Development and analysis of human organ/tissue damage by fission radionuclides

裂变放射性核素对人体器官/组织损伤的发展和分析

基本信息

  • 批准号:
    16201013
  • 负责人:
  • 金额:
    $ 31.53万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    2004
  • 资助国家:
    日本
  • 起止时间:
    2004 至 2006
  • 项目状态:
    已结题

项目摘要

1.Production of SCID mice with human organs and tissues (thyroid, bone marrow cells, lung, embryonic tissues)2.Effects of fission radionuclides on human tissues.(1) Dose rate of fission neutron and γ-rays from UTR-KINKI (1W, max 10^7n/cm^2-.sec) in the mouse body was measured; 0.2 Gy neutron and 0.2 Gy γ-rays per hour.(2) Weekly doses of 0.2 Gy of neutron were given 6 times to the transplated human thyroid tissues from Graves' disease patients and also 4 times to the human lung tissues grown from embryonic lung at 11 weeks. No p53, K-ras, c-kit, β-catenin, RET, bak, BRAF mutations were detected by these doses in the thyroid and lung tissues, while large doses (11-33 Gy) of ^<137>Cs γ-rays induced 8 mutations (6 p53 and 2 c-kit) in 20 irradiated human thyroid tissues. GeneChip analyses (8500 genes) revealed 4 fold increases or decreases of gene expression in 59.7 genes of thyroid tissues and 18.5 genes of lung tissues 1 week after exposure to neutrons (0.2 Gy × 4).3.Effects of ^<131>I : 0.5 MBq/mouse of ^<131>I was given weekly to SCID mice with human thyroid tissues. Six mutations were induced in 18 thyroid tissues exposed for more than 37 weeks, but only 2 mutations in 24 tissues for less than 36 weeks; 7 p53 and 1 β-catenin mutations. Single dose of 0.06-0.5 MBq/mouse of ^<131>I induced 4 fold increases or decreases in gene expression of 25-51.5 genes, suggesting very strong effects of radioiodine.4.In the liquidators exposed to radionuclides from the catastrophe of Chernobyl nuclear plant, abnormal overexpression of WT1 gene was observed and 2 became leukemia. Microsatellite mutation was also examined in the children of liquidators exposed to radionuclides.5.^<137>Cs γ-ray induced congenital malformation and leukemia were inhibited significantly by active hexose correlated compound.
1.用人体器官和组织(甲状腺、骨髓细胞、肺、胚胎组织)生产SCID小鼠2.裂变放射性核素对人体组织的影响。(1)测量了UTR-KINKI裂变中子和γ射线(1 W,最大10^7 n/cm ^2-.sec)在小鼠体内的剂量率;每小时0.2戈伊和0.2戈伊射线。(2)对移植的Graves病患者甲状腺组织和11周人胚肺组织分别给予每周剂量为0.2戈伊的中子照射6次和4次。大剂量(11-33戈伊)~(60)Cs γ射线照射20例甲状腺组织,未发现p53、K-ras、c-kit、β-catenin、RET、巴克、BRAF突变<137>。基因芯片分析(8500个基因)显示,在0.2戈伊× 4的中子照射后1周,甲状腺组织中有59.7个基因和肺组织中有18.5个基因的基因表达增加或减少了4倍<131><131>。在暴露超过37周的18个甲状腺组织中诱导了6个突变,但在暴露少于36周的24个组织中仅诱导了2个突变; 7个p53和1个β-catenin突变。0.06- 0.5MBq/只的单次剂量<131>的~(13)I可诱导25-51.5个基因表达增加或减少4倍,表明放射性碘具有很强的作用。4.在切尔诺贝利核电站事故放射性核素暴露的清理者中,观察到WT 1基因异常过表达,其中2例发生白血病。在暴露于放射性核素的清理者的子女中也检查了微卫星突变。<137>活性己糖相关化合物对Cs γ射线诱发的先天畸形和白血病有明显的抑制作用。

项目成果

期刊论文数量(124)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Critical role of RecA and RecF proteins in strand break rejoining and maintenance of fidelity of rejoining following γ-radiation-induced damage to pMTa4 DNA in E. coli
RecA 和 RecF 蛋白在大肠杆菌中 γ 辐射诱导的 pMTa4 DNA 损伤后链断裂重新连接和维持重新连接保真度中的关键作用
P53, K-ras, β-catenin, c-Kit and bak Mutations in Chineses and Japanese lung cancer cases.
中国和日本肺癌病例中的 P53、K-ras、β-catenin、c-Kit 和 bak 突变。
Male-mediated Developmental Toxicity (Chapter 12. Transmissible Genetic Risk Causing Tumours in Mice and Humans)
雄性介导的发育毒性(第 12 章。导致小鼠和人类肿瘤的传染性遗传风险)
Th1-biased humoral immune responses against Wilms tumor gene WT1 product in the patients with hematopoietic malignancies.
造血系统恶性肿瘤患者针对肾母细胞瘤基因WT1产物的Th1偏向体液免疫反应。
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Wu F;Oka Y;Tsuboi A;Elisseeva O. A;Nakajima H;Fujiki F;Masuda T;Murakami M;Yoshihara S;Ikegame K;Hosen N;Kawakami M;Nakagawa M;Kubota T;Soma T;Yamagami T;Tsukaguchi M;Ogawa H;Oji Y;Hamaoka T;Kawase I;Sugiyama H.
  • 通讯作者:
    Sugiyama H.
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NOMURA Taisei其他文献

NOMURA Taisei的其他文献

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{{ truncateString('NOMURA Taisei', 18)}}的其他基金

Establishment of human benign prostatic hyperplasia model in super-SCID mice and its pathological analysis
人良性前列腺增生super-SCID小鼠模型的建立及病理分析
  • 批准号:
    26670196
  • 财政年份:
    2014
  • 资助金额:
    $ 31.53万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Direct effect of radiation and chemicals on human tissues and its mechanism
辐射和化学物质对人体组织的直接作用及其机制
  • 批准号:
    23241023
  • 财政年份:
    2011
  • 资助金额:
    $ 31.53万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
放射線、化学物質の継世代的(遺伝的)影響-マイクロサテライト突然変異誘発と疾病
辐射和化学物质的跨代(遗传)影响——微卫星突变和疾病
  • 批准号:
    20241014
  • 财政年份:
    2008
  • 资助金额:
    $ 31.53万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
EFFECT OF RADIATION EXPOSURE ON HUMAN ORGAN/TISSUE AND I'TS EXPERIMENTAL PROTECTION AND THERAPY
辐射暴露对人体器官/组织及其实验保护和治疗的影响
  • 批准号:
    12308029
  • 财政年份:
    2000
  • 资助金额:
    $ 31.53万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Genetic Effects of Low Dose Radiations in Man and Animals
低剂量辐射对人和动物的遗传效应
  • 批准号:
    08041174
  • 财政年份:
    1996
  • 资助金额:
    $ 31.53万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Dose and dose rate effectiveness to determine the risk of low dose radiation to human
确定低剂量辐射对人体风险的剂量和剂量率有效性
  • 批准号:
    07408031
  • 财政年份:
    1995
  • 资助金额:
    $ 31.53万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Establishment of the system to study the function and therapy of deseases in human organs maintained in SCID mice
SCID小鼠人体器官功能及疾病治疗研究体系的建立
  • 批准号:
    07558078
  • 财政年份:
    1995
  • 资助金额:
    $ 31.53万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
"Man-Earth System : A scientific approach to realize human society in harmony with the Earth"-Transgenerational Effects of Man-made Environmental Toxic Agents Contaminating the Earth-
《人地系统:实现人类社会与地球和谐相处的科学途径》-人造环境有毒物质污染地球的跨代效应-
  • 批准号:
    05278114
  • 财政年份:
    1993
  • 资助金额:
    $ 31.53万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Radiation (Low Dose) -induced Apoptosis and It's Susceptibility Genes
辐射(低剂量)诱导的细胞凋亡及其易感基因
  • 批准号:
    04454606
  • 财政年份:
    1992
  • 资助金额:
    $ 31.53万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Induction of Congenital Anomalies in the Progeny After Germ Cell Exposure to Radiation and Chemicals.
生殖细胞暴露于辐射和化学物质后诱导后代先天性异常。
  • 批准号:
    63480506
  • 财政年份:
    1988
  • 资助金额:
    $ 31.53万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
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