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肺移植後の閉塞性細気管支炎の発症における気道上皮細胞の関与についての実験的研究

气道上皮细胞参与肺移植术后闭塞性细支气管炎发生发展的实验研究

基本信息

批准号：
06857086
负责人：
南正人
金额：
$ 0.58万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Encouragement of Young Scientists (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06857086/
关键词：
肺移植閉塞性細気管支炎気道上皮細胞ラット

项目摘要

現在以下の2点まですすめている.1.ラット気道上皮細胞培養系の調製について雄性inbred LEW(RT-1^1)ラット,BN(RT-1^n)ラットを麻酔下に開胸し右心よりPBSにて肺をFLUSHして気管全長・両肺プロックを摘出した.摘出ブロックの各気管支を肺門から到達できる範囲内の可及的抹消にて結紮し,得られた気管気管支樹にプロテアーゼ含有液を注入し,浸漬して4℃で一晩放置した.気管気管支内を洗浄して細胞を回収してナイロンメッシュを通したうえ遠心し培地(蛋白添加合成培地)に浮遊させた.コーティングdishに細胞浮遊を磨き37℃,5%CO2空気下に培養した.数日にてconfluentになるべく播種細胞数,培地組成,dish preparationなどの至適条件を検討中である.2.リンパ球混合培養系ならびに増殖阻止至適免疫抑制剤濃度の設定LEWラットの脾細胞を赤血球溶血除去,MMC処理のうえ刺激細胞とし,BNラットのリンパ節細胞を応答細胞としてリンパ球混合培養をおこない,免疫抑制剤としてcyclosporin AとFK-506の二剤についてBN細胞のみのコントロールリベルにまで増殖抑制を示す免疫抑制剤濃度を検討中である.

At the following two o'clock, we will start at two o'clock. The cell culture system of the epithelium of the lung was isolated from the male inbred LEW (RT- 1 ^ 1), BN (RT- 1 ^ n). The right heart was opened and the lungs were filled with PBS. The whole length of the lung tube was removed. Take out the erasure test results that can be reached in the temperature range from each branch of the tube to the lung of each branch of the tube, so that the branch of the tube is filled with liquid and placed at 4 ℃. In the branch of the tube, wash the water, wash the cells and return to the water. The cell floatation temperature is 37 ℃, and the cell floatation temperature is 37 ℃. The cell floatation temperature of dish is 37 ℃. The air temperature of 5%CO2 is low. For a few days, the number of confluent seeds sowed, the composition of the culture, and the number of dish preparation seeds in the culture conditions. 2. The mixed culture of LEW cells was blocked until the degree of immunosuppression was set. The hemolysis of red blood cells was removed in spleen cells of spleen cells, and the hemolysis of red blood cells was removed in spleen cells of spleen cells. The cells were stimulated by MMC cells, and the cells were incubated with BN cells. Immunosuppression showed that the immunosuppression was moderate in the degree of immunosuppression in the presence of cyclosporin Agar FK-506. BN cells were infected with immunosuppression.