Non-invasive assessment for teratogenic-toxicity of environmental disrupters by structural- and cel lurar-biological methods

通过结构生物学和细胞生物学方法对环境破坏物的致畸毒性进行非侵入性评估

• 批准号: 11556052
• 负责人: MANABE Noboru
• 金额: $ 8.64万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11556052/
• 关键词: environmental distruptors; reproductive toxicity; genetic toxicity; food chain; embryionic teratogenecity; reproductive physiology; genital gland; structural biology; 生体核磁気共鳴装置; 外因性内分泌攪乱物質; ステロイドホルモン; オーファン受容体; ステロイドホルモン受容体; 哺乳類生殖子; 哺乳類胎児

To develope non-invasive and real-time assesment technique for teratogenic activity of environmental disruptors (Eds), so called environmental hormones, we made a novel appl ication for nuclear magnetic resonance (NMR) microscopy to quantitatively observe of malmorphogenecity in mammal ian embryos. By using the new structural-biological equipment, that is magnetic resonance (NMR)-imaging appliance, developmental disorder inmammaIianembryos induced byoral maternal-administration of some Eds was non-invasiveIy detected. This MRI methods with an enhanced spatial resolution due to the use of a high static magnetic field and high magnetic field gradients. The NMR appl iance is a useful tool for non-invasive, real-time and continuous examination of tissue and organs at the histological level (minimum 50 micron cubic). We demonstrated that many Eds induced widespread malformation in developing mammal ian embryos, and that two-dimensional and/or three-dimensional NMR micro-images demonstrated defects in skeleton and soft tissue similarly to conventional histopathological assessments. Although further improvements are required for rapid dynamic imaging of living embryos in the peritoneal cavity of mother with Eds administration, our NMR-imaging technique will provide new approach as for multi-parametric assessment of embryo development under both physiological and pathological conditions.

为了发展环境干扰物（Environmental Disruptors，Eds）致畸活性的非侵入性实时检测技术，我们将核磁共振（NMR）显微镜应用于哺乳动物胚胎畸形发生的定量观察。本文应用新型结构生物学设备--核磁共振（NMR）成像仪，对母体口服某些Eds引起的哺乳动物胚胎发育障碍进行了无创性检测。由于使用高静磁场和高磁场梯度，这种MRI方法具有增强的空间分辨率。NMR应用程序是一种有用的工具，可在组织学水平（最小50微米立方）对组织和器官进行无创、实时和连续检查。我们证明，许多Eds在发育中的哺乳动物胚胎中引起广泛的畸形，并且二维和/或三维NMR显微图像显示骨骼和软组织的缺陷与常规组织病理学评估相似。虽然需要进一步改进的快速动态成像的活胚胎在腹腔内的母亲与EDS管理，我们的核磁共振成像技术将提供新的方法，多参数评估胚胎发育的生理和病理条件下。

项目成果

Noboru Manabe et al.: "Quantitative estimation of physiological properties by histochemical techniques. In: Miyamoto H, Manabe N (eds). Reproductive Biotechnology : Reproductive biotechnology update and its related physiology"Hokuto Publishing Co., Kyoto,

Noboru Manabe 等人：“通过组织化学技术定量估计生理特性。见：Miyamoto H、Manabe N（编）。生殖生物技术：生殖生物技术更新及其相关生理学”北斗出版公司，京都，

Miki SUGIMOTO, Noboru Manabe et al.: "Histological and biochemical profiles of granulosa cell apoptosis in follicular atresia of porcine ovaries. In : Miyamoto H, Manabe N (eds), Reproductive Biotechnology : Reproductive biotechnology update and its relat

Miki SUGIMOTO、Noboru Manabe 等人：“猪卵巢卵泡闭锁中颗粒细胞凋亡的组织学和生化特征。见：Miyamoto H、Manabe N（编辑），生殖生物技术：生殖生物技术更新及其相关

Maki Morita, Noboru Manabe et al.: "Functions of mammal ian oviducts and their relevance to morphology. In : Miyamoto H, Manabe N (eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology"Hokuto Publishing Co., Kyoto

Maki Morita、Noboru Manabe 等人：“哺乳动物输卵管的功能及其与形态的相关性。见：Miyamoto H、Manabe N（编辑），生殖生物技术：生殖生物技术更新及其相关生理学”北斗出版公司，京都

Miki Sugimoto, Noboru Manabe et al.: "Update on cryopreservation of mammalian ovaries"Obstetrics and Gynecology. 68. 207-212 (2001)

Miki Sugimoto、Noboru Manabe 等人：“哺乳动物卵巢冷冻保存的最新进展”妇产科。

Noboru Manabe et al.: "A novel cell death receptor and a decoy receptor on follicular granulose cells of porcine ovaries"Japanese Journal of Embryo Transfer. 23. 52-60 (2001)

Noboru Manabe 等人：“猪卵巢滤泡颗粒细胞上的新型细胞死亡受体和诱饵受体”日本胚胎移植杂志。