Influence of viral infection on the devefepmeat of acute otitis media and approach for treatment

病毒感染对急性中耳炎病情的影响及治疗方法

• 批准号: 12470354
• 负责人: OKAMOTO Yoshitaka
• 金额: $ 9.15万
• 依托单位: CHIBA UNIVERSITY (2002)山梨医科大学 (2000-2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470354/
• 关键词: Acute otitis media; virus; bacteria; nasal allergy; tissve culture; polymerase chain reaction; 肺炎球菌; ウイルス中和抗体; 核酸増殖法; 中耳炎動物モデル; 鼻アレルギー

Background and purpose : The role of viral infection in acute otitis media has been discussed, however, it remains obscure in detail. We investigated the influence of viral infection and of allergic sensitization in acute middle ear inflammation.Method : Microbiological examination of middle ear fluids and nasopharyngeal swabs in the children with acute otitis media was carried out. Inflammatory reaction in guinea pigs and mice which had been sensitized with ovalbumin as the primary antigen, followed by respiratory syncytial virus (RSV) inoculation we're also examined.Results : Rhinovirus in one case and RSV in 3 cases out of 34 patients were only detected in tissue-culture, but RSV in 6 cases, rhinovirus in 3 cases and adenovirus and influenza virus in each one case respectively, out of 34 cases were detected using the PCR method. The coincidence rates of the positive detection frequency of the respective virus and bacteria in the tympanic exsudate swabs in the nasopharynx was 100% in tissue-culture, 92% in the viral gene test and 50% in the bacterial examination, The children with allergic airway diseases, who had viral infection in middle ear showed highly serious medical sequelae including otitis media effusion and repeated otitis media. In animal experiment, RSV induced the excess influx of eosinophils in mucosa in those which had been sensitized with ovalbumin.Conclusion : More than 30% of the children with acute otitis media have been connected in viral infection. Allergen-sensitized mucosa showed stronger inflammatory change to viral infection than normal mucosa

背景和目的：病毒感染在急性中耳炎中的作用已有讨论，但其细节仍不清楚。探讨病毒感染和变态反应在急性中耳炎中的作用。方法：对急性中耳炎患儿的中耳液和鼻咽拭子进行微生物学检查。结果：34例患者中1例鼻病毒和3例呼吸道合胞病毒仅在组织培养中检出，6例鼻病毒、3例鼻病毒、腺病毒和流感病毒各检出1例。组织培养阳性符合率为100%，病毒基因检测阳性符合率为92%，细菌检测阳性符合率为50%。中耳有病毒感染的过敏性呼吸道疾病患儿表现出严重的医学后遗症，包括中耳炎渗出性中耳炎和反复中耳炎。在动物实验中，呼吸道合胞病毒可诱导卵蛋白致敏的儿童黏膜内嗜酸性粒细胞过度聚集。结论：30%以上的急性中耳炎患儿与病毒感染有关。变应原致敏的粘膜对病毒感染的炎性改变比正常粘膜更强

项目成果

松岡伴和, 岡本美孝: "Characteristics of immunity induced by viral antigen or conferred by antibody via different administration routes"Clinical and Experimental Immunology. 130. 386-392 (2002)

Tomokazu Matsuoka，Yoshitaka Okamoto：“病毒抗原诱导的免疫特性或通过不同给药途径抗体赋予的免疫特性”《临床和实验免疫学》130. 386-392 (2002)。

Matsuoka, T, Okamoto, Y et. Al.: "Characteristics of immunity induced by viral antigen or conferred by antibody via different administration routes"Clinical and Experimental Immunolgy. 130. 386-392 (2002)

松冈，T，冈本，Y 等。