Creation of Novel Macrocyclic Compounds that Selectively Recognize Nucleobases to Control Gene Expression

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19951)Several di- and trinuclear macrocyclic polyamine complexes have been synthesized, which recognize specific nucleotides (uridien, Thymidine) in oligomers, and barbital. The jstrong interactions were studied by pH-metric titrations and NMR and UV spectroscopic methods.2)A novel polyamine cryptate was synthesized, whose dizinc complex selectively cleaves phosphomonoesters. Intrinsic zinc prooperties in alkaline phosphatase are disclosed.3)A new macrocyclic polyamine-terbium complex waas synthesized and its crystal structure was determined by X-ray study. This complex selectively recognizes guamine among nucleobases by emitting fluorescence.19961)The newly synthesized bis (Zn^<II>-cyclen) complexes were shown to possess very strong anti-HIV activity in vitro.2)The newly synthesized Zn^<II>-cyclen complexes denature poly A-poly U and poly dA-poly dT at rom temperature. These are the first examples of small molecules that effectively modify DNA double strands.3)A New biomimetic zinc fluorophore, dansyl-pendant cyclen, was synthesized, which has a great potential for analysis of trace zinc in cells.4)A new cyclen attached with a long alkyl chain was synthesized. Its zinc complex is one of the best catalysis to detoxify nerve gases like salin.