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Preparation of calcium absorption-stimulating whey protein using Amino-carbonyl reaction

氨基-羰基反应制备促钙吸收乳清蛋白

基本信息

批准号：
07556146
负责人：
AOKI Takayoshi
金额：
$ 0.64万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556146/
关键词：
whey protein glucose 6-phosphate calcium absorption calcium phosphate Amino-carbonyl reaction

项目摘要

In order to examined the optimum conditions for the Millard reaction between whey protein and glucose-6-phosphate (G6P) were examined, whey protein and G6P were dissolved at various weight ratios at pH 7.0, freeze-dried, incubated at 50ﾟC and 65% relative humidity, and the browning, polymerization of proteins, the phosphorus content of reaction products were measured. The following conditions were appropriate : the ratio of whey protein to G6P,1 to 0.3 ; reaction time, less than 1 day, because brown color development and polymerization of protein exceed rapidly and the phosphorous content of whey protein-G6P conjugates prepared for 0.5 or 1 day of incubation was higher that of casein. The effect of the WPI (whey protein isolate) -G6P conjugate on calcium absorption was examined using rats. The proportions of soluble calcium in the ligated loop of the small intestine of the soy protein, casein, and WPI-G6P conjugate diet groups were 43.2(]SY.+-。[)8.7,72.7(]SY.+-。[)8.0, and 77.7(]SY.+-。[)9.7, respectively. The soluble calcium in the small intestine was significantly higher in the WPI-G6P conjugate diet group than in the soy protein diet group. This indicated that the WPI-G6P conjugate solubilizes calcium to prevent the precipitation of calcium phosphate in the small intestine. The calcium absorption, which was estimated by the disappearance of <@D145@>D1Ca in the ligated loop, of the soy protein, casein, and WPI-G6P conjugate diet groups was 50.7(]SY.+-。[)17.6,58.5(]SY.+-。[)8.3, and 72.6(]SY.+-。[)19.7, respectively. The calcium absorption was also higher in the WPI-G6P conjugate diet group than in the soy protein diet group. These results suggest that WPI-G6P conjugates are useful as food materials.

为探讨乳清蛋白与葡萄糖-6-磷酸(G6P)进行Millard反应的最佳条件，将乳清蛋白与G6P按不同质量比在pH7.0下溶解，冷冻干燥，在50ﾟC和65%相对湿度下孵育，测定了蛋白质的褐变、聚合和反应产物中的磷含量。适宜的工艺条件为：乳清蛋白与G6P的比例为1：0.3，反应时间不超过1d，因为蛋白质的棕色形成和聚合过快，且孵育0.5d或1d制备的乳清蛋白-G6P结合物的磷含量高于酪蛋白。以大鼠为实验对象，研究了乳清蛋白-G6P结合物对钙吸收的影响。大豆蛋白、酪蛋白和WPI-G6P结合饲料组的小肠结扎环中可溶性钙的比例为43.2(Sy.+-)。[)8.772.7(]SY+-.[)8.0和77.7()sy.+-。[)9.7。WPI-G6P结合饲料组小鼠小肠组织中的可溶性钙显著高于大豆蛋白饲料组。这表明WPI-G6P结合物溶解了钙，从而阻止了磷酸钙在小肠中的沉淀。大豆蛋白、酪蛋白和WPI-G6P结合饲料组的钙吸收率为50.7(SY+-)，通过结扎环中&lt；@D145@&gt；D1Ca的消失来估计。[)17.6，58.5(]正数+-。[)8.3和72.6()sy.+-。[)19.7。WPI-G6P结合饲料组的钙吸收也高于大豆蛋白饲料组。这些结果表明WPI-G6P偶联物是一种有用的食品原料。