Regulation mechanisms in granulosa cell apoptosis in follicular atresia

卵泡闭锁颗粒细胞凋亡的调控机制

• 批准号: 07660379
• 负责人: MANABE Noboru
• 金额: $ 1.41万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660379/
• 关键词: apoptosis; granulosa cells; cumulus cells; oocytes; ovarian follicular atresia; proteases; monoclonal antibodies; cell membrane glycoprotein-receptors; 卵胞選択的死滅; プログラム細胞死・アポトーシス; ブタ卵巣; TUNEL組織化学; レシチン細胞化学; 細胞膜結合糖鎖

The porcine antral follicles were dissected from the ovaries of mature pigs, and then granulosa and cumulus cells were isolated from each follicle. In atretic follicles, high activity of neutral Ca2+/Mg2+-dependent endonuclease and DNA ladder formation estimated by electrophoresis were noted only in granulosa cells but not in cumulus cells. Extremely low activity of the endonuclease and no DNA ladder formation were observed in both types of cells obtained from healthy follicles. Moreover, apoptotic cells were observed histochemically among granulosa cells only. A good correlation between the endonuclease activity of granulosa cells and the progesterone/estradiol ratio of follicular fluid in each follicle was found. These results suggest that apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pigs.

从成熟猪卵巢中分离出有腔卵泡，分离出颗粒细胞和卵丘细胞。在闭锁卵泡，高活性的中性Ca ~（2+）/Mg ~（2+）依赖性核酸内切酶和DNA梯状条带的形成估计电泳只注意到颗粒细胞，而不是在卵丘细胞。在从健康卵泡获得的两种类型的细胞中均观察到极低的核酸内切酶活性和无DNA梯状条带形成。此外，组织化学上仅在颗粒细胞中观察到凋亡细胞。颗粒细胞的核酸内切酶活性与卵泡液中孕酮/雌二醇比值之间存在良好的相关性，提示在闭锁的有腔卵泡中，颗粒细胞发生凋亡，而卵丘细胞不发生凋亡。

项目成果

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Nagano N. 等人：“顺铂诱导的非少尿性急性肾功能衰竭大鼠对心房钠尿肽 (ANP) 的肾脏反应”《兽医医学科学杂志》。

眞鍋 昇ら: "Effect of the mycelial extract of cultured cordyceps sinensis onin vivo hepatic energy metabolism in the mouse." Journal of Pharmacology. 70. 85-88 (1996)

Noboru Manabe 等人：“培养冬虫夏草菌丝体提取物对小鼠体内肝脏能量代谢的影响。药理学杂志 70. 85-88 (1996)”

Noboru Manabe, Yukinobu Kuribayashi, Makoto Miyamoto, Keiko kuramitsu, Yasuyoshi Azuma, Yoshihiro Furuya, Nobuo Nagano, and Hajime Miyamoto.: "Immunohistochemical quantification of fast-myosin in frozen histological sections of goathind limb muscles." Ani

Noboru Manabe、Yukinobu Kuribayashi、Makoto Miyamoto、Keiko kuramitsu、Yasuyoshi Azuma、Yoshihiro Furuya、Nobuo Nagano 和 Hajime Miyamoto.：“山羊四肢肌肉冷冻组织切片中快速肌球蛋白的免疫组织化学定量。”

Noboru Manabe, Yuzuru Imai, Yoshihiro Kimura, Akira Myoumoto, Miki Sugimoto, Hajime Miyamoto, Yoshinori Okamura, and Manabu Fukumoto.: "Ca2+/Mg2+-dependent endonuclease but not ca2+Dependent, mg2+-Dependent or cation-independent endonuclease is involved i

Noboru Manabe、Yuzuru Imai、Yoshihiro Kimura、Akira Myoumoto、Miki Sugimoto、Hajime Miyamoto、Yoshinori Okamura 和 Manabu Fukumoto。：“Ca2 / Mg2 依赖性核酸内切酶，但不涉及 ca2 依赖性、mg2 依赖性或阳离子非依赖性核酸内切酶

Noboru Manabe, Yasuyoshi Azuma, Yukinobu Kuribayashi, Makoto Miyamoto, Keiko Kuramitsu, Yoshihiro Furuya, Nobuo Nagano, and Hajime Miyamoto.: The 8th Animal Science Congress : Immunohistochemical quantification of fast-myosin in frozen histological sectio

Noboru Manabe、Yasuyoshi Azuma、Yukinobu Kuribayashi、Makoto Miyamoto、Keiko Kuramitsu、Yoshihiro Furuya、Nobuo Nagano 和 Hajime Miyamoto.：第八届动物科学大会：冷冻组织学切片中快速肌球蛋白的免疫组织化学定量