A membrane protein gp64 inhibits development of Polysphondylium

膜蛋白 gp64 抑制多毛藻的发育

• 批准号: 12640643
• 负责人: OCHIAI Hiroshi
• 金额: $ 2.5万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640643/
• 关键词: cellular slime mold; Polysphondylium; membrane protein; stalk differentiation; ecmB; gp64; Polysphondylium; 細胞性粘歯; 細胞膜タンパク質; Polysphond; ylium; 分枝; 形質転換

To examine the function of the membrane protein gp64 of Polysphondylium, a over expresser of gp64 was examined. In the light, when the wild-type and the overexpresser, both the strains developed into normal fruiting bodies. In the dark, the wild type developed lately but became fruiting bodies at 48 hr. In contrast the overexpresser the almost cells did not stream together, except migrating cylindrical slugs.

为了检测多软骨病的膜蛋白gp64的功能，检测了gp64的过表达。在光照条件下，野生型和超表达株均发育成正常的子实体。在黑暗中，野生型开发晚，但成为子实体在48小时。相反，overexpress几乎细胞没有流在一起，除了迁移圆柱形蛞蝓。

项目成果

Satoru Funamoto: "cAMP dependent kinase regulates Polysphondylium pallidum developmenet"Differentiation. (in press). (2002)

Satoru Funamoto：“cAMP 依赖性激酶调节苍白多毛虫发育”分化。

Nancy Van Driessche: "A transcriptional profile of multicellular development in Dictyostelium discoideum"Development. (in press). (2001)

Nancy Van Driessche：“盘基网柄菌多细胞发育的转录谱”开发。

Masatsune Tsujioka: "Spatial expression patterns of genes involved in cyclic AMP responses in Dictyostelium discoideum"Develop. Growth Differ.. 43. 275-283 (2001)

Masatsune Tsujioka：“盘基网柄菌中参与环 AMP 反应的基因的空间表达模式”开发。

落合廣: "「行動の分子生物学-山元大輔偏」分担執筆,細胞性粘菌の行動と分子"シュプリンガー・フェアラーク東京株式会社. 10 (2000)

Hiroshi Ochiai：“‘行为分子生物学 - 山本大辅’的合著者，《细胞盘基网柄菌的行为和分子》” Springer-Verlag Tokyo Co., Ltd. 10 (2000)

N.Takaoka: "Vegetatively regulated elements of the Polysphondylium pallidum gp64 promoter"Biochimica Biophysica Acat. (in press). (2002)

N.Takaoka：“苍白多毛菌 gp64 启动子的营养调控元件”Biochimica Biophysicala Acat。