。Eb和PULSE信号通过瞬时转换器(东京Riken Densi Co.)数字化，然后通过自制接口传输到NEC PC-9821Bf-V8W微型计算机，数据存储在软盘上。较少

The solution property of the polymer solutions is normally dependent on the shapes, molecular weights, and molecular weight distributions of the solute polymers. In the present scientific developmental research, we designed and constructed a flow-type GPC/LALLS/RPEB (gel permeation chromatography/low-angle-laser-light scattering/reversing-pulse electric birefringence) apparatus for the simultaneous determination of the weight-average-molecular-weight, the molecular-weight-distribution, and the electro-optical and hydrodynamic properties of the solute polymers.GPC/LALLS apparatus consists of two columns and a pump systems and a Tohso LS-8000S light scattering photometer (Tohso Co., Tokyo) connected in series. RPEB apparatus was designed and constructed in our laboratory with some novel improvements of already reported design principles for the present purpose. The present RPEB apparatus is equipped a high voltage reversing pulse generator of the pulse voltage of 13 kv and its width of 5 … More 00 msec. A flow type Kerr cell was newly designed and constructed, of which design principle is the cell volume saving to prevent a remixing of the sample solution. This was attained by using two thin stainless steel pipes with a hole of 0.2 mm diameter and 0.5 mm width, which can serve as electrodes and in /out-lets of the sample solution. The present Kerr cell has a 2 microliter of volume and is very suitable for the flow type measurements. The ionic strength controlling system is not mounted to the apparatus, since it disturbs the sample solution flow (remixing) and makes the EB data useless. It is making improvements to filfull its function.The measurement and apparatus are controlled by two microcomputers ; the one is for GPC/LALLS and the other is for RPEB systems. The over all measurement is initiated by a trigger signal on the sample injection to the GPC system. Both EB and pulse signals are digitized through a transient converter (Riken Densi Co., Tokyo) and then transferred to an NEC PC-9821 Bf-V8W microcomputer via a homemade interface, and the data are stored on a floppy disk. Less