Immunogenetic analysis of post-schistosomal liver disease in China

中国血吸虫后肝病的免疫遗传学分析

• 批准号: 07044290
• 负责人: HIRAYAMA Kenji
• 金额: $ 1.47万
• 依托单位: Saitama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044290/
• 关键词: Schistosomiasis japonica; China; Liver fibrosis; Ultrasound examination; HLA-DRB1; HLA-DQB1; DNA typing; PCR

Immunogenetic analysis of post-schistosomal liver fibrosis in ChinaOver 300,000 patients have been estimated to be suffering from schistosomiasis japonica in Jiangxi province along Yangsu River, its branches and Poyang lake. One of the most serious complications is post-schistosomal liver disease that develop within several years after the infection. Such chronic patients are very common so that the productivity of those areas has been depressed for many years.To identify the host genetic factors affecting the prognosis after infection, we have typed 244 of unrelated patients who are living in the endemic village named Beishan, Yushan country, Jiangxi, including 76 patients (31.1%) with no fibrotic change in the liver (Grade O) and 168 patients with typical fibrotic change including Grade I (70pts, 28.7%), Grade II (91pts, 37.3%) and Grade III (7pts, 2.9%) for their HLA-DRB1 and DQB1, by DNA typing of SSOs methods. The schistosomal fibrotic changes in the liver were observed by using ultrasound and the grades were determined by the WHO standard (Cairo1991).HLA-DRB1^<**>1101 significantly decreased in the fibrotic change group (Grade I**) indicating that this allele is resistant against fibrosis (Pcorrected<0.005, RR=0.29). On the contrary, HLA-DRB1^<**>1202 was slightly increased in the fibrosis group but it was not statistically significant. We have also typed their HLA-DQB by the same methods (n=188, grade 0=64, grade I-III=124). There observed no allele that increased or decreased in fibrotic group.Although we should examine their HLA-DQA,DPA and DPB,it is strongly suggested that the HLA-DRB1^<**>1101 is primarily associated with resistance to schistosomal liver fibrosis.

中国血吸虫病后肝纤维化的免疫遗传学分析江西省扬苏河及其支流和鄱阳湖地区约有30多万日本血吸虫病患者。最严重的并发症之一是血吸虫病后几年内发生的肝病。为了确定影响感染后预后的宿主遗传因素，我们对江西省玉山县北山村244例无血缘关系的患者进行了DNA分型，其中无肝纤维化(O级)76例(31.1%)，典型肝纤维化168例(包括I级70例，28.7%)，II级91例(37.3%)，III级7例(2.9%)。用超声观察血吸虫肝脏的纤维化改变，并按WHO(CAIRO1991)标准进行分级。纤维化改变组(I级)的HLA-DRB1；**&gt；1101显著降低，表明该等位基因对纤维化有抵抗作用(P<0.005，RR=0.29)。相反，纤维化组的HLA-DRB1^&lt；**&gt；1202略有升高，但无统计学意义。我们也用相同的方法对他们的HLADQB进行了分型(n=188，0级=，I-III级=124)。纤维化组中未发现等位基因增加或减少，虽然我们应该检测他们的HLA-DQA、DPA和DPB，但强烈建议，HLA-DRB1；**&gt；1101与血吸虫性肝纤维化的抵抗有关。

项目成果

平山謙二,陣紅根,菊池三穂子,伊藤美加子,尹東,張紹基,袁鴻昌: "中国における日本住血吸虫性肝疾患発症メカニズムの免疫遺伝学的解析" Jornal of Saitama Medical School. Vol.23. -112 (1996)

平山贤治、金洪根、菊地美穗子、伊藤美佳子、董云、张绍基、袁宏昌：“日本血吸虫肝病在中国发病机制的免疫遗传学分析”埼玉医学院学报-112。 1996）

Mikako Itho, Hori, E., Hirayama, K., Honggen Chen, Kikuchi, M., Tong Yin, Gu Xiaonan, Shoji Zhang and Hongchang Yuan: "Glycine-Valine dimorphism of 86th amino acid of HLA-DRb1 predicts theprognosis of post-schistosomal hepatic fibrosis." Jpn.Journal of Pa

Mikako Itho，Hori，E.，Hirayama，K.，Honggen Chen，Kikuchi，M.，Tong Yin，Gu Xunan，Shoji Zhu 和 Hongchang Yuan：“HLA-DRb1 第 86 个氨基酸的甘氨酸-缬氨酸二态性预测术后预后

K.Hirayama, Honggen Chen, Kikuchi, M., Tong Yin, Itoh, M.Gu Xiaonan, Shoji Zhang and Hongchang Yuan.: "A strong association between HLA-DRB1^<**>1101 and resistance to post-schistosomal liver fibrosis in China." International Congress of Immunology.9.145

K.Hirayama, Honggen Chen, Kikuchi, M., Tong Yin, Itoh, M.Gu Xunan, Shoji Zhang 和 Hongchang Yuan.：“HLA-DRB1^<**>1101 与血吸虫后肝脏抵抗力之间存在很强的相关性

平山謙二,陣紅根,菊池三穂子,伊藤美加子,尹東,張紹基,袁鴻昌: "Glycine/Valine dimorphism of 86th amino acid of HLA-DRB1 predicts the prognosis of post schistosomal liver disease." The 30th Japan-United states Joint Cooperative medical Science Program.Vol.30. -38 (1995)

Kenji Hirayama、Bengen Jin、Mihoko Kikuchi、Mikako Ito、Dong Yun、Shaoji Zhang、Hongchang Yuan：“HLA-DRB1第86位氨基酸的甘氨酸/缬氨酸二态性预测血吸虫后肝病的预后”第30届日本-美国。联合合作医学科学计划。第30卷-38（1995）

平山謙二,陣紅根,菊池三穂子,伊藤美加子,尹東,張紹基,袁鴻昌: "A strong association between HLA-DRB1^★1101 and resistance to post-schistosomal liver fibrosis in China." The 9th International Congress of Immunology.Vol.9. -145 (1995)

Kenji Hirayama、Bengen Jin、Mihoko Kikuchi、Mikako Ito、Dong Yun、Shaoji Zhang、Hongchang Yuan：“HLA-DRB1^★1101 与中国血吸虫后肝纤维化抵抗力之间的密切相关性第九届国际免疫学大会”。 .卷 9.-145 (1995)