Histochemical Microquantitation for Extracellular Matrix Proteins and mRNAs in Mammalian Histologic Sections

哺乳动物组织学切片中细胞外基质蛋白和 mRNA 的组织化学微量定量

• 批准号: 05660344
• 负责人: MANABE Noboru
• 金额: $ 1.28万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05660344/
• 关键词: Extracellular matrix; Glomerulonephritis; Histochemical microquantitation; Chronic viral hepatitis; Hepatic fibrosis; Experimental animal models; Skeletal myofibers; Isomyosins; 腎疾患モデル; インターフェロンα; 慢性肝炎; 骨格筋; 速収縮ミオシン

Extracellular matrix (ECM) proteins and mRNAs in frozen histologic sections were quantified by a histochemical microquantitation methods, which are developed in the present research project. For instance, ECM contents in renal cortex of rats with experimental glomerulonephritis and in liver from the patients with viral hepatitis were measured. The increase in ECM contents correlated well with conventional biochemical parameters for renal disorders. The immunohistochemical micromethod may make it possible to evaluate the histopathological diagnosis of mesangial proliferative glomerulonephritis quantitatively. This novel histochemical microquantitation technique is able to use for quantification of other biological substances, which plays an important role in the cellular physiological functions. For example, fast-myosin in frozen histological sections was quantified by a histochemical micromethod. Fast-myosin levels in mammalian skeletall muscles were quantified by the microtechnique. In conclusion, the micromethod is sensitive enough to measure the content of ECM proteins and mRNAs in the small sections, and make it possible to quantify the ECM levels in the biopsy sections. The micromethod is useful for histopathological diagnosis of the diseases, for precise evaluation of physiological and pathological characteristics of the organs, and for estimation of therapeutic efficacy of drugs.

通过本研究项目开发的组织化学微量定量方法对冷冻组织切片中的细胞外基质（ECM）蛋白和mRNA进行定量。例如，测定了实验性肾小球肾炎大鼠肾皮质和病毒性肝炎患者肝脏中的ECM含量。ECM含量的增加与肾脏疾病的常规生化参数相关。免疫组化显微方法可定量评价系膜增生性肾小球肾炎的组织病理学诊断。这种新的组织化学微量定量技术可以用于其他生物物质的定量，这些生物物质在细胞生理功能中起着重要作用。例如，快速肌球蛋白在冷冻组织切片定量组织化学微量法。通过显微技术定量哺乳动物骨骼肌中的快肌球蛋白水平。总之，微量法足够灵敏，可以测量小切片中ECM蛋白和mRNA的含量，并可以定量活检切片中的ECM水平。该方法可用于疾病的组织病理学诊断，精确评价器官的生理和病理特征，并可用于药物疗效的评价。

项目成果

Nanabe N.et al.: "Immunohistochemical quantitation of extracellular matrices in the kidney cortex of rats with experimental nephropathy" International union of physiological sciences (0xford University Press,London,U.K), 256 (1993)

Nanabe N.等人：“实验性肾病大鼠肾皮质细胞外基质的免疫组织化学定量”国际生理科学联合会（0xford University Press，伦敦，英国），256（1993）

Manabe N.et al.: "Immunohistochemical microquantitaion method for type I collagen in kidney histologic section of the rats." Journal of Veterinary Medical Science. 56. 147-150 (1994)

Manabe N.等人：“大鼠肾脏组织切片中 I 型胶原蛋白的免疫组织化学微量定量方法。”

Manabe N.et al.: "Interferon-alpha 2b therapy reduces liver fibrosis in chronic non-A,non-B hepatitis : A quantitative histological evaluation." Hepatolory. 18. 1344-1349 (1993)

Manabe N.等人：“干扰素-α 2b 疗法可减少慢性非甲、非乙型肝炎的肝纤维化：定量组织学评估。”

Manabe N.et al: "Slow-twitch myofiber are observed in masseter muscle of the hypothalamic obese rat." Animal Science and Technology. 64. 440-447 (1993)

Manabe N.等人：“在下丘脑肥胖大鼠的咬肌中观察到慢肌纤维。”

Manabe N.et al.: "Immunohistochemical quantitation of extracellular matrices in the kidney cortex of rats with experimental nephropathy" International union of physiological sciences (Oxford University Press,London,U.K.), 253 (1993)

Manabe N.等人：“实验性肾病大鼠肾皮质细胞外基质的免疫组织化学定量”国际生理科学联合会（牛津大学出版社，伦敦，英国），253（1993）