Molecular Biological Studies for the Evolution of the Nervous System as an Informational Processing System

神经系统作为信息处理系统进化的分子生物学研究

• 批准号: 07409009
• 负责人: YAMAMORI Tetsuo
• 金额: $ 12.48万
• 依托单位: OKAZAKI NATIONAL RESEARCH INSTITUTE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07409009/
• 关键词: nervous system; neocortex; memory; cytokines; brain evoution; gene expression; 長期記憶; 小脳LTD; Jun-B; AMPA; AMAP

1. Long-term memory and gene expressionWe have examined gene expression of cerebellar Purkinje cells under the condition that they received the conjunctive stimuli of parallel fibers and climbing・ fibers. We found that immediate early genes, particularly jun-B, were induced when pharmacologically mimicked conjunctive stimuli were applied into Purkinje cells in slices and in vivo animals.2. Gene expression under audio-visual discrimination tasksSakata and Kitsukawa in collaboration with Dr. Yoshio Sakurai (Kyoto University, the faculty of Literature) examined the gene expression of c-fos when rats were proceeding to an audio-visual descimination task and found the expression was enhanced in the auditory cortex when rats were working for audio tasks.3. An novel type RNA: rat Brain Specific Repetitive (Bsr) RNAKomine found a novel type of RNA, rat brain specific repetitive (Bsr) RNA. This RNA is unique in that it is repetitive RNA specifically expressed in the rat brain and only exists in … More the rattus.4. Finding dichromatic monkeysColor deficiency, mostly linked to X-chromosome, is found about several percent in human population. However, no color-defective monkeys were reported in old world monkeys whose genes including color pigment genes are very similar to humans. By analyzing DNA samples of 3 153 macaques, Onishi and Koike found three dichromatic monkeys. The frequency of dichromats in monkeys is significantly lower than that of humans.5. Genes specifically expressed in specific areas of the monkey neocortexThe neocortex is particularly evolved in the mammal and plays the major role in higher functions of the brain. It can be separated into distinct functional areas. By now, it has not been well understood how these areas are genetically programmed or determined by environmental inputs. We are trying to approach these questions by identifying the genes that are specifically expressed in the specific areas of neocortex. Tochitani found a gene named OCC-1 specifically expressed in the occipital cortex and Watakabe found the expression of GDF7 is higher in the mortor cortex than other are as. Less

1. 长期记忆和基因表达我们检测了小脑浦肯野细胞在平行纤维和攀爬纤维联合刺激下的基因表达。我们发现，当药理学模拟的联合刺激应用于切片和活体动物的浦肯野细胞时，直接早期基因，特别是jun-B，被诱导。akata和Kitsukawa与Yoshio Sakurai博士（京都大学文学系）合作，研究了大鼠在进行视听消除任务时c-fos的基因表达，发现当大鼠在进行音频任务时，听觉皮层中的表达增强。一种新型RNA：大鼠脑特异性重复RNA (Bsr) RNAKomine发现了一种新型RNA，大鼠脑特异性重复RNA （Bsr）。这种RNA的独特之处在于它是在大鼠大脑中特异性表达的重复RNA，并且只存在于……发现二色猴子颜色缺乏，主要与x染色体有关，在人类中发现了大约百分之几。然而，在包括色素基因在内的基因与人类非常相似的旧大陆猴子中，没有发现有颜色缺陷的猴子。通过分析3153只猕猴的DNA样本，大西和小池发现了三只二色猴子。猴子出现二色视的频率明显低于人类。猴新皮质在哺乳动物中特别进化，在大脑的高级功能中起主要作用。它可以被分成不同的功能区。到目前为止，人们还没有很好地理解这些区域是如何被遗传编程或由环境输入决定的。我们正试图通过识别在新皮层的特定区域特异性表达的基因来解决这些问题。Tochitani发现一种名为OCC-1的基因在枕叶皮层特异性表达，Watakabe发现GDF7在运动皮层的表达高于其他as。少

项目成果

山森哲雄: "コリン作動性因子CDF/白血病抑制因子LIFの免疫系と神経系における進化" 自律神経. 32巻3号. 286-290 (1995)

Tetsuo Yamamori：“免疫系统和神经系统中胆碱能因子 CDF/白血病抑制因子 LIF 的进化”，自主神经学，第 32 卷，第 3 期，286-290（1995 年）。

Fujiwara, T. 等: "Interaction of HPC-1/syntaxin 1A with the cytoskeletal protein, tubulin"Biochem. Biophy. Res. Comm.. 231. 352-355 (1997)

Fujiwara，T.等人：“HPC-1/突触蛋白1A与细胞骨架蛋白微管蛋白的相互作用”Biochem.Res.231.352-355(1997)

Onishi , A., Koike, S., Ida, M., Imai, H., Shichida, Y., Takenaka, O., Hanzawa, A., Komatzu, H., Mikami, A., Goto, S., Suryobroto, B., Kitahara, K. And Yamamori, T.: "Dichiromatism in macaque monkeys"Nature. 422. 140-141 (1999)

大西，A.，小池​​，S.，井田，M.，今井，H.，七田，Y.，竹中，O.，半泽，A.，小松，H.，三上，A.，后藤，S.，

Fujiwara, T,. Yamamori, T., Yamaguchi, K., and Akagawa, K: "Interaaction of HPC-1/syntaxin 1A with the cytoskeletal protein, tubulin"Biochem. Bilphy. Res. Communi. 231. 352-355 (1997)

藤原，T.

Yano, R. 等: "Cerebellar long-term plasticity and gene expression"Proceding of the Uehara Memorial Foundation Symposium on integrative and molecular approach to brain function, Tokyo, 25-27 June 1996 (Ed., Miyashita, Y. and Ito, M.). 35-44 (1996)

Yano, R. 等人：“小脑长期可塑性和基因表达”上原纪念基金会脑功能综合和分子方法研讨会论文集，东京，1996 年 6 月 25-27 日（编辑，Miyashita, Y. 和伊藤，M.）35-44（1996）。