Exo utero gene introduction to specific organs and tissues during fetal and neonatal period
将子宫外基因引入胎儿和新生儿时期的特定器官和组织
基本信息
- 批准号:07507007
- 负责人:
- 金额:$ 6.14万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (A)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Exo utero development system and techniques of microinjection into fetuses have been improved. To increase efficiency of the uptake of exogenous genes injected into mouse embyonic organs, optimal conditions of gene carriers and electroporation have been established, and usefulness of antisense oligonucleotides has been estimated. To demonstrate the potential of exo utero system, several biologically active substances were injected into the specific fetal organs, and their effects were analyzed. The exo utero experimental systems to analyze the histogenesis of several organs have been established.1. Improvement of exo utero system : Reduction of manipulation time for each procedure, modification of incision of the uterine wall, anesthesia and resuscitation, all together have significantly improved the survival rate after the manipulation and efficiency of microinjection.2. Optimization of conditions for gene introduction : Among several liposomes, the ones with the highest efficiency of … More reporter gene incorporation under different conditions were determined. Conditions of electroporation with the BTX system were evaluated and the flat type electrode showed an excellent handling. Conditions have been established for good diffusion of injected solution into the cerebral parenchyme with minimum damage to the tissue.3. Introduction of antisense oligonucleotides : Antisense oligonucleotides for Tag1 and Mab21 was injected into the fetal cerebral ventricle at single-dose or double-dose with an interval, and the protein expression levels decreased significantly.4. Application of exo utero system into the functional analysis of regulatory molecules in the histogenesis of the organs : LIF was injected into the cerebral ventricle and showed specific effects on the cerebral cortex including increase in the neuroblasts and fibers in the specific cortical layrs. The effects of ACTH on the fetal adrenal were similarly evidenced by the established exo utero experimental system. The exo utero experimental systems for analysis of pathogenesis of hydrocephaly and excencephaly, and development of the inner ear and joints have also been established. Less
子宫外发育系统和胚胎显微注射技术不断完善。为了提高外源基因注入小鼠胚胎器官的效率,已建立了基因载体和电穿孔的最佳条件,并已估计反义寡核苷酸的有用性。为了证明子宫外系统的潜力,将几种生物活性物质注射到特定的胎儿器官中,并分析它们的作用。建立了分析几种器官组织发生的子宫外实验系统.子宫外系统的改进:减少每一次操作时间,改进子宫壁切口,麻醉和复苏,显著提高了操作后的存活率和显微注射的效率.基因导入条件的优化:在几种脂质体中, ...更多信息 测定不同条件下报告基因的掺入。评价了用BTX系统进行电穿孔的条件,并且平板型电极显示出优异的操作性。已经建立了注射溶液良好扩散到脑实质中的条件,对组织的损伤最小。反义寡核苷酸的导入:将Tag1和Mab21的反义寡核苷酸以单剂量或双剂量间隔注射到胎鼠脑室,蛋白表达水平显著降低.子宫外系统在器官组织发生调控分子功能分析中的应用:将LIF注入脑室,对大脑皮层表现出特异性作用,包括增加特定皮层层的神经母细胞和纤维。ACTH对胎儿肾上腺的影响也被已建立的子宫外实验系统所证实。建立了分析脑积水和无脑畸形发病机制及内耳和关节发育的子宫外实验系统。少
项目成果
期刊论文数量(75)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
J.Hatta: "Experimental hydrocephalus : history of animal models and the application of deuterium oxide to treatment." Jpn.J.Deuterium Sci.6. 13-19 (1997)
J.Hatta:“实验性脑积水:动物模型的历史和氧化氘在治疗中的应用。”
- DOI:
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- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
S.Matsuda: "Apoptosis in the development of the temporomandibular joint." Anat.Embryol.196. 383-391 (1997)
S.Matsuda:“颞下颌关节发育中的细胞凋亡。”
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
森山 賢治: "ノックアウトマウスデータブック(Molecular Medicinc 臨時増刊)" 中山書店, 564 (1997)
Kenji Moriyama:“Knockout Mouse Data Book(分子医学额外版)”中山书店,564(1997)
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- 影响因子:0
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R.Hashimoto: "Selective translocation of different markers in the ante- and retrograde pathways between the Golgi apparatus and the rough endoplasmic reticulum in a hybridoma cell line." Ann.Anat.179. 105-116 (1997)
R.Hashimoto:“杂交瘤细胞系中高尔基体和粗面内质网之间的前行和逆行途径中不同标记的选择性易位。”
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- 影响因子:0
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- 通讯作者:
Koera,K.: "K-ras in essential for the development of the mouse embryo." Oncogene. 15. 1151-1159 (1997)
Koera,K.:“K-ras 对于小鼠胚胎的发育至关重要。”
- DOI:
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- 影响因子:0
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OTANI Hiroki其他文献
OTANI Hiroki的其他文献
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{{ truncateString('OTANI Hiroki', 18)}}的其他基金
Fundamental research on students performance for solving problem statistically
学生统计解决问题表现的基础研究
- 批准号:
19K20947 - 财政年份:2018
- 资助金额:
$ 6.14万 - 项目类别:
Grant-in-Aid for Research Activity Start-up
Mechanisms of abnormal organogenesis and histogenesis of the kidney and ureter and development of the embryonic treatment
肾脏和输尿管异常器官发生和组织发生机制及胚胎治疗的发育
- 批准号:
22659289 - 财政年份:2010
- 资助金额:
$ 6.14万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Developmental analysis of neuro-immuno-endocirne network and harmonized histogenesis of organs
神经-免疫-内分泌网络和器官协调组织发生的发育分析
- 批准号:
15209034 - 财政年份:2003
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$ 6.14万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Establishment of "In situ Knock-out" method
“原位敲除”方法的建立
- 批准号:
13557002 - 财政年份:2001
- 资助金额:
$ 6.14万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of expression and function of regulatory molecules in the whole embryos by new developmetnal engineering system
新型发育工程系统分析全胚胎调控分子表达及功能
- 批准号:
08307001 - 财政年份:1996
- 资助金额:
$ 6.14万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Systemic pattern of apoptosis in human embryos and establishment of mouse culture system to analyze its control mechanisms
人胚胎细胞凋亡的系统模式及小鼠培养体系的建立分析其调控机制
- 批准号:
06807003 - 财政年份:1994
- 资助金额:
$ 6.14万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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