Optical recordings from hypothalamic neurons.

下丘脑神经元的光学记录。

• 批准号: 05557006
• 负责人: KANOSUE Kazuyuki
• 金额: $ 9.98万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05557006/
• 关键词: hypothalamus; culture; optical recording

Homeostatic functions such as body temperature regulation, eating, drinkig, and reproduction are controlled by the central nervous system including the hypothalamus. There is almost no knowledge concerning thermoregulatory "network" , which makes it difficult to link electrophysiological and pharmachological data directly with responses observed in whole animals. Present study was planed to develop a system which analyzes the network by simultaneously recording activities of many neurons from hypothalamic culture preparation. Neurons taken from the preoptic area of new-born rats (three days old) were dissociated and cultrured. Neurons survived for 7-10 days and at around day 7 long processes were observed. In ordere to make optical recordings from single cultured neurons, we tried to find out conditions for making the neurons generate action potentials. No spontaneous activity was observed not only at 37ﾟCbut also in a wide temperature range of 34-42ﾟC.Application of glutamate (1 mM-0.1 M) or K+ (10-100 mM) also failed to generate action potentials. Signal to noise ratio of the plesently-available optical recording system requires voltage change of several tens mV.So without action potentials optical recordings can not be used for analyzing neuronal networks of the hypothalamus.

体温调节、进食、饮水和生殖等稳态功能由包括下丘脑在内的中枢神经系统控制。几乎没有关于体温调节“网络”的知识，这使得很难将电生理学和药理学数据与在整个动物中观察到的反应直接联系起来。本研究拟建立一个能同时记录下丘脑培养物中多个神经元活动的神经网络分析系统。从新生大鼠（3天龄）视前区分离神经元并进行培养。神经元存活7-10天，在第7天左右观察到长过程。为了从单个培养的神经元中进行光学记录，我们试图找到使神经元产生动作电位的条件。不仅在37 ℃下，而且在34-42 ℃的宽温度范围内均未观察到自发活动。谷氨酸盐（1 mM-0.1 M）或K+（10-100 mM）的应用也未能产生动作电位。现有的光记录系统信噪比要求几十mV的电压变化，因此，没有动作电位的光记录不能用于下丘脑神经元网络的分析。

项目成果

K.Kanosue et al.: "Hypothalamic network for temperature regulation:old but still unanswered question." In:Integrative and cellulan aspect of autoncmic functions:temperature and osmoregulation. 253-257 (1994)

K.Kanosue 等人：“用于温度调节的下丘脑网络：古老但仍未解答的问题。”

K. Kanosue et al.: "Hypothalamic network for theromoregulatory vasomolor control." Am. J. Physiol.267. R283-R288 (1994)

K. Kanosue 等人：“用于体温调节血管控制的下丘脑网络”。

Shimomura, I.: "Marked reduction of Acyl-CoA synthetase (ACS) activity and mRNA,combined with reduction of lipoprotein Lipase (LPL) and glucose transporter (Glut4) m RNA,in intra-abdominal visceral fat by exercise training-Exercise may change the evnergy

Shimomura, I.：“通过运动训练，腹内内脏脂肪中酰基辅酶 A 合成酶 (ACS) 活性和 mRNA 显着降低，同时脂蛋白脂肪酶 (LPL) 和葡萄糖转运蛋白 (Glut4) m RNA 减少 - 运动可能

K, Kanosue et al.: "Hypothalamic network for thermoregulatory shivering" Am. J. Physiol.267. R275-R282 (1994)

K，Kanosue 等人：“用于体温调节颤抖的下丘脑网络”Am。

Y.-H.Zhang et al.: "Warm and cold signals from the preoptic area:which conlribute more to the control of shivering in rats?" J.Physiol.(In press).(1995)

Y.-H.Zhang 等人：“来自视前区的冷热信号：哪个对控制大鼠的颤抖更有帮助？”