functional studies of genes selectively expressed in primate eocortical areas

首先，我们旨在分析灵长类动物新皮质区选择性表达的基因的功能，并将其与啮齿类动物进行比较。我们检测了大鼠在接受视听多感觉任务时c-Fos的表达。我们发现，在暴露于任务后，V2L（外侧第二视觉区）的c-Fos表达特异性增强。采用先前开发的皮质盒法，我们分析了大鼠新皮层中的三个层特异性基因（RORb, ER81, Nurr1）。三个基因均表现出互斥互补的表达模式，表明啮齿类动物的区域选择性表达不像灵长类动物那样突出，但具有自动或客观描述区域的能力。我们报道了SLIT1是一个在猕猴新皮层的关联区选择性表达的基因。最后，我们建立了灵长类动物新皮层的基因操作系统

First, we aimed to analyze the function of the genes selectively expressed in the primate neocortical areas in comparison with that in rodents. We examined the expression of c-Fos when rats were subjected to an audio-visual multisensory task. We found that specific enhancement of c-Fos expression in V2L (the lateral secondary visual area) upon being exposed to the task. Using previously developed cortical box method, we analyzed three layer specific genes (RORb, ER81, Nurr1) in the rat neocortex. Each of three genes showed a mutually exclusive and complimentary expression pattern, which demonstrated that the areas in rodents were able to be automatically or objectively described although the area selective expression was not as prominent as that in primates. We reported SLIT1 as a gene that was selectively expressed in the association areas of the macaque neocortex. Finally, we have established the gene manipulation system in the primate neocortex