Development of estimation method for mammalian oocyte quality based on molecular mechanism of follicular selection

哺乳动物卵巢中存在许多原始卵泡。不到1%的人发育和排卵，并通过闭锁消除。参与卵泡发育的内分泌学机制已被表征，但参与卵泡选择调节的精确机制仍不清楚。最近的研究表明，颗粒细胞凋亡在卵泡选择中起着至关重要的作用。值得注意的是，死亡配体和受体系统和细胞内信号转导是调节颗粒细胞凋亡的关键机制。本研究主要探讨死亡配体和受体系统及信号转导对颗粒细胞凋亡的调控机制，以及凋亡抑制因子（细胞FLICE样抑制蛋白cFLIP和X连锁凋亡抑制蛋白XIAP）在颗粒细胞凋亡中的作用。所述抑制剂可用于评估受损和/或健康的卵母细胞。

Many primordial follicles are present in the mammalian ovary. Less than 1% develop and ovulate, and the remainders are eliminated via atresia. The endocrinological mechanisms involved in follicular development have been characterized, but the precise mechanisms involved in the regulation of follicule selection remain unknown. Recent studies suggest that apoptosis in granulosa cells plays a crucial role in follicular selection. Notably, death ligand and receptor system and intracellular signaling are the key mechanisms regulating granulosa cell apoptosis. In this study, the mechanisms of granulosa cell apoptosis regulated by death ligand and receptor system and signaling, and the roles of apoptosis inhibitory factors (cellular FLICE-like inhibitory protein : cFLIP and X-linked inhibitor of apoptosis protein : XIAP) were revealed. The inhibitors are useful to assess damaged and/or healthy oocytes.