Innervatiuon of steroid-producing cells in the gonads of fish.

鱼类性腺中产生类固醇的细胞的神经支配。

基本信息

批准号：
06660252
负责人：
NAKAMURA Masaru
金额：
$ 1.15万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

项目摘要

The innervation of the gonads of Oreochromis niloticus examined immunohistochemically and ultrastructurally. In testis, many thick nerve bundles are distributed in the wall surrounding the efferent duct. The neurons appear to terminate at the surface of smooth muscle cells in the efferent duct wall. Thin nerve bundles penetrate the interstices among lobules containing cysts of spermatogenic germ cells. terminal ends of these nerve fibers also make contact with smooth muscle cells enclosing lobules and with the surface of steroid-producing cells. These observations suggest that innervation of the testis may not only control the contracting and relaxing of smooth muscle cells but also control steroid secretion. In ovary, thick nerve bundles were localized in the area near the ovarian artery and vein in the ovarian wall on the side facing the mesentery. Groups of a few axons ramified from the thick nerves and were terminated in the proximity of clusters of steroid-producing cells which are distributed in the interstitial area among yolky oocytes. The axon terminals were in intimate relation with the steroid-producing cells. The terminals contained many clear vesicles, a few dense-cored vesicles and some mitochondria. Moreover, a few terminals were observed on the surface of steroid-producing theca cells surrounding yolky oocytes. Our observations of direct innervation of steroid-producing cells bring to light a possible new avenue for regulation of steroid production in the tilapia ovary.

Oreochromis Niloticus的性腺神经支配在免疫组织化学和超微结构上检查了。在睾丸中，许多厚的神经束分布在传出导管周围的壁上。神经元似乎在传出管壁中平滑肌细胞的表面终止。薄神经束穿透了含有精子生殖细胞囊肿的小叶之间的间隙。这些神经纤维的末端还与封闭小叶的平滑肌细胞以及产生类固醇的细胞表面接触。这些观察结果表明，睾丸的神经不仅可以控制平滑肌细胞的收缩和放松，还可以控制类固醇的分泌。在卵巢中，厚实的神经束位于卵巢动脉附近的区域，静脉静脉位于面向肠系膜的侧面的卵巢壁。几组轴突从厚神经中撞倒，并终止于在类固醇产生的细胞的簇中，这些细胞分布在yolky卵母细胞中的间质区域。轴突末端与产生类固醇的细胞有着密切的关系。末端包含许多透明的囊泡，一些密集的囊泡和一些线粒体。此外，在卵母细胞周围产生类固醇的蛋白质细胞的表面上观察到了一些末端。我们对产生类固醇细胞的直接支配的观察结果揭示了一种可能调节罗非鱼卵巢中类固醇产生的新途径。