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Developmental and comparative studies of the glial complex in white matter

白质胶质复合体的发育和比较研究

基本信息

批准号：
07457006
负责人：
SUZUKI Mitsuru
金额：
$ 0.64万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457006/
关键词：
regeneration hippocampus fimbria white matter glia oligodendrocyte astrocyte microglia 乏宅起細胞アポトーシス

项目摘要

The ultrastructure of developing oligodendrocytes in the interfascicular glial complex was examined by means of the criteria of Mori and Leblond (JCN 139 : 1-30,1970). The time course of myelination as related to the maturation of the oligodendrocytes was also investigated. Horizontal and transverse sections of fimbria were prepared for both electron microscopic and immunohistochemical studies. Few scattered glioblasts at PO became transformed into longer unicellular rows by P7, and the glial rows mainly consisted of light oligodendrocytes at P7. Medium oligodendrocytes became dominant in the glial rows at P28. Dark oligodendrocytes appeared at P28 and increased until P60 when the fimbria had an almost adult form with densely packed dark oligodendrocytes. Heterogeneity of the interfascicular glial rows was particularly prominent, and in addition unclassified intermediate types of oligodendrocytes were often observed from P14 to P28. Both mitoses and apoptosis in the glial rows were con … More firmed at the electron microscope level. Some of the apoptotic glia were immunohistochemically proved to be astrocyte. Glial interactions such as Thymosin-positive microglia surrounding apoptotic cells and astrocytic processes with direct contact to adjacent oligodendrocytes were observed. Myelinated fimbrial fibers were identified at P10, and the average number of laminae of the myelinated fibers significantly increased about three times from P10 to P60 (p<0.01). The rate of myelinating fibers among the whole fibers also significantly increased from 14.5% (P14) to 71.5% (P60) (p<0.01). In the multifocal developing pattern of the glial complex, oligodendrocytes seemed to mature continuously and independently repeating mitoses and apoptosis in each glial focus. We also investigated axon-glia interactions using scanning electron microscope and confocal microscope. Theoretical 'woven' meshwork of axons and glial processes in the central white matter was confirmed by the scanning electron microscopic observation. Less

用Mori和Leblond（JCN 139：1- 30，1970）的标准检查束间神经胶质复合体中发育中的少突胶质细胞的超微结构。还研究了与少突胶质细胞成熟相关的髓鞘形成的时间过程。制备海马伞的水平和横切面，用于电镜和免疫组化研究。P7时，少量散在的胶质母细胞转化为较长的单细胞行，P7时胶质行主要由浅色少突胶质细胞组成。P28时，中少突胶质细胞在胶质细胞行中占优势。在P28时出现深色少突胶质细胞，并且增加直到P60时，伞具有几乎成年的形式，其中密集地填充深色少突胶质细胞。束间胶质细胞行的异质性是特别突出的，此外，未分类的中间类型的少突胶质细胞经常观察到从P14到P28。神经胶质细胞的核分裂和凋亡均呈阳性， ...更多信息在电子显微镜水平上得到证实。部分凋亡的胶质细胞经免疫组化证实为星形胶质细胞。观察到胶质细胞相互作用，如凋亡细胞周围的胸腺肽阳性小胶质细胞和与相邻少突胶质细胞直接接触的星形胶质细胞突起。P10时有髓菌毛纤维出现，P10 ~ P60时有髓纤维的板层数明显增加约3倍（p<0.01）。有髓纤维占总纤维的比例也由14.5%（P14）增加到71.5%（P60）（P<0.01）。在胶质复合体的多灶性发育模式中，少突胶质细胞似乎是连续成熟的，并且在每个胶质灶中独立地重复有丝分裂和凋亡。我们还利用扫描电镜和共聚焦显微镜研究了轴突-胶质细胞的相互作用。通过扫描电镜观察证实了轴突和神经胶质突起在中央白色物质中的理论“编织”网络。少