Immunogenetic analysis of schistosomiasis japonica in China

1)江西彩蓬村再次开展再感染调查。1998年12月，该村180人(29.2%)粪卵阳性。所有患者均于1999年3月接受治疗，并于1999年12月再次检查是否再次感染。近60人再次感染。这些易感人群将被分析他们的人类白细胞抗原类型。我们已经在竹溪发现存在一种特殊的耐药的人类白细胞抗原-DR类型。2)血吸虫病后肝纤维化的免疫遗传学分析。为了确定刺激或调节虫卵纤维化休眠的负责抗原，我们使用阳性人血清从虫卵表达文库中筛选重组抗原。从中分离出200个阳性克隆，对其中146个克隆进行直接测序。结果出乎意料，因为所有的克隆都是一种名为毛虫抗原的基因的几个部分。推导出的氨基酸序列中没有抗性的HLADRB1*1101结合基序，而有敏感的HLADRB1*1501基序。应该进行淋巴细胞研究，以了解它们对这种重组抗原的真正反应性。

1)Re-infection study was re-challenged in the new target village, Caipeng, in Jiangxi.180 persons (29.2% of the total examined) in the village were fecal egg positive in December 1998. All the patients were treated in March, 1999 and re-examined for their re-infection in December, 1999. Almost 60 persons were re-infected. Those susceptible persons will be analyzed for their HLA types. We have already found in Zhuxi that there was a special resistant HLA-DR type present. We will see if this is reproducible the new village.2)Immunogenetic analysis of the post-schistosomal liver fibrosis.To identify the responsible antigen that will stimulate or modulate the fibrotic repose to eggs, we screened recombinant antigens from an expression library of eggs using positive human serum. 200 positive clones were isolated and 146 clones out of those were directly sequenced. The result was unexpected because all the clones were several parts of one gene named miracidial antigen. The deduced amino acid sequence had no binding motif of resistant HLA-DRB1*11O1 but had a motif of susceptible HLA-DRB1* 1501. The lymphocyte study should be done to see their real reactivity to this recombinant antigen.