Immunogenetic analysis of schistosomiasis japonica in China

我国日本血吸虫病免疫遗传学分析

• 批准号: 10044317
• 负责人: HIRAYAMA Kenji
• 金额: $ 3.2万
• 依托单位: Saitama Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044317/
• 关键词: Schistosoma japonicum; re-infection study; HLA; cDNA library; China; Protective immunity; mass-treatment; liver fibrosis; 日本住血吸虫症; 高リスク集団; 遺伝

1)Re-infection study was re-challenged in the new target village, Caipeng, in Jiangxi.180 persons (29.2% of the total examined) in the village were fecal egg positive in December 1998. All the patients were treated in March, 1999 and re-examined for their re-infection in December, 1999. Almost 60 persons were re-infected. Those susceptible persons will be analyzed for their HLA types. We have already found in Zhuxi that there was a special resistant HLA-DR type present. We will see if this is reproducible the new village.2)Immunogenetic analysis of the post-schistosomal liver fibrosis.To identify the responsible antigen that will stimulate or modulate the fibrotic repose to eggs, we screened recombinant antigens from an expression library of eggs using positive human serum. 200 positive clones were isolated and 146 clones out of those were directly sequenced. The result was unexpected because all the clones were several parts of one gene named miracidial antigen. The deduced amino acid sequence had no binding motif of resistant HLA-DRB1*11O1 but had a motif of susceptible HLA-DRB1* 1501. The lymphocyte study should be done to see their real reactivity to this recombinant antigen.

1）在江西省新设的目标村蔡棚村进行再感染研究，1998年12月该村粪虫卵阳性180人，占受检总人数的29.2%。所有患者均于1999年3月接受治疗，并于1999年12月重新检查是否再次感染。近60人再次感染。这些易感者将被分析其HLA类型。我们在竹溪发现了一种特殊的耐药HLA-DR型。我们将看看这是否是可重复的新的村庄。2）后染色体肝纤维化的免疫遗传学分析。为了鉴定将刺激或调节纤维化对鸡蛋的依赖的负责抗原，我们使用阳性人血清从鸡蛋的表达文库中筛选重组抗原。共分离到200个阳性克隆，其中146个克隆进行了直接测序。结果出乎意料，因为所有克隆都是一个名为毛蚴抗原的基因的几个部分。推导的氨基酸序列不含耐药HLA-DRB 1 * 11 O 1结合基序，但含有易感HLA-DRB 1 * 1501结合基序。应进行淋巴细胞研究以观察其对该重组抗原的真实的反应性。

项目成果

Kenji Hirayama et.al.: "HLA-DR-DQ alleles and HLA-DP alleles are independently associated with susceptibllity to different stages of post-schlstosomal hepatic fibrosis in the Chinese population." Tissue antigens. 53. 269-274 (1999)

Kenji Hirayama 等人：“HLA-DR-DQ 等位基因和 HLA-DP 等位基因与中国人群对不同阶段的血吸虫后肝纤维化的易感性独立相关。”

平山謙二: "寄生虫感染の重症化に関する遺伝子"医学のあゆみ. 191(1). 124-130 (1999)

Kenji Hirayama：“与寄生虫感染严重程度相关的基因”，《医学史》191(1)。

Hongg Chen.: "Vaccination of domestic pig with recombinant paramyosin against Schistosoma Japonicum in China."Vaccine. (in press).

Hongg Chen.：“中国用重组副肌球蛋白对家猪进行抗日本血吸虫疫苗接种。”疫苗。

Honggen Chen: "Vaccination of domestic pig with recombinant paramyosin against Schistosoma Japonicum in China"Vaccine. (in press). (2000)

陈宏根：“我国用重组副肌球蛋白接种家猪抗日本血吸虫”疫苗。