Development of estimation method for mammalian oocyte quality based on molecular mechanism of follicular selection

基于卵泡选择分子机制的哺乳动物卵母细胞质量评价方法的建立

• 批准号: 18380164
• 负责人: MANABE Noboru
• 金额: $ 11.52万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380164/
• 关键词: 家畜繁殖; 卵胞選抜; 卵母細胞; アポトーシス; 細胞死阻害因子; 卵子品質; 卵胞閉鎖; 顆粒層細胞; 哺乳類; 卵巣; 発現制御; 卵胞; 細胞死受容体; 選択的死滅; カスパーゼ; アポトーシス阻害因子

Many primordial follicles are present in the mammalian ovary. Less than 1% develop and ovulate, and the remainders are eliminated via atresia. The endocrinological mechanisms involved in follicular development have been characterized, but the precise mechanisms involved in the regulation of follicule selection remain unknown. Recent studies suggest that apoptosis in granulosa cells plays a crucial role in follicular selection. Notably, death ligand and receptor system and intracellular signaling are the key mechanisms regulating granulosa cell apoptosis. In this study, the mechanisms of granulosa cell apoptosis regulated by death ligand and receptor system and signaling, and the roles of apoptosis inhibitory factors (cellular FLICE-like inhibitory protein : cFLIP and X-linked inhibitor of apoptosis protein : XIAP) were revealed. The inhibitors are useful to assess damaged and/or healthy oocytes.

哺乳动物卵巢中存在许多原始卵泡。不到1%的人发育和排卵，并通过闭锁消除。参与卵泡发育的内分泌学机制已被表征，但参与卵泡选择调节的精确机制仍不清楚。最近的研究表明，颗粒细胞凋亡在卵泡选择中起着至关重要的作用。值得注意的是，死亡配体和受体系统和细胞内信号转导是调节颗粒细胞凋亡的关键机制。本研究主要探讨死亡配体和受体系统及信号转导对颗粒细胞凋亡的调控机制，以及凋亡抑制因子（细胞FLICE样抑制蛋白cFLIP和X连锁凋亡抑制蛋白XIAP）在颗粒细胞凋亡中的作用。所述抑制剂可用于评估受损和/或健康的卵母细胞。

项目成果

犬の解剖・カラーリングアトラス

狗的解剖学和着色图集

• 发表时间: 2006
• 作者: Takashi Miyano;Noboru Manabe;眞鍋 昇;眞鍋 昇;眞鍋 昇
• 通讯作者: 眞鍋 昇

Incorporation of β-amyloid protein through the bovine ileal epithelium before and after weaning:: Model for orally transmitted amyloidoses

• DOI: 10.1111/j.1348-0421.2008.00054.x
• 发表时间: 2008-08-01
• 期刊: MICROBIOLOGY AND IMMUNOLOGY
• 影响因子: 2.6
• 作者: Ano, Yasuhisa;Nakayama, Hiroyuki;Onodera, Takashi
• 通讯作者: Onodera, Takashi

Urinary concentrations of estrone-3-glucuronide and estrous behavior in a captive female giant panda (Ailuropoda melanoleuca)

圈养雌性大熊猫（Ailuropoda melanoleuca）尿中雌酮-3-葡萄糖醛酸浓度和发情行为

• 发表时间: 2008
• 期刊: Journal of Reproduction and Development 54(4)
• 作者: Hama N;Kanemitsu H;Sakamoto K;Oyama Y;Acosta TJ;Ishikawa O;Pengyan W;Okuda K.
• 通讯作者: Okuda K.

Changes in expression and localization of GPRC5B and RAR in placenta and yolk sac during middle to late gestation in mice

小鼠妊娠中晚期胎盘和卵黄囊中GPRC5B和RAR表达及定位的变化

• 发表时间: 2007
• 期刊: Journal of Reproduction and Development 53
• 作者: Satoshi Imanishi;Miki Sugimoto;Maki Morita;Shinichi Kume;Noboru Manabe
• 通讯作者: Noboru Manabe

Cell death ligand and receptor dependent regulatory mechanisms of follicule selection in porcine ovaries

猪卵巢卵泡选择的细胞死亡配体和受体依赖性调节机制

• 发表时间: 2007
• 期刊: Morphology 2
• 作者: Noboru Manabe;Akihisa Maeda;Yasufumi Goto;Fuko Minehata;Naoko Inoue;Yuan Cheng;Huazi Jin;Kamika Wongpanit;Shoma Nakagawa;Jun-You Li;Takashi Miyano;Kazuhiro Sakamaki
• 通讯作者: Kazuhiro Sakamaki