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Regulation mechanisms in granulosa cell apoptosis in follicular atresia

Regulation mechanisms in granulosa cell apoptosis in follicular atresia
卵泡闭锁颗粒细胞凋亡的调控机制
批准号:
07660379
负责人:
MANABE Noboru
金额:
$1.41万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1995
资助国家:
日本
项目状态:
已结题
起止时间:
1995 至 1996

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中文摘要
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英文摘要
The porcine antral follicles were dissected from the ovaries of mature pigs, and then granulosa and cumulus cells were isolated from each follicle. In atretic follicles, high activity of neutral Ca2+/Mg2+-dependent endonuclease and DNA ladder formation estimated by electrophoresis were noted only in granulosa cells but not in cumulus cells. Extremely low activity of the endonuclease and no DNA ladder formation were observed in both types of cells obtained from healthy follicles. Moreover, apoptotic cells were observed histochemically among granulosa cells only. A good correlation between the endonuclease activity of granulosa cells and the progesterone/estradiol ratio of follicular fluid in each follicle was found. These results suggest that apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pigs.
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Nagano N. et al.: "Renal responses to atrial natriuretic peptide(ANP) in rats with non-oliguric acute renal failure induced by cisplatin" The Journal of Veterinary Medical Science. 57. 997-1002 (1995)
Nagano N. 等人:“顺铂诱导的非少尿性急性肾功能衰竭大鼠对心房钠尿肽 (ANP) 的肾脏反应”《兽医医学科学杂志》。
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眞鍋 昇ら: "Effect of the mycelial extract of cultured cordyceps sinensis onin vivo hepatic energy metabolism in the mouse." Journal of Pharmacology. 70. 85-88 (1996)
Noboru Manabe 等人:“培养冬虫夏草菌丝体提取物对小鼠体内肝脏能量代谢的影响。药理学杂志 70. 85-88 (1996)”
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Noboru Manabe, Yukinobu Kuribayashi, Makoto Miyamoto, Keiko kuramitsu, Yasuyoshi Azuma, Yoshihiro Furuya, Nobuo Nagano, and Hajime Miyamoto.: "Immunohistochemical quantification of fast-myosin in frozen histological sections of goathind limb muscles." Ani
Noboru Manabe、Yukinobu Kuribayashi、Makoto Miyamoto、Keiko kuramitsu、Yasuyoshi Azuma、Yoshihiro Furuya、Nobuo Nagano 和 Hajime Miyamoto.:“山羊四肢肌肉冷冻组织切片中快速肌球蛋白的免疫组织化学定量。”
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Noboru Manabe, Yuzuru Imai, Yoshihiro Kimura, Akira Myoumoto, Miki Sugimoto, Hajime Miyamoto, Yoshinori Okamura, and Manabu Fukumoto.: "Ca2+/Mg2+-dependent endonuclease but not ca2+Dependent, mg2+-Dependent or cation-independent endonuclease is involved i
Noboru Manabe、Yuzuru Imai、Yoshihiro Kimura、Akira Myoumoto、Miki Sugimoto、Hajime Miyamoto、Yoshinori Okamura 和 Manabu Fukumoto。:“Ca2 / Mg2 依赖性核酸内切酶,但不涉及 ca2 依赖性、mg2 依赖性或阳离子非依赖性核酸内切酶
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29
    Evaluation method for quality the farm animal egg: Based on the molecular mechanism of follicle selection
    Rescue of potential oocytes in livestock ovaries by artificial control of apoptosis inhibitor
    • 批准号:
      24658231
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.58万
    • 财政年份:
      2012
    • 负责人:
      MANABE Noboru
    • 依托单位:
    Creation of judging method for livestock egg quality based on the molecular mechanism of follicular selection
    • 批准号:
      22380148
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $12.73万
    • 财政年份:
      2010
    • 负责人:
      MANABE Noboru
    • 依托单位:
    Creation of rescue system for potential oocytes by apoptosis inhibitory factor gene transfer and xenotransplantation techniques
    • 批准号:
      21658092
    • 项目类别:
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额:
      $2.18万
    • 财政年份:
      2009
    • 负责人:
      MANABE Noboru
    • 依托单位:
    海外基金