Histochemical Microquantitation for Extracellular Matrix Proteins and mRNAs in Mammalian Histologic Sections

哺乳动物组织学切片中细胞外基质蛋白和 mRNA 的组织化学微量定量

基本信息

  • 批准号:
    05660344
  • 负责人:
  • 金额:
    $ 1.28万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
  • 财政年份:
    1993
  • 资助国家:
    日本
  • 起止时间:
    1993 至 1994
  • 项目状态:
    已结题

项目摘要

Extracellular matrix (ECM) proteins and mRNAs in frozen histologic sections were quantified by a histochemical microquantitation methods, which are developed in the present research project. For instance, ECM contents in renal cortex of rats with experimental glomerulonephritis and in liver from the patients with viral hepatitis were measured. The increase in ECM contents correlated well with conventional biochemical parameters for renal disorders. The immunohistochemical micromethod may make it possible to evaluate the histopathological diagnosis of mesangial proliferative glomerulonephritis quantitatively. This novel histochemical microquantitation technique is able to use for quantification of other biological substances, which plays an important role in the cellular physiological functions. For example, fast-myosin in frozen histological sections was quantified by a histochemical micromethod. Fast-myosin levels in mammalian skeletall muscles were quantified by the microtechnique. In conclusion, the micromethod is sensitive enough to measure the content of ECM proteins and mRNAs in the small sections, and make it possible to quantify the ECM levels in the biopsy sections. The micromethod is useful for histopathological diagnosis of the diseases, for precise evaluation of physiological and pathological characteristics of the organs, and for estimation of therapeutic efficacy of drugs.
细胞外基质(Extracellular matrix, ECM)蛋白和mrna在冷冻组织切片中采用本研究项目开发的组织化学微定量方法进行定量。例如,测定实验性肾小球肾炎大鼠肾皮质和病毒性肝炎患者肝脏中的ECM含量。ECM含量的增加与肾脏疾病的常规生化参数有良好的相关性。免疫组织化学显微法可以定量评价系膜增生性肾小球肾炎的组织病理学诊断。这种新型的组织化学微量定量技术可用于其他在细胞生理功能中起重要作用的生物物质的定量。例如,用组织化学显微法定量冷冻组织切片中的快速肌球蛋白。采用显微技术定量测定了哺乳动物骨骼肌中的快速肌球蛋白水平。总之,微法足够灵敏,可以测量小切片中ECM蛋白和mrna的含量,并且可以量化活检切片中的ECM水平。显微方法可用于疾病的组织病理学诊断,准确评价器官的生理和病理特征,以及估计药物的治疗效果。

项目成果

期刊论文数量(50)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nanabe N.et al.: "Immunohistochemical quantitation of extracellular matrices in the kidney cortex of rats with experimental nephropathy" International union of physiological sciences (0xford University Press,London,U.K), 256 (1993)
Nanabe N.等人:“实验性肾病大鼠肾皮质细胞外基质的免疫组织化学定量”国际生理科学联合会(0xford University Press,伦敦,英国),256(1993)
  • DOI:
  • 发表时间:
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    0
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Manabe N.et al.: "Immunohistochemical microquantitaion method for type I collagen in kidney histologic section of the rats." Journal of Veterinary Medical Science. 56. 147-150 (1994)
Manabe N.等人:“大鼠肾脏组织切片中 I 型胶原蛋白的免疫组织化学微量定量方法。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Manabe N.et al.: "Interferon-alpha 2b therapy reduces liver fibrosis in chronic non-A,non-B hepatitis : A quantitative histological evaluation." Hepatolory. 18. 1344-1349 (1993)
Manabe N.等人:“干扰素-α 2b 疗法可减少慢性非甲、非乙型肝炎的肝纤维化:定量组织学评估。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Manabe N.et al: "Slow-twitch myofiber are observed in masseter muscle of the hypothalamic obese rat." Animal Science and Technology. 64. 440-447 (1993)
Manabe N.等人:“在下丘脑肥胖大鼠的咬肌中观察到慢肌纤维。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Manabe N.et al.: "Immunohistochemical quantitation of extracellular matrices in the kidney cortex of rats with experimental nephropathy" International union of physiological sciences (Oxford University Press,London,U.K.), 253 (1993)
Manabe N.等人:“实验性肾病大鼠肾皮质细胞外基质的免疫组织化学定量”国际生理科学联合会(牛津大学出版社,伦敦,英国),253(1993)
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  • 影响因子:
    0
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MANABE Noboru其他文献

MANABE Noboru的其他文献

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{{ truncateString('MANABE Noboru', 18)}}的其他基金

Evaluation method for quality the farm animal egg: Based on the molecular mechanism of follicle selection
基于卵泡选择分子机制的畜禽卵品质评价方法
  • 批准号:
    26292136
  • 财政年份:
    2014
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Rescue of potential oocytes in livestock ovaries by artificial control of apoptosis inhibitor
通过人工控制凋亡抑制剂拯救家畜卵巢中的潜在卵母细胞
  • 批准号:
    24658231
  • 财政年份:
    2012
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Creation of judging method for livestock egg quality based on the molecular mechanism of follicular selection
基于卵泡选择分子机制建立畜禽鸡蛋品质判断方法
  • 批准号:
    22380148
  • 财政年份:
    2010
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Creation of rescue system for potential oocytes by apoptosis inhibitory factor gene transfer and xenotransplantation techniques
凋亡抑制因子基因转移和异种移植技术构建潜在卵母细胞拯救体系
  • 批准号:
    21658092
  • 财政年份:
    2009
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of estimation method for mammalian oocyte quality based on molecular mechanism of follicular selection
基于卵泡选择分子机制的哺乳动物卵母细胞质量评价方法的建立
  • 批准号:
    18380164
  • 财政年份:
    2006
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Non-invasive assessment for teratogenic-toxicity of environmental disrupters by structural- and cel lurar-biological methods
通过结构生物学和细胞生物学方法对环境破坏物的致畸毒性进行非侵入性评估
  • 批准号:
    11556052
  • 财政年份:
    1999
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Novel application of small domestic herbivora raised by discarded recycling resources
废弃回收资源培育小型家养食草动物的新用途
  • 批准号:
    07556129
  • 财政年份:
    1995
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Regulation mechanisms in granulosa cell apoptosis in follicular atresia
卵泡闭锁颗粒细胞凋亡的调控机制
  • 批准号:
    07660379
  • 财政年份:
    1995
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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The elucidation of the role of HIF in podocytes in the pathogenesis of crescentic glomerulonephritis
阐明足细胞中 HIF 在新月体肾小球肾炎发病机制中的作用
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