Immunogenetic analysis of schistosomiasis japonica in China

1)在江西省彩棚村重新开展再感染研究，1998年12月该村粪卵阳性180人（占总检人数的29.2%）。所有患者于1999年3月接受治疗，12月复查是否再次感染。近60人再次受到感染。对易感人群进行HLA型分析。我们已经在朱溪发现了一种特殊的耐药HLA-DR型。我们将看看这是否能在新村重现。2)血吸虫后肝纤维化的免疫遗传学分析。为了确定刺激或调节卵细胞纤维化反应的抗原，我们使用阳性人血清从卵细胞表达文库中筛选重组抗原。共分离到200个阳性克隆，其中146个克隆直接测序。结果出乎意料，因为所有克隆都是一个名为miracidial antigen的基因的几个部分。推导出的氨基酸序列不存在抗性HLA-DRB1*11O1的结合基序，但存在易感HLA-DRB1* 1501的结合基序。应进行淋巴细胞研究，以了解它们对重组抗原的真正反应性。

1)Re-infection study was re-challenged in the new target village, Caipeng, in Jiangxi.180 persons (29.2% of the total examined) in the village were fecal egg positive in December 1998. All the patients were treated in March, 1999 and re-examined for their re-infection in December, 1999. Almost 60 persons were re-infected. Those susceptible persons will be analyzed for their HLA types. We have already found in Zhuxi that there was a special resistant HLA-DR type present. We will see if this is reproducible the new village.2)Immunogenetic analysis of the post-schistosomal liver fibrosis.To identify the responsible antigen that will stimulate or modulate the fibrotic repose to eggs, we screened recombinant antigens from an expression library of eggs using positive human serum. 200 positive clones were isolated and 146 clones out of those were directly sequenced. The result was unexpected because all the clones were several parts of one gene named miracidial antigen. The deduced amino acid sequence had no binding motif of resistant HLA-DRB1*11O1 but had a motif of susceptible HLA-DRB1* 1501. The lymphocyte study should be done to see their real reactivity to this recombinant antigen.