Radiation (Low Dose) -induced Apoptosis and It's Susceptibility Genes

辐射（低剂量）诱导的细胞凋亡及其易感基因

基本信息

批准号：
04454606
负责人：
NOMURA Taisei
金额：
$ 4.1万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454606/
关键词：
Apoptosis Interphase Cell Death Mouse Thymus Low Dose Radiation Dominant Gene (Apo-1)Recessive Genes Programd Cell Death RI Strain 放射線酵素反応の介在

项目摘要

1) Dominant and recessive genes controling susceptibility to radiation induced apoptosis in the mouse thymus : We established sensitive method to detect apoptosis by staining dead or dying cells with erythrosines B in the frozen section of the organs (thymus, spleen, intestine, etc.). Single dominant gene showing Menderian inheritance was found by the genetic cross of radiation-induced apoptossis sensitive strain N4 and resistant C3H/HeJ.The examinations using linkage stocks and microsatellite markers revealed that this gene was linked to the chromosome 4. Another 2 recessive genes controling susceptibility were found by the use of recombinant inbred strain (BHX) of senstive strain C57BL/6J and resistant C3H/HeJ.2) Massive suicide deaths of thymic Iymphocytes : By the specific stainig method of the frozen sections, we found that thymic cells died massively but not singly at early stages (2-8 hours) after irradiattion. Dead cells were eliminated after then and it looks like single cell death 24 hours after irradation. Apoptosis of Iymphocytes in the cultured thymus was inhibited by low temparature and cyclophosphamide (inhibitor of protein synthesis) .3) Realation to Lymphocytic leukemia : Mouse strains showing high susceptibility to radiation-induced apoptosis are also susceptible to radiation-induced leukmia, but the analysis by RI strain showed that genes concerned to these disorders are different.

1）控制辐射诱导的小鼠胸腺凋亡的敏感性的显性和隐性基因：我们建立了敏感方法来检测凋亡，通过在器官的冷冻部分（胸腺，脾，肠，肠等）染色死亡或垂死的细胞，用红细胞生成B。通过辐射诱导的凋亡敏感菌株N4和抗性C3H/HEJ的遗传交叉发现了显示遗传性的单个显性基因。使用链接库存和微卫星标记的检查表明，该基因揭示了该基因与染色体4相关。另一个由2个凹陷基因控制的应变。 C57BL/6J和耐药性C3H/HEJ.2）胸腺膜细胞的大规模自杀死亡：通过冷冻切片的特定Stainig方法，我们发现胸腺细胞在辐照后在早期（2-8小时）并未大规模死亡，但在辐照后的早期（2-8小时）不会死亡。在此之后消除了死细胞，辐照后24小时看起来像单细胞死亡。低温和环磷酰胺（蛋白质合成的抑制剂）抑制培养胸腺中胞胞细胞的凋亡。3）对淋巴细胞性白血病的介绍：小鼠菌株：对辐射诱导的辐射诱导的高易感性也对辐射诱导的对辐射诱导的诱导的依次分析也很明显，但这些分析均与RI分析相关，RI是RII的分析，RI是RII的分析，RI是RII的分析。