Novel application of small domestic herbivora raised by discarded recycling resources

废弃回收资源培育小型家养食草动物的新用途

基本信息

批准号：
07556129
负责人：
MANABE Noboru
金额：
$ 2.82万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

A novel application method of small domestic herbivora raised by discarded recycling resources was developed. Moreover, highly sensitive method to detect the environmental toxicants which induce genetic and teratogenic toxicity to domestic mammals and human was developed. The new assessment method realized non destructive and real time detection. In vivo and in vitro phosphorus-31 and proton nuclear magnetic resonance (NMR) spectra of the intestinal tracts including liver and reproductive organs, ovary and testis, and embryo of experimental and domestic herbivora were acquired using a house-made surface coil, conventional round coil, and/or imaging coils. Beta-ATP/inorganic phosphate ratio which represents the high energy state and intracellular pH etc.were observed in vivo. Many environmental toxicants were identified and quantified without any extraction processes from these organs and embryos using NMR spectroscopy. Proton NMR experiments were done by a modification of conventional … More techniques, and 31P NMR experiments were performed as follows : During the NMR examination, the sample was maintained in a probe chamber at controlled air temperature of 25C.In vivo 31P NMR spectra of the steady-state organs within intact animals were obtained using a JNM-alpha-400 FT-NMR spectrometer (JEOL) equipped with 89-mm bore superconducting magnet (9.20 Tesla ; Oxford Instruments). The animals were mounted on a surface coil probe (two-turns, 2.0 cm diameter) which was tuned to 31P at 161.7 MHz and used in conjunction with a ferrite screen composed of strips of computer tape to eliminate 31P NMR signals arising from superficial muscles. Field homogeneity was controlled by adjusting shim parameters using a water 1H signal derived from the organ sample. Acquisition conditions were set as follows to obtain fully relaxd signals : the pulse width was 20 micro-seconds, the pulse repetition time was 3.0 s, and 200 scans were accumulated. Thus, it took 5-25 min for each measurement. After exponential multiplication, the accumulated free induction decays were subjected to Fourier transformation. Individual spectral peak areas were calculated by computer integration with JEOL software, and the creatine phosphate peak was used as a positional reference. The relative amount of each compound was converted to absolute concentration by comparison with the data of an external reference (20mM methylene diphosphonate in water) in a spherical bulb. The present study showed that NMR spectroscopy is a novel assessment tools for digestive capacity of small domestic herbivora and for evaluation of reproductive and genetic toxicity in domestic mammals. Less

开发了一种新型的用于废弃回收资源饲养的小型家庭赫尔比瓦拉的应用方法。此外，开发了高度敏感的方法来检测诱导遗传和致致致造性毒性对家庭哺乳动物和人类的遗传毒性的环境有毒物质。新的评估方法实现了非破坏性和实时检测。肠道和体外磷-31和质子核磁共振（NMR）光谱，包括肝脏和生殖器官，卵巢和睾丸，卵巢和睾丸，实验性和家用Herbivora的胚胎。在体内观察到代表高能状态和细胞内pH等的β-ATP/无机磷酸盐比。使用NMR光谱法鉴定出许多环境有毒物质，而没有从这些器官和胚胎中提取任何提取过程。质子NMR实验是通过修改常规……更多技术和31p NMR实验进行的，如下：在NMR检查过程中，使用Intacter在Intact Artift Antive Artift Antergantim of Intacter动物的Vivo 31p NMR光谱中，将样品保持在探头室中，使用Intacter的31p NMR光谱使用jnm-400 jnm-alperpecs clect of jnm-400 fttriptrip。带有89毫米的孔超导磁铁（9.20 Tesla；牛津仪器）。将动物安装在表面线圈探头（两转，直径2.0 cm）上，该探针以161.7 MHz调节为31p，并与由计算机胶带的条带一起使用，以消除由31p NMR信号组成，从而消除了由浅表肌肉引起的31p NMR信号。通过使用从器官样品衍生的水信号来调整垫片参数来控制场均匀性。设置采集条件如下设置以获得完全放松的信号：脉冲宽度为20个微秒，脉冲重复时间为3.0 s，并积累了200张扫描。那就是每个测量需要5-25分钟。指数乘法后，累积的自由感应衰减经过傅立叶变换。通过与JEOL软件的计算机集成来计算各个光谱峰区域，并将创建磷酸盐峰用作位置参考。通过与球形灯泡中外部参考的数据（水中20mm甲基二膦酸盐）的数据进行比较，将每种化合物的相对量转化为绝对浓度。本研究表明，NMR光谱是一种新颖的评估工具，可用于小型家用赫尔比鸟的消化能力以及评估家用哺乳动物中生殖和遗传毒性的评估。较少的