functional studies of genes selectively expressed in primate eocortical areas

灵长类皮质区选择性表达基因的功能研究

• 批准号: 20240030
• 负责人: YAMAMORI Tetsuo
• 金额: $ 29.87万
• 依托单位: National Institute for Basic Biology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20240030/
• 关键词: 大脳皮質; 領野; 視覚野; 連合野; 霊長類; ウイルスベクター; 遺伝子発現; 大脳皮質領野; AAV; PET; 視聴覚弁別課題; 連合刺激; 反応速度; 2次視覚野; 上丘; 領域; 視聴覚統合; V2L

First, we aimed to analyze the function of the genes selectively expressed in the primate neocortical areas in comparison with that in rodents. We examined the expression of c-Fos when rats were subjected to an audio-visual multisensory task. We found that specific enhancement of c-Fos expression in V2L (the lateral secondary visual area) upon being exposed to the task. Using previously developed cortical box method, we analyzed three layer specific genes (RORb, ER81, Nurr1) in the rat neocortex. Each of three genes showed a mutually exclusive and complimentary expression pattern, which demonstrated that the areas in rodents were able to be automatically or objectively described although the area selective expression was not as prominent as that in primates. We reported SLIT1 as a gene that was selectively expressed in the association areas of the macaque neocortex. Finally, we have established the gene manipulation system in the primate neocortex

首先，我们的目的是分析选择性表达的基因在灵长类动物的新皮层区的功能与啮齿类动物的比较。我们研究了c-Fos的表达时，大鼠进行视听多感觉任务。我们发现，特定的增强c-Fos表达在V2 L（外侧第二视觉区）后，暴露于任务。我们利用先前开发的皮质盒方法，分析了大鼠新皮质中的三个层特异性基因（RORb，ER 81，Nurr 1）。这三个基因的表达模式都是互斥互补的，说明啮齿类动物的脑区是能够被自动或客观描述的，尽管其脑区选择性表达不如灵长类动物显著。我们报告SLIT 1作为一个基因，选择性地表达在猕猴新皮层的联合区。最后，我们在灵长类动物大脑新皮层中建立了基因操作系统

项目成果

Prefrontal-enriched SLIT1 expression in Old World monkey cortex established during the postnatal development.

• DOI: 10.1093/cercor/bhp319
• 发表时间: 2010-10
• 期刊: Cerebral cortex (New York, N.Y. : 1991)
• 作者: Sasaki T;Komatsu Y;Watakabe A;Sawada K;Yamamori T
• 通讯作者: Yamamori T

The ocular dominance column and gene expression upon light stimulation in the marmoset primary visual cortex

狨猴初级视觉皮层光刺激下的眼优势柱和基因表达

• 发表时间: 2013
• 作者: 磯山直也;寺田努;塚本昌彦;横関明男;山森哲雄
• 通讯作者: 山森哲雄

マーモセット一次視覚野において光刺激で惹起される遺伝子群の経時的発現解析

狨猴初级视觉皮层光刺激诱导基因的时程表达分析

• 发表时间: 2012
• 作者: Fujisawa;Hironori;K.Gohara;仲神友貴
• 通讯作者: 仲神友貴

Gene expression patterns in visual cortex during the critical period: Synaptic stabilization and reversal by visual deprivation

• DOI: 10.1073/pnas.0710172105
• 发表时间: 2008-07-08
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Lyckman, Alvin W.;Horng, Sam;Sur, Mriganka
• 通讯作者: Sur, Mriganka

Paraneoplastic antigen-like 5 gene (PNMA5) is preferentially expressed in the association areas in a primate specific manner.

• DOI: 10.1093/cercor/bhp062
• 发表时间: 2009-12
• 期刊: Cerebral cortex (New York, N.Y. : 1991)
• 作者: Takaji M;Komatsu Y;Watakabe A;Hashikawa T;Yamamori T
• 通讯作者: Yamamori T