Animal model for the treatment of Chronic Chagas Disease using the combination of drug and vaccine

为了寻找治疗慢性查加斯成人患者的新策略，我们尝试用小鼠和荧光素酶基因转基因的克氏锥虫Y株建立慢性感染模型，并成功地获得了利用全身成像系统观察慢性状态的方案。然后，我们利用已报道的TcG1、TcG2和TcG4三个候选基因和我们最近建立的pVAX 200DEST纳米佐剂制备了DNA疫苗，观察其对清除慢性感染寄生虫的效果。疫苗的评估尚未完成，但疫苗接种可能会通过B细胞和T细胞水平产生实质性的免疫效果。

To find a new strategy to treat a chronic Chagas adult patient, we tried to establish a chronic infection model with mouse and luciferase gene transfected Y strain of Trypanosoma cruzi and successfully obtain a protocol to observe the chronic status using whole body imaging system. Then we prepared the DNA vaccine using previously reported three candidate genes namely TcG1, TcG2 and Tcg4 and our recently established pVAX 200DEST nano-particle adjuvant to observe that effect on the elimination of chronically infected parasites. The evaluation of the vaccines has not yet finished but the vaccination could provoke substantial immunological effect by B and T cell levels.