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Phylogenetic analyzes of the family Ranidae from DNA sequences

根据 DNA 序列对蛙科进行系统发育分析

基本信息

批准号：
07454234
负责人：
MATSUI Masafumi
金额：
$ 2.56万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07454234/
关键词：
Amphibia Systematics mitochondrial DNA cytochrome b Ranidae Rana

项目摘要

This study was made as a part of phylogenetic analyzes of the family Ranidae, which nobody has succeeded since 1920, by applying a new method of comparing DNA sequences to clarify relationships among related taxa.First, phylogenetic relationships among all known 10 nominate taxa of Japanese brown frogs (Rana pirica, R.dybowskii, R.ornativentris, R.japonica, R.tagoi tagoi, R.t.yakushimensis, R.t.okiensis, R.sakuraii, R.tsushimensis, and R.okinavana) are assessed on the basis of the sequence of 456 base pairs of the mitochondrial cytochrome b genes. The resultant phylogenetic trees indicate that the R.tagoi group with distinct breeding habits, differentiated earliest, followed by R.tsushimensis and R.okinavana, both isolated on islands. Considerable genetic differentiation is found between subspecies of R.tagoi and between two populations of R.okinavana. Species with 2n=24 chromosomes form a monophyletic group, and appear to be derived from a 26-chromosome ancestor.Second, phylogenetic relationships of brown frogs with 2n=24 chromosomes are analyzed using topotypic R.chensinensis from Tsingling, China. The result indicated that R.ornativentris, R.pirica or R.dybowskii, all once regarded as conspecific with R.chensinensis was found to be distinct from R.chensinensis from the type locality not only morphologically but also genetically.DNA sequences of major brown frog taxa from China, Taiwan, and Russia have already been determined, and analyzes of their phylogenetic relationships are now under way. Further, phylogenetic relationships of representative ranid genera from Southeastern Asia has also been studied, and by accumulating these data and comparing the result with morphological and ecological information, relationships of the family Ranidae as a whole would be made clear and limit of biochemical methods would be elucidated.

本研究是作为自1920年以来没有人成功的Ranidae家族系统发育分析的一部分，通过比较DNA序列的新方法来澄清相关分类群之间的关系。首先，基于线粒体细胞色素b基因的456个碱基对序列，对已知的10个日本褐蛙（Rana pirica、R.dybowskii、r.d ornativentris、r.d japonica、r.t agooi tagoi、r.t yakushimensis、r.t okiensis、r.t sakuraii、r.t tsushimensis和r.k okavana）的系统发育关系进行了评价。系统发育树结果表明，具有不同繁殖习性的R.tagoi类群分化最早，tsushimensis类群和r.t okinavana类群分化最晚，均为孤岛类群。在塔古依的亚种之间和两个居群之间发现了相当大的遗传分化。具有2n=24条染色体的物种形成单系群，似乎来自26条染色体的祖先。其次，对2n=24条染色体的棕蛙进行系统发育分析。结果表明，曾被认为与中华r.r . sinsinensis同源的r.r ornativentris、r.r .pirica和R.dybowskii在形态上和遗传上都与中国r.r . sinensis有明显的差异。来自中国、台湾和俄罗斯的主要褐蛙类群的DNA序列已经确定，它们的系统发育关系的分析正在进行中。此外，本文还对东南亚地区具有代表性的Ranidae属的系统发育关系进行了研究，通过收集这些资料，并将结果与形态学和生态学资料进行比较，以明确Ranidae科的整体关系，并阐明生化方法的局限性。