Immunogenetic analysis of pathogenesis of Chagas Disease in South America.

1. 玻利维亚克氏锥虫致病性的遗传分析目的：鉴定引起慢性恰加斯病心脏病和巨结肠的克氏锥虫致病性菌株。结果：在玻利维亚圣克鲁斯的三个诊所和医院收集了三个不同临床组的血液样本，不确定、心脏和巨结肠。从这些样本中提取10m1外周血制备DNA。约60%血清阳性患者使用基因组核糖体RNA基因和动质体微环基因PCR反应阳性。所鉴定的寄生虫基因均为已报道的谱系IId。利用微环dna多态性，在我们的样本中鉴定出更多的亚谱系。但亚系与致病性之间没有相关性。讨论：虽然成功地扩增了外周血样本中的DNA片段，但没有典型的亚谱系特异性地引起恰加斯或巨结肠恰加斯病。两者的致病差异有待进一步研究，但宿主遗传因素很可能对恰加斯病的临床预后影响更大。决定玻利维亚慢性恰加斯病临床形式的宿主遗传因素的免疫遗传学分析。目的：探讨预测慢性恰加斯病患者临床预后的宿主遗传因素。结果：收集了60例不确定型患者、71例心脏恰加斯型患者、72例巨结肠患者的血样，提取了DNA。从这些样本中测定HLA-DRB1等位基因，并比较各组间每个等位基因的频率。到目前为止，没有任何DRB1等位基因与临床类型之间的显著关联。讨论：先前在危地马拉的研究表明心脏恰加斯病与属于I类基因的HLA-B35和MICA5之间存在关联。在这两项使用HLA-DRB1的研究中，负相关是可重复的。我们现在正在分析hla I类基因，HLA-A， B和MICA。如果在I类基因等位基因和心脏病之间存在任何可重复的关联，这将是有趣的。少

1. Genetic analysis of pathogenisity of Trypanosoma cruzi in BoliviaPurpose : To identify any pathogenic strain of T.cruzi causing cardiopathy and or megacolon in the chronic Chagas Disease.Results : Collection of the blood samples from three different clinical groups, indeterminate, cardiac, and megacolon at three clinics and hospitals in Santa Cruz, Bolivia. DNA extracted from 10m1 peripheral blood was prepared from those samples. About 60% of the seropositive patients showed positive PCR reactions using genomic ribosomal RNA genes and Kinetoplast minicircle genes. All the identified parasite genes were clearly shown to be the lineage IId that had already reported. Using Minicircle DN A polymorphism, more sublineages were identified within our samples. But there was no co-relation between the sublineages and their pathogenicity.Discussion : Although it was successful to amplify the DNA fragments in the peripheral blood samples, there was no typical sublineages specifically provoke ca … More rdiac Chagas or megacolon Chagas. Further study will be needed to analyse their pathogenic differences, however, it is highly possible that host genetic factors are more influential to predict clinical prognosis of Chagas disease.2. Immunogenetic analysis of host genetic factors determining their clinical forms of chronic Chagas in Bolivia.Purpose : To identify host genetic factors that can predict the patients clinical prognosis in chronic Chagas.Results : We have collected 60 patients with Indeterminate, 71 pts with cardiac Chagas, and 72 patients with megacolon and got DNA from the blood samples. HLA-DRB1 alleles were determined from those samples and the frequencies of each allele were compε red between the groups. So far, there was no significant association between any DRB1 alleles and the clinical types.Discussion : Previous study in Guatemala showed an association between cardiac Chagas and HLA-B35, and MICA5 that are belonging to class I genes. The negative association was reproducible in those two studies using HLA-DRB1. We are now analyzing HLA-class I genes, HLA-A, B and MICA. It will be interesting if there are any reproducible association between class I gene alleles and cardiac. Less