Analysis of neuronal network for temperature regulation.

温度调节神经网络分析。

• 批准号: 07044260
• 负责人: KANOSUE Kazuyuki
• 金额: $ 4.1万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044260/
• 关键词: thermo regulation; neuronal network; set point; hypothalamus; nitric oxide

The body temperature of homeothermic animal is controlled by multiple autonomic and behabioral responses and themoreceptors are distributed throughout the body. This multiple input-output system is regulated by the central nervous system, on the top of which the hypothalamus uncluding the preoptic area is located. The neuronal network for thermoregulation has been investigated by stimulation and ablation of the brain, and the importance of the hypothalamus became apparent. In spite of the flourishing outcome of single unit studies, there is almost no advance in our knowledge of the thermoregulatory "network", which makes it difficult to link electrophysiological and pharmachological data directly with thermoregulatory responses observed in whole animals. We don't know yet, for example, what groups of neuron are working for control of each effector response, and where they send efferent signals. This project was planned to connect this "missing link" in the study of thermoregulation. Fi … More rst, we analyzed efferent projection from the proptic area for thermoregulatory vasomotion. Electrophisilogical and ablation study revealed that warm sensitive neurones of the preoptic area send (1) inhibitory signals to the ventral tegmental area and (2) excitatory signals to the PAG for thermoregulatory vasomotor bontrol. Next the results of electrical stimulations had long ago showed that the posterior hypothalamus (PH) is important for the control of shivering, but it was not clear whether it was neurons that ware stimulated or it is only passing fibers. We found that cold-induced shivering was suppressed aftor muscimol, an agonist of the GABAa receptor, was injected into the PH,which therefore must contain neurons that send excitatory signals that elicit shivering. But PO warm-sensitive neurons may send inhibitory signals directly to a lower neuronal structure, bypassing the PH,since PO warming has been found to suppress shivering in animals whose PH was isolated from other hypothalamus. Less

恒温动物的体温受多种自主神经和行为反应的控制，热感受器分布于全身。这种多输入输出系统受中枢神经系统的调节，下丘脑（包括视前区）位于中枢神经系统之上。通过刺激和消融大脑研究了体温调节的神经元网络，下丘脑的重要性变得显而易见。尽管单个单位的研究成果蓬勃发展，但我们对体温调节“网络”的认识几乎没有任何进展，这使得很难将电生理学和药理学数据与在整个动物中观察到的体温调节反应直接联系起来。例如，我们还不知道哪组神经元负责控制每种效应器反应，以及它们向何处发送传出信号。该项目计划在体温调节研究中连接这一“缺失的环节”。Fi ...更多信息 首先，我们分析了温度调节血管运动的proptic区的传出投射。电生理和消融研究表明，视前区的温敏神经元（1）向腹侧被盖区发出抑制性信号，（2）向PAG发出兴奋性信号，以调节血管温度。其次，电刺激的结果很久以前就表明，后下丘脑（PH）对控制颤抖很重要，但尚不清楚是神经元受到刺激还是只是通过纤维。我们发现，在将蝇蕈醇（一种GABA α受体激动剂）注射到PH中后，冷诱导的颤抖被抑制，因此PH中必须含有发送兴奋信号引起颤抖的神经元。但是PO温敏神经元可能会绕过PH直接向较低的神经元结构发送抑制信号，因为PO变暖已被发现抑制PH与其他下丘脑分离的动物的颤抖。少

项目成果

朝山正己: "イラスト運動生理学" 東京教学社, 145 (1995)

浅山雅美：《运动生理学图解》东京京学社，145（1995）

Pehl, U.: "Properties of temperature sensitive neurons in the rat spinal cor" J.Physiol.498. 483-495 (1997)

Pehl, U.：“大鼠脊髓温度敏感神经元的特性”J.Physiol.498。

M.Asayama: Tokyo-kyogaku-sha. Illust exercise physiology, 1-145 (1995)

M.Asayama：东京京学社。

Pierau,Fr.K.: "Specific action of GABAB ligands on the temperature sensitivity of hypothalamic neurons." Ann.N.Y.Acad.Sci.(in press).

Pierau,Fr.K.：“GABAB 配体对下丘脑神经元温度敏感性的特异性作用。”

Simon,E.: "Themosensitivity of spinal cord neurons in vivo and in vitro in comparison to hypothalamic thermosensitive neurons,Prog." Brain Res.invited review. (in press). (1997)

Simon,E.：“与下丘脑热敏神经元相比，脊髓神经元体内和体外的热敏感性，Prog。”