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Studies on the diversity of graminicolous Helminthosporium species by DNA analyzes and its taxonomical application

DNA分析研究禾本科长蠕孢属物种多样性及其分类学应用

基本信息

批准号：
07456029
负责人：
TSUDA Mitsuya
金额：
$ 5.06万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456029/
关键词：
Bipolaris Base sequence melanin synthetic gene Brn1 gene Cochliobolus heterostrophus gene analyzes Helminthosporium plant pathogenic fungi Curvularia Drechslera Exserohilum トウモロコシごま葉枯病菌メラニン生合成 Brnl遺伝子系統関係 Curvularia genicul

项目摘要

The melanin biosynthetic pathway of Cochliobolus heterostrophus was analyzed. The Brn1 gene coding for reductase gene involved in melanin biosynthesis was cloned and sequenced. The Brn gene contains one open reading frame (804bp), consisting of 3 exons separated by two introns, and the predicted Brn1 polypeptide consists of 267 amno acids. The gene was contained only one copy per genome in this fungus.By using this gene, the taxonomical relationships in so-called graminicolous Helminthosporium species, Bipolaris, Curvularia, Drechslera, Exserohilum were analyzed. The Brn1 gene of some selected members in four genera was amplified with partial sequences derived from that of Cochliobolus heterostrophus as primers in PCR anc sequenced. Alignment of these sequences showed high similarities to each other through the entire sequence but intron regions.Neibour joining analysis using these sequences constructed four clusters. The cladogram well reflected the differentiation in this gene within these genera. However, relationships within these four genera differ from thosew based on morphological characters.Some other related studies on the plant pathogenic fungi were also summarized here.

对异柄旋孢霉的黑色素生物合成途径进行了分析。克隆并测序了编码黑色素生物合成中还原酶基因的Brn1基因。Brn基因含有一个开放阅读框（804 bp），由3个外显子和2个内含子组成，预测的Brn 1多肽由267个氨基酸组成。利用该基因分析了禾生长蠕孢菌属（Helminthosporium）、平脐蠕孢属（Bipolaris）、弯孢属（Curvularia）、德氏弯孢属（Drechslera）和突脐蠕孢属（Exserohilum）的分类学关系。以异柄旋孢菌Brn1基因部分序列为引物，对4个属部分成员的Brn1基因进行了PCR扩增和序列测定。这些序列的比对结果表明，除内含子区域外，其它区域的序列都具有很高的相似性。该基因在各属中的分化程度较好地反映了该基因在各属中的分化。但这四个属之间的亲缘关系与形态学上的关系不同，本文还对植物病原真菌的其他相关研究进行了综述。