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Expression of cytokines and cell adhesion molecules in nasal inflammatory diseases.

鼻炎性疾病中细胞因子和细胞粘附分子的表达。

基本信息

批准号：
06671703
负责人：
OKAMOTO Yoshitaka
金额：
$ 1.41万
依托单位：
Yamanashi Medical University (1996)Akita University (1994-1995)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06671703/
关键词：
nasal mucosa cytokine cell adhesion molecule virus nasal allergy chronic sinusitis ハウスダストヒト鼻アレルギートシル酸スプラタストマウス RSウイルス G糖蛋白 F糖蛋白

项目摘要

Expression of cytokines and cell adhesion molecules was evaluated in chronic sinusitis and nasal allergy, which are the most popular nasal inflammatory diseases. Also, the effect of viral airway infection on these expressions was investigated.The results are summarized as follows.1. Employing specific reverse transcriptase polymerase chain reaction assays, mRNAs of various kinds of cytokines and cell adhesion molecules were detected in maxillary sinus mucosa of the patients with chronic sinusitis. The expression of IL-1alpha, beta was more frequently in acute phase of the disease. The mucosal T cells were thought to play an important role on the expression of these cytokines and on the pathogenesis of the disease. The extracellular matrix may participate in the persistent activation of these T cells.2. An enhanced expression of the mRNA for various cytokines was observed in nasal mucosal samples of nasal allergic subjects after the administration of allergic antigen or substance P,although the expression of IL-2 and IL-4 was low. These cytokine production may induce the cellular infiltration and the late phase reaction through the activation of cell adhesion molecules. Also, substance P may regulate allergic reaction via enhanced production of certain regulatory cytokines.3. Respiratory syncytial virus (RSV) enhanced Th-2 like cytokines production but not Th-1 like cytokines production in mitogen stimulated human tonsillar cells. In nasal epithelial cells, RSV induced the expression of intercellular adhesion molecule-1 by itself but not through production of cytokines. The enhanced synthesis of Th-2 like cytokines and cell adhesion molecules in airway infected with RSV may contribute to the unique inflammatory processes.

研究了慢性鼻窦炎和鼻变态反应中细胞因子和细胞粘附分子的表达。此外，我们还研究了呼吸道病毒感染对这些表达的影响。结果总结如下：1。采用特异性逆转录酶聚合酶链反应法检测慢性鼻窦炎患者上颌窦黏膜中各种细胞因子和细胞粘附分子的mrna。il -1 α、β的表达在疾病急性期更为频繁。粘膜T细胞被认为在这些细胞因子的表达和疾病的发病机制中起重要作用。细胞外基质可能参与这些T细胞的持续激活。在给药后，鼻黏膜中各种细胞因子的mRNA表达增强，但IL-2和IL-4的表达较低。这些细胞因子的产生可能通过激活细胞粘附分子诱导细胞浸润和后期反应。此外，P物质可能通过增强某些调节性细胞因子的产生来调节过敏反应。呼吸道合胞病毒（RSV）增强了有丝分裂原刺激的人扁桃体细胞中Th-2样细胞因子的产生，而不是Th-1样细胞因子的产生。在鼻上皮细胞中，RSV自身诱导细胞间粘附分子-1的表达，而不是通过产生细胞因子。RSV感染气道中Th-2样细胞因子和细胞粘附分子的合成增强可能是其独特的炎症过程的原因之一。