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Radiation (Low Dose) -induced Apoptosis and It's Susceptibility Genes

辐射（低剂量）诱导的细胞凋亡及其易感基因

基本信息

批准号：
04454606
负责人：
NOMURA Taisei
金额：
$ 4.1万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454606/
关键词：
Apoptosis Interphase Cell Death Mouse Thymus Low Dose Radiation Dominant Gene (Apo-1)Recessive Genes Programd Cell Death RI Strain 放射線酵素反応の介在

项目摘要

1) Dominant and recessive genes controling susceptibility to radiation induced apoptosis in the mouse thymus : We established sensitive method to detect apoptosis by staining dead or dying cells with erythrosines B in the frozen section of the organs (thymus, spleen, intestine, etc.). Single dominant gene showing Menderian inheritance was found by the genetic cross of radiation-induced apoptossis sensitive strain N4 and resistant C3H/HeJ.The examinations using linkage stocks and microsatellite markers revealed that this gene was linked to the chromosome 4. Another 2 recessive genes controling susceptibility were found by the use of recombinant inbred strain (BHX) of senstive strain C57BL/6J and resistant C3H/HeJ.2) Massive suicide deaths of thymic Iymphocytes : By the specific stainig method of the frozen sections, we found that thymic cells died massively but not singly at early stages (2-8 hours) after irradiattion. Dead cells were eliminated after then and it looks like single cell death 24 hours after irradation. Apoptosis of Iymphocytes in the cultured thymus was inhibited by low temparature and cyclophosphamide (inhibitor of protein synthesis) .3) Realation to Lymphocytic leukemia : Mouse strains showing high susceptibility to radiation-induced apoptosis are also susceptible to radiation-induced leukmia, but the analysis by RI strain showed that genes concerned to these disorders are different.

1)控制辐射敏感性的显性和隐性基因诱导小鼠胸腺细胞凋亡：我们建立了灵敏的检测凋亡的方法，通过用红B染色器官（胸腺、脾、肠等）冰冻切片中死亡或垂死的细胞。通过对辐射诱变水稻感病品系N4与抗辐射水稻感病品系C3 H/HeJ的遗传杂交，发现了一对显性基因，该基因与第4染色体连锁。用敏感株C57 BL/6 J和抗性株C3 H/HeJ的重组近交系（BHX），又发现了2个控制敏感性的隐性基因。2）胸腺淋巴细胞的大量自杀性死亡：用冰冻切片特异染色法，发现胸腺细胞在照射后早期（2-8小时）大量死亡，但不是单个死亡。然后死亡细胞被消除，并且在照射后24小时看起来像单细胞死亡。低温和环磷酰胺（蛋白质合成抑制剂）可以抑制培养胸腺中淋巴细胞的凋亡。3）与淋巴细胞白血病的关系：对辐射诱导的细胞凋亡高度敏感的小鼠品系也容易发生辐射诱导的白血病，但RI品系的分析表明，与这些疾病有关的基因是不同的。