Establishment of the system to study the function and therapy of deseases in human organs maintained in SCID mice

SCID小鼠人体器官功能及疾病治疗研究体系的建立

基本信息

  • 批准号:
    07558078
  • 负责人:
  • 金额:
    $ 8万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    1995
  • 资助国家:
    日本
  • 起止时间:
    1995 至 1997
  • 项目状态:
    已结题

项目摘要

We established an experimental system to investigate the development, morphology, physiology and disorder of human organ and tissue which are maintained in the severe combined immunodeficient mice (SCID) for long period.1. Long maintenance of normal human tissues and examination of the function.Normal human organs and tissues (bone marrow, skin, thyroid, colon, stomach, liver, lung, fetal tissues, etc.) were maintained for 2-3 years in the improved SCID mice. These human organs showed normal morphology and function. In particular, thyroid function remained active by the treatment with thyroid stimulating hormone (TSH). Labeled iodine was incorporated into human thyroid gland maintained in the SCID mice more actively by the injection of human TSH to the mice. Human embryonic tissues also well developed and grew rapidly in SCID mice.2. Experimental toxicology and therapy in human tissues.Human skin transplanted to the SCID mice were exposed to ultraviolet light B (UVB) for long period. UVB doses more than 5 x 10^5J/m^2 induced actinic keratosis in 75% of transplanted human skin. Human skin which were more sensitives for keratosis induction were also sensitive to UVB for cancer induction. Daily UVB doses more than 2,000 J/m^2 for 2 years (about 2 x 10^6 J/m^2 in total) induced 3 squqamous cell carcinomas in human skin. It was also possible to treat psoriasis and alopecia skin with human lymphocytes.
为了研究重度联合免疫缺陷小鼠(SCID)长期维持的人体器官和组织的发育、形态、生理和紊乱,我们建立了一套实验系统。人体正常组织的长期维护和功能的检查。改良SCID小鼠的正常人体器官和组织(骨髓、皮肤、甲状腺、结肠、胃、肝、肺、胎儿组织等)维持2-3年。这些人体器官表现出正常的形态和功能。特别是,甲状腺功能通过促甲状腺激素(TSH)治疗保持活跃。在SCID小鼠中,通过注射人TSH使标记碘更活跃地进入人甲状腺。SCID小鼠的人胚胎组织发育良好,生长迅速。人体组织的实验毒理学和治疗。将移植到SCID小鼠体内的人皮肤长期暴露在紫外线B (UVB)下。超过5 × 10^5J/m^2的UVB剂量在75%的移植皮肤中引起光化性角化病。对角化病诱导更敏感的人类皮肤对UVB诱导癌症也很敏感。每日UVB剂量超过2,000 J/m^2,持续2年(总计约2 × 10^6 J/m^2),诱发3例人体皮肤鳞状细胞癌。用人淋巴细胞治疗牛皮癣和脱发也是可能的。

项目成果

期刊论文数量(33)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kawguchi, T., et al.: "Consecutive maintenance of human solitary and hereditary colorectal polyps in SCID mice." Cancer Detection and Prevevtion. 21. 148-157 (1997)
Kawguchi, T. 等人:“在 SCID 小鼠中连续维持人类孤立性和遗传性结直肠息肉。”
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    0
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  • 通讯作者:
Li, L.Y., et al: "Dose rate effectiveness and potentially lethal damage repair in normal and double-strand break repair deficient murine cells by γ-rays and 5 fluorouracil." Cancer Letters. 123. 227-232 (1998)
Li, L.Y. 等人:“γ 射线和 5 氟尿嘧啶对正常和双链断裂修复缺陷小鼠细胞的剂量率有效性和潜在致命损伤修复”,123. 227-232 (1998)。
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    0
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Enomoto,T.: "Alteration of the p53 tumor suppressor gene and activation of c-K-ras-2 protooncogene in endometrial adenocarcinoma from Colorado." American J.Clinical Pathology,Anat.Pathology. 103. 224-230 (1995)
Enomoto,T.:“科罗拉多州子宫内膜腺癌中 p53 肿瘤抑制基因的改变和 c-K-ras-2 原癌基因的激活。”
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    0
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Nomura,T., et al.: "Induction of cancer,actinic keratosis and specific p53 mutations by ultraviolet light B in human skin maintained in SCID mice." Cancer Res.57. 2081-2084 (1997)
Nomura,T. 等人:“紫外线 B 在 SCID 小鼠的人类皮肤中诱导癌症、光化性角化病和特定 p53 突变。”
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  • 影响因子:
    0
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Enomoto,T.: "Loss of expression and loss of heterozygosity in the DCC gene in neoplasms of the human female reproductive tract." Brit.J.Cancer. 71. 462-467 (1995)
Enomoto,T.:“人类女性生殖道肿瘤中 DCC 基因的表达缺失和杂合性缺失。”
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  • 影响因子:
    0
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NOMURA Taisei其他文献

NOMURA Taisei的其他文献

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{{ truncateString('NOMURA Taisei', 18)}}的其他基金

Establishment of human benign prostatic hyperplasia model in super-SCID mice and its pathological analysis
人良性前列腺增生super-SCID小鼠模型的建立及病理分析
  • 批准号:
    26670196
  • 财政年份:
    2014
  • 资助金额:
    $ 8万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Direct effect of radiation and chemicals on human tissues and its mechanism
辐射和化学物质对人体组织的直接作用及其机制
  • 批准号:
    23241023
  • 财政年份:
    2011
  • 资助金额:
    $ 8万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
放射線、化学物質の継世代的(遺伝的)影響-マイクロサテライト突然変異誘発と疾病
辐射和化学物质的跨代(遗传)影响——微卫星突变和疾病
  • 批准号:
    20241014
  • 财政年份:
    2008
  • 资助金额:
    $ 8万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development and analysis of human organ/tissue damage by fission radionuclides
裂变放射性核素对人体器官/组织损伤的发展和分析
  • 批准号:
    16201013
  • 财政年份:
    2004
  • 资助金额:
    $ 8万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
EFFECT OF RADIATION EXPOSURE ON HUMAN ORGAN/TISSUE AND I'TS EXPERIMENTAL PROTECTION AND THERAPY
辐射暴露对人体器官/组织及其实验保护和治疗的影响
  • 批准号:
    12308029
  • 财政年份:
    2000
  • 资助金额:
    $ 8万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Genetic Effects of Low Dose Radiations in Man and Animals
低剂量辐射对人和动物的遗传效应
  • 批准号:
    08041174
  • 财政年份:
    1996
  • 资助金额:
    $ 8万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Dose and dose rate effectiveness to determine the risk of low dose radiation to human
确定低剂量辐射对人体风险的剂量和剂量率有效性
  • 批准号:
    07408031
  • 财政年份:
    1995
  • 资助金额:
    $ 8万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
"Man-Earth System : A scientific approach to realize human society in harmony with the Earth"-Transgenerational Effects of Man-made Environmental Toxic Agents Contaminating the Earth-
《人地系统:实现人类社会与地球和谐相处的科学途径》-人造环境有毒物质污染地球的跨代效应-
  • 批准号:
    05278114
  • 财政年份:
    1993
  • 资助金额:
    $ 8万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Radiation (Low Dose) -induced Apoptosis and It's Susceptibility Genes
辐射(低剂量)诱导的细胞凋亡及其易感基因
  • 批准号:
    04454606
  • 财政年份:
    1992
  • 资助金额:
    $ 8万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Induction of Congenital Anomalies in the Progeny After Germ Cell Exposure to Radiation and Chemicals.
生殖细胞暴露于辐射和化学物质后诱导后代先天性异常。
  • 批准号:
    63480506
  • 财政年份:
    1988
  • 资助金额:
    $ 8万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
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