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Histochemical Microquantitation for Extracellular Matrix Proteins and mRNAs in Mammalian Histologic Sections

哺乳动物组织学切片中细胞外基质蛋白和 mRNA 的组织化学微量定量

基本信息

批准号：
05660344
负责人：
MANABE Noboru
金额：
$ 1.28万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

Extracellular matrix (ECM) proteins and mRNAs in frozen histologic sections were quantified by a histochemical microquantitation methods, which are developed in the present research project. For instance, ECM contents in renal cortex of rats with experimental glomerulonephritis and in liver from the patients with viral hepatitis were measured. The increase in ECM contents correlated well with conventional biochemical parameters for renal disorders. The immunohistochemical micromethod may make it possible to evaluate the histopathological diagnosis of mesangial proliferative glomerulonephritis quantitatively. This novel histochemical microquantitation technique is able to use for quantification of other biological substances, which plays an important role in the cellular physiological functions. For example, fast-myosin in frozen histological sections was quantified by a histochemical micromethod. Fast-myosin levels in mammalian skeletall muscles were quantified by the microtechnique. In conclusion, the micromethod is sensitive enough to measure the content of ECM proteins and mRNAs in the small sections, and make it possible to quantify the ECM levels in the biopsy sections. The micromethod is useful for histopathological diagnosis of the diseases, for precise evaluation of physiological and pathological characteristics of the organs, and for estimation of therapeutic efficacy of drugs.

细胞外基质（Extracellular matrix， ECM）蛋白和mrna在冷冻组织切片中采用本研究项目开发的组织化学微定量方法进行定量。例如，测定实验性肾小球肾炎大鼠肾皮质和病毒性肝炎患者肝脏中的ECM含量。ECM含量的增加与肾脏疾病的常规生化参数有良好的相关性。免疫组织化学显微法可以定量评价系膜增生性肾小球肾炎的组织病理学诊断。这种新型的组织化学微量定量技术可用于其他在细胞生理功能中起重要作用的生物物质的定量。例如，用组织化学显微法定量冷冻组织切片中的快速肌球蛋白。采用显微技术定量测定了哺乳动物骨骼肌中的快速肌球蛋白水平。总之，微法足够灵敏，可以测量小切片中ECM蛋白和mrna的含量，并且可以量化活检切片中的ECM水平。显微方法可用于疾病的组织病理学诊断，准确评价器官的生理和病理特征，以及估计药物的治疗效果。