Sterilization of fish by high temperature

鱼的高温灭菌

• 批准号: 23658166
• 负责人: NAKAMURA Masaru
• 金额: $ 2.41万
• 依托单位: Meio University (2013)University of the Ryukyus (2011-2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658166/
• 关键词: 魚類; 不妊化; 生殖腺; 卵巣; 精巣; 生殖細胞; ティラピア; 高水温; 体細胞; 養殖魚; コイ

Juveniles of the Mozambique tilapia, were reared from 3 days after hatching at a high temperature (37 degree centigrade) for 50 days. The gonads of all individuals were sterile. Sterile females did not display sexual behavior or exhibit female nuptial coloration, Histological analysis revealed the absence of all stages of spermatogenic germ cells from the testes of the heat-treated males; however, structures within the layer of epithelial cells lining the efferent ducts were observed to be actively secreting sperm fluid into the ducts, as also seen in the mature testes of normal males. The sterilized males nonetheless displayed male nuptial coloration, actively dug spawning nests, and mated with normal mature females. However, females mated with these males initially brooded their eggs normally but released them prematurely at 4 to 5 days. All the released eggs were unfertilized, and all were dead.

莫桑比克罗非鱼幼鱼在高温(37摄氏度)下孵化50天后，从3天开始饲养。所有个体的性腺都是不育的。不育雌性没有表现出性行为，也没有表现出雌性婚礼的颜色，组织学分析显示，热处理雄性睾丸中没有所有阶段的生精生殖细胞；然而，与正常雄性成熟睾丸一样，在传出管内的上皮细胞层内观察到活跃地将精液分泌到导管内的结构。然而，被不孕的雄性仍然表现出雄性的婚礼色彩，积极地挖掘产卵巢，并与正常成熟的雌性交配。然而，与这些雄性交配的雌性最初正常孵化它们的卵，但在4到5天后提前释放它们。所有被释放的卵子都是未受精的，而且都死了。

项目成果

Sexual behavior of sterilized male tilapia, 2013

绝育雄性罗非鱼的性行为，2013年

• 发表时间: 2013
• 作者: Selvaraj S.;et al.;Nakamura M and Nozu R
• 通讯作者: Nakamura M and Nozu R

魚類の飼育方法

怎样养鱼

Sterilization of male tilapia by high temperature

雄性罗非鱼高温灭菌

• 发表时间: 2013
• 作者: Nakamura M;他3名
• 通讯作者: 他3名

Sexual behavior of sterilized male tilapia

绝育雄性罗非鱼的性行为

• 发表时间: 2013
• 作者: Selvaraj S.;et al.;M Nakamura and R Nozu
• 通讯作者: M Nakamura and R Nozu