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Structure and Function of Xanthomonas campestris alpha-amylase

Structure and Function of Xanthomonas campestris alpha-amylase
野油菜黄单胞菌α-淀粉酶的结构和功能
批准号:
07650964
负责人:
ABE Jun-ichi
金额:
$1.34万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1995
资助国家:
日本
项目状态:
已结题
起止时间:
1995 至 1996

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中文摘要
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英文摘要
This study aimed to get further structure / function relationship of Xanthomonas campestris alpha-amylase and three results as below were obtained.1. Mutant enzymes of which the 6th loop in (alpha / beta) 8 barrel was shortened by 4,8, or 12 amino acid residues were created. The selectivity of enzymes toward glycogen increased fivefold and twentyfold in the case of 4 and 8 residues-deleted mutant enzyme, respectively. This suggests that the long 6th loop of X.campestris alpha-amylase caused the steric hindrance between enzyme and glycogen. It is possible to say that a new enzyme can be created by partial deletion of loop in alpha-amylase,2. X.campestris alpha-amylase was crystallized by the hanging drop method. Conditions were as follows ; enzyme concentration, 10 mg / ml ; pH 4.5 ; temperature, 20゚C ; precipitant in reservoir and drop, 15% and 10%, respectively, saturated ammonium sulfate in 10 mM sodium acetate (pH 4.5).3. Auto-oxidation of methionine 82 was suspected to be a reason for the inactivation of X.campestris alpha-amylase, thus, the residue was replaced by alanine. The mutant enzyme was found to be quite stable in the presence of oxidizer of high concentration.
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Perceptual processes and emotional responses of music: cross-cultural investigation and cognitive modeling
  • 批准号:
    15200019
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 资助金额:
    $32.03万
  • 财政年份:
    2003
  • 负责人:
    ABE Jun-ichi
  • 依托单位:
The relationship between perceptual organization and emotional effect of music : Cognitive modeling and cross-cultural investigation
  • 批准号:
    13610074
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $2.56万
  • 财政年份:
    2001
  • 负责人:
    ABE Jun-ichi
  • 依托单位:
Cognitive modeling of human music information processing
  • 批准号:
    11832001
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $2.3万
  • 财政年份:
    1999
  • 负责人:
    ABE Jun-ichi
  • 依托单位:
Development of computer network system for psychological experiments
  • 批准号:
    08551001
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 资助金额:
    $6.4万
  • 财政年份:
    1996
  • 负责人:
    ABE Jun-ichi
  • 依托单位:
海外基金