Development of estimation method for mammalian oocyte quality based on molecular mechanism of follicular selection

哺乳动物卵巢中有许多原始卵泡。不到1%发育并排卵，其余的通过闭锁排出。参与卵泡发育的内分泌机制已被表征，但参与卵泡选择调节的确切机制仍不清楚。最近的研究表明，颗粒细胞的凋亡在卵泡选择中起着至关重要的作用。值得注意的是，死亡配体和受体系统以及细胞内信号传导是调控颗粒细胞凋亡的关键机制。本研究揭示了死亡配体和受体系统及信号通路调控颗粒细胞凋亡的机制，以及凋亡抑制因子（细胞fliclike inhibitory protein: cFLIP和X-linked inhibitor of apoptosis protein: XIAP）在颗粒细胞凋亡中的作用。这些抑制剂可用于评估受损和/或健康的卵母细胞。

Many primordial follicles are present in the mammalian ovary. Less than 1% develop and ovulate, and the remainders are eliminated via atresia. The endocrinological mechanisms involved in follicular development have been characterized, but the precise mechanisms involved in the regulation of follicule selection remain unknown. Recent studies suggest that apoptosis in granulosa cells plays a crucial role in follicular selection. Notably, death ligand and receptor system and intracellular signaling are the key mechanisms regulating granulosa cell apoptosis. In this study, the mechanisms of granulosa cell apoptosis regulated by death ligand and receptor system and signaling, and the roles of apoptosis inhibitory factors (cellular FLICE-like inhibitory protein : cFLIP and X-linked inhibitor of apoptosis protein : XIAP) were revealed. The inhibitors are useful to assess damaged and/or healthy oocytes.