ヒト顎関節円板における酸性ムコ多糖体の局在・分布の解析

人颞下颌关节盘中酸性粘多糖的定位和分布分析

• 批准号: 07457483
• 负责人: 柴田 考典
• 金额: $ 3.33万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457483/
• 关键词: ヒト; 顎関節円板; 酸性ムコ多糖体; 顎関節円板転位; ヒアルロン酸; コンドロイチン硫酸; ケラタン硫酸

前方転移に伴う顎関節円板の変性過程を明らかにするため、まず変性過程のゴールとしての関節円板の石灰化に着目した。ヒト顎関節円板の石灰化には少なくとも二つの機序が示唆されることを第7回日本顎関節学会総会において報告した。すなわち穿孔の見られた関節円板では、穿孔部に隣接した関節円板下面に軟骨様骨が形成されていた(骨化)。一方、穿孔の見られなかった関節円板では後方肥厚部と中央狭窄部との移行部の中層において膠原線維の走向の乱れ、硝子様変性および軟骨細胞の集蔟を認め、その部位に石灰化が生じていた(異所性石灰化)。そこで、後者の穿孔の見られない関節円板における変性過程をさらに追究するため、学生解剖実習屍体より剖出した245顎関節円板のうち、前方転位が見られ、かつ穿孔を伴わず、また軟X線写真にて明かな石灰化の見られない8個体16円板を対象とした。まず、関節円板を内外側正中で矢状断し、内側半分を標本とした。標本を通法に従いパラフィン包埋し、関節円板正中矢状面中央から内側に向かって厚さ5μmで各標本につき200枚の連続矢状断切片を作成し、H-E、トルイジンブルーpH2.5,4.1,7.0染色、抗ケラタン硫酸抗体を用いたABC法による免疫染色を施し、病理組織学的に検討した。その結果、2標本(12.5%)において顎関節円板の後方肥厚部から中央狭窄部の移行部の中層に、膠原線維の走向の乱れ、かつトルイジンブルーpH4.1,7.0および抗ケラタン硫酸抗体にも濃染する部分が認められた。同部位は既報において異所性石灰化が認められた領域とほぼ同一部分と考えられ、顎関節円板の変性過程において石灰化に先立つ変化の1つとして、硫酸化GAGの集積が起こっていることを示すものと言える。なお、1標本においては円板後方肥厚部の下面にもトルイジンブルーpH4.1,7.0および抗ケラタン硫酸抗体に濃染する領域が見られた。

The front of the jaw joint plate changes the process of the jaw joint plate. The mechanism of calcification of jaw joint plate was presented in the 7th Japan Jaw Joint Society Conference. Cartilaginous bone is formed below the articular plate adjacent to the perforation. A side, perforation and joint plate, posterior thickening part, central narrowing part, middle layer of transition part, collagen trace direction, nitrate-like property, chondrocyte aggregation, calcification of all parts (heterogeneous calcification). For example, students dissected cadavers, dissected 245 jaw joint plates, observed anterior position, perforated holes, and soft X-ray photographs of calcified cadavers, 8 individual cadavers and 16 cadavers were imaged. The joint plate is cut in the middle of the medial and lateral sides, and the medial half is cut in the middle of the medial and lateral sides. 200 consecutive sagittal sections of each specimen were prepared from the medial part of the median sagittal plane of the articular plate, 5 μ m thick, by the method of liguid penetration, H-E staining, anti-liguid sulfuric acid antibody immunostaining with ABC method, and histopathological examination. The results showed that 2%(12.5%) of the samples were found in the middle layer of the posterior thickening part of the mandibular joint plate, the middle layer of the transitional part of the central narrowing part, the disorder of the collagen trace, the pH of the collagen trace, the pH of 4.1, 7.0, and the concentration of anti-Cisarino sulfuric acid antibody. The same part of the jaw joint plate is divided into two parts: the first part of the jaw joint plate is divided into two parts: the first part of the jaw joint plate is divided into three parts: the first part of the jaw joint plate is divided into three parts: the first part of the jaw joint plate is divided into three parts: the first part of the jaw joint plate is divided into three parts: the first part of the jaw joint plate is divided into four parts: the second part of the jaw joint plate is divided into four parts: the first part of the jaw joint plate is divided into three parts: the second part of the jaw joint plate is divided into four parts: the third part of the jaw joint plate is divided into four parts: the third part of the jaw joint plate is divided into four parts: the fourth part of the jaw joint plate is divided into four parts: the third part of the jaw joint plate is divided into four parts: the fourth part of the jaw joint plate is divided into four parts: the third part of the jaw joint plate is divided into four parts: the first part of the jaw joint plate is divided into four parts; the fourth part of the jaw joint plate is divided into four The following areas of pH4.1, 7.0 and anti-H2SO4 antibody concentration were found in the posterior thickening of the plate.

项目成果

Takanori Shibata: "Mechanisms of Calcification in Human TMJ Discs" J.of Oral and Maxillofacial Surgery. 55. (1997)

Takanori Shibata：“人类颞下颌关节盘钙化机制”J.of Oral and Maxillofacial Medicine。

Takanori Shibata: "Liocaligation of GAG components in the human displaced Discs" Journal of Dental Research. 75. (1996)

Takanori Shibata：“人类移位椎间盘中 GAG 成分的定位”《牙科研究杂志》。