Genetic susceprtibity to acute or chronic schistosomiasis japonica

因为T细胞反应在发病机制中起着最重要的作用，所以我们寻找能够刺激这些单倍型阳性个体T细胞的主要抗原。用lambda gt11 I噬菌体系统从中国株新鲜分离的虫卵中提取mRNA，构建了文库，并用免疫球蛋白G检测系统筛选与混合血清反应的主要抗原。最后分离出200个阳性克隆，对其中146个克隆进行直接测序。结果出乎意料，因为所有的克隆都是一种名为毛虫抗原的基因的几个部分。结论：1.克隆的基因在GST融合蛋白系统中成功表达。T…再感染的研究更多三个不同的村庄。我们发现了两件事。一是在人群中没有年龄依赖性的抵抗力，二是存在两类人，对再次感染具有抵抗力和易感性。我们发现耐药者共用其第67个氨基酸苯丙氨酸，而不是67个亮氨酸或异亮氨酸。由于67F或I/L对于形成结合口袋很重要，因此激发一些保护性免疫反应将是至关重要的。在中国疫区，控制猪和水牛等宿主宿主的感染是非常重要的。我们已经用家猪和重组副肌球蛋白进行了疫苗试验研究。对80多头猪的广泛研究表明，这些猪对攻击感染很敏感，在接种副肌球蛋白和减毒尾蚴(疫苗正在出版中)后，它们对感染具有免疫力。较少

1.Molecular cloning of the major antigens from a cDNA library of schistosomal egg by using patients pooled sera.We have already found there are relatively resistant and susceptible HLA-class II haplotypes present in the Chinese patients against post-schistosomal liver fibrosis. This 'ear we seeked for the major antigens that would stimulate those HLA haplotypes positive individuals' T cells, because T cell response is assumed to be the most important in the pathogenesis. A cDNA library was prepared from a mRNA extracted from the freshly isolated eggs of a Chinese strain using lambda gt11 I phage system and was screened for the major antigens reactive with pooled sera by IgG detection system. Finally 200 positive clones were isolated and 146 clones out of those were directly sequenced. The result was unexpected because all the clones were several parts of one gene named miracidial antigen. The cloned gene was successfully expressed by GST fusion protein system.2. Re-infection study in t … More hree different villages.We have found two things. One is that there was no age dependent resistance in the population and the other is that there were two categories of people present, resistant and susceptible against re-infection. We have found that the HLA-DRB1 types that resistant persons had shared its 67th amino acid, phenylalanine, instead of having 67 leucine or isoleucine. Because the 67F or I/L is important to make a binding pocket, it would be critical to provoke some protective immune response.3. Vaccination trial of domestic pigs with recombinant paramyosin in China.In the endemic area in China, it is important to control the infection of the reservoir hosts, such as pigs and water buffaloes. We have performed a vaccine trial study using domestic pigs and recombinant paramyosin. The extensive study using more than 80 pigs revealed that the pigs were susceptible to challenge infection and were provoked immunity against infection after the vaccination with paramyosin as well as with attenuated cercariae (Vaccine in Press). Less