IMMNOGENETIC ANALYSIS OF SCHISTOSOMIASIS JAPONICA IN CHINA

我国日本血吸虫病免疫遗传学分析

• 批准号: 08044316
• 负责人: HIRAYAMA Kenji
• 金额: $ 3.07万
• 依托单位: SAITAMA MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044316/
• 关键词: Schistosoma japonicum; Chemotherapy; Re-infection; China; HLA; Susceptibility; Resistance; Immunity; 日本住血吸虫; 集団治療; HLA-DRB1; DNAタイピング

Along Yangzhe River in China, Schistosomiasis japonica is still endemic. we have been estimating the efficiency of the mass-therapy on the prevalence of several targeted villages in Jiangxi. We observed that there were two categories, resistant and susceptible, in the levels of re-infection. Our final goal of this study is to clarigy the host factors which determine their susceptiblity.Method. Zhuxi village is located on the northern shore of Poyang Lake in Xingzi County, Jiangxi Province. Transmission occurs mainly during the flood season which usually begins in mid-May and goes through October. The life of the community is based on substance agriculture. Fishing is also important in this area. The reinfection study began from March to December 1995. In the beginning of study, before mass chemotherapy, all villagers at home were asked to give a stool specimen and duplicate 41.7mg Kato thick smears were examined quantitatively for S.japonicum eggs. Then, in Dec.1995, reinfection rate a … More nd intensity were examined for those subjects. Meanwhile, venous blood samples were collected twice at the same time as stool examinations. Nanshan is an islet endemic village, which located in the center of Poyang Lake, of Xinjian county, Jiangxi Province. The infection rate of Nanshan from 1991 to 1994 were 30.4%, 33.4%, 20.7%, 17.2% resoectively. Agriculture and fishing are the main income of this village. In February 1997, a retrospective study was designed to select subjects who are relative susceptible or resistant to reinfection based on a five-year Kato-Katz records (1990-1994).Results We have categorized two groups in the subjects in Zhuxi and Nanshan by using egg count record. For Zhuxi, the resistant should have less than 100 eggs in both exams. For Nanshan, the resistant should not have any positive egg count during 5 years period. Then we have typed them for their HLA-DRB1 alleles as shown in Table 1. Compared with the resistant individuals, normal people had higher frequency of HLA-DRB1^<**>0301 (Odds Ratio=4.13,95% Confidence Interval =1.31-13.0) and HLA-DRB1^<**>1201 (OR=3.80,95%CI=1.02-d14.2). Therefor, those two allels are associated with susceptibility to re-infection. Those associations are not significant after the Bonferroni correction, so it would be necesary to increase the number of the subjects to confirm the association. We are planning to expand the field and to analyze other polymorphic genetic loci that relate the immune responsiveness. Less

日本血吸虫在我国沿着长江流域仍有流行。我们一直在评估江西省几个目标村的群众治疗的效果。我们观察到，有两个类别，耐药和敏感，在再感染的水平。本研究的最终目的是阐明决定其易感性的宿主因素。竹溪村位于江西省星子县鄱阳湖北方海岸。传播主要发生在通常从5月中旬开始到10月结束的汛期。社区的生活以物质农业为基础。渔业在这方面也很重要。再感染研究从1995年3月至12月开始。研究开始时，在集体化疗前，所有村民都被要求在家中提供粪便标本，并重复进行41.7mg加藤厚涂片定量检查日本血吸虫卵。然后，在1995年12月， ...更多信息 并对这些受试者进行了强度检查。同时，在粪便检查的同时采集两次静脉血样本。南山是江西省新建县鄱阳湖中心的一个海岛型地方村落。南山区1991 ~ 1994年感染率分别为30.4%、33.4%、20.7%、17.2%。农业和渔业是这个村庄的主要收入。1997年2月，根据Kato-Katz法1990-1994年5年的病例资料，选择对再感染相对敏感或抵抗的人群进行回顾性研究。对于竹溪，抵抗者在两次测试中的卵数应低于100个。南山鸡在5年内不应出现阳性虫卵。然后我们对他们的HLA-DRB 1等位基因进行分型，如表1所示。与耐药个体相比，正常人HLA-DRB 1 ^**>0301（OR = 4.13，95%CI =1.31-13.0）和HLA-DRB 1 ^**>1201（OR= 3.80，95%CI =1.02-d14.2）的频率较高。因此，这两个等位基因与再感染的易感性相关。这些关联在Bonferroni校正后不显著，因此增加受试者的数量以确认关联是必要的。我们正计划扩大这一领域，并分析与免疫反应相关的其他多态性遗传位点。少

项目成果

Nobuo Ohta, Yukio Hosaka, Yasuo Nakajima, Masanori Sho, Tamotsu Kanazawa, Makoto Itoh, Kenji HIrayama, Masaru Minai, Daren Zhou, Yen Chen: "Endemic status of schistosomiasis japonica in Human Province, Peoples Republic of China. Preliminary study in a vil

Nobuo Ohta、Yukio Hosaka、Yasuo Nakajima、Masanori Sho、Tamotsu Kanazawa、Makoto Itoh、Kenji HIrayyama、Masaru Minai、Daren Zhou、Yen Chen：“中华民国人类省日本血吸虫病的流行状况。在一个村庄的初步研究

Kenji Hirayama: "Schistosomiasis japonica" Today's Therapy 1997 (Igakushoin.). 187. (1997)

Kenji Hirayyama：“日本血吸虫病”今日治疗 1997 年（医学书院）。

Kenji HIrayama, Hongeen Chen, Mihoko Kikuchi, Tong Yin, Mikako Itoh, XiaonanGu, Shaoji Zhang, and Hongchang Yuan.: "Glycine-Valine dimorphism at the 86th amino acid of HLA-DRB1 influenced the prognosis of post-schistosomal hepatic fibrosis." The Jounal of

Kenji Hirayama、Hongeen Chen、Mihoko Kikuchi、Tong Yin、Mikako Itoh、XiaonanGu、Shaoji Zhang 和 Hongchang Yuan.：“HLA-DRB1 第 86 个氨基酸的甘氨酸-缬氨酸二态性影响了血吸虫后肝纤维化的预后。”

Kenji Hirayama: "Glycine-Valine Dimorphism at the 86th Amino Acid of HLA-DRB1 Influenced the Prognosis of Postschistosomal Hepatic Fibrosis" Journal of Infectious Diseases. 13. 17-29 (1998)

Kenji Hirayyama：“HLA-DRB1 第 86 个氨基酸的甘氨酸-缬氨酸二态性影响了血吸虫后肝纤维化的预后”《传染病杂志》。

太田 伸生: "中国湖南省の日本住血吸虫病調査;楊子江流域の1村落をモデルとして" 熱帯. 30. 93-99 (1997)

Nobuo Ota：“中国湖南省日本血吸虫病调查；以长江流域的一个村庄为模型”热带。 30. 93-99 (1997)