Studies of the expression of cell adhesion-related molecules and cytoskeletal architecture in human meningioma cells

脑膜瘤通常是良性的。然而，即使在全切除后，它们的复发率也为10- 30%，并且存在生物学上的侵袭性脑膜瘤。为了研究人脑膜瘤的细胞生物学，我们从转移性脑膜瘤衍生细胞（GMM-2）中分离出一种新的永生细胞系，该细胞系具有形成波形蛋白丝锚定桥粒（蛛网膜细胞的分化标志物）的能力。我们从GMM-2细胞中获得了30多个克隆，但大多数克隆变得衰老。我们选择了一个克隆命名为HMG-10进行进一步的研究，它达到了100的群体倍增水平。对数生长期HMG-10细胞形成上皮样细胞片层。融合后，部分细胞扩散不良，似乎是成纤维细胞样细胞（F）。13 EA 02：免疫细胞化学显示桥粒斑蛋白阳性斑点在细胞间的边界排列。细胞质点被认为是内化的桥粒。波形蛋白阳性染色质分布于细胞核周围。肌动蛋白丝主要存在于细胞周边。细胞角蛋白、上皮膜抗原和E-钙粘蛋白均为阴性。电镜下可见HMG-10细胞桥粒和大量的胞浆IF，IF锚定桥粒不仅在E细胞之间组装，而且在E细胞与F细胞之间以及F细胞之间也组装。在含有10%胎牛血清的生长培养基中，细胞呈指数生长，第一次更换培养基后倍增时间约为47小时。在20，000个细胞/平方厘米的低密度下，它们的生长受到接触抑制。我们希望通过对HMG-10细胞粘附分子表达的进一步研究，为进一步了解人脑膜瘤细胞的生长调控机制提供依据。

Meningiomas are generally benign. However, they recur with a rate of 10-30%, even after total resection, and there are biologically aggressiove meningiomas. To study the cell biology of human meningioma, we isolated a new immortal cell line with the ability to form vimentin-filament anchoring desmosome, a differentiation marker of arachnoid cell, from metastatic meningioma-derived cells (GMM-2). We obtained more than 30 clones from the GMM-2 cells, but most of the clones became senescent. We selected a clone designated HMG-10 for further study, which achived 100 population doubling level. In the log phase, HMG-10 cells formed epthelioid cell (E) sheets. After confuluence, part of them poorly spread, and appeared to bie fibroblastoid cells (F).13EA02 : Immunocytochemistry revealed that desmoplakin-positive dots were aligned at cell-to-cell boudaries. Cytoplasmic dots were considered intternalized desmosomes. Vimentin-positive filamants were distributed around the nucleus. Actin filaments were present mainly at the cell periphery. None of the cells were positive for cytokeratin, epithelial membrane antigen and E-cadherin. Electron microscopy of HMG-10 cells showed desmosomes and numerous cytoplasmic IF.IF anchoring desmosomes were assembled not only between E cells, but also between E cell and F cell as well as between F cells. In growth medium containing 10% fetal calf serum, the cells grew exponentially with a doubling time of about 47 hours after the first medium change. Their growth was contact-inhibited at a low density of 20,000 cells/cm^2. We are hopeful that further studies on the expression of cell adhesion molecules of HMG-10 cells will provide some insights into the growth control mechanism of human meningioma cells.